TINCR is not a non‐coding RNA but encodes a protein component of cornified epidermal keratinocytes

Long non‐coding RNAs have been implicated in the regulation of a plethora of biological processes, yet it has been challenging to verify that they are truly not coding for proteins. Terminal differentiation‐induced non‐coding RNA (TINCR) is a 3.7‐kilobase mRNA that is highly abundant in epidermal ke...

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Autores principales: Eckhart, Leopold, Lachner, Julia, Tschachler, Erwin, Rice, Robert H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Concise Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7187231/
https://www.ncbi.nlm.nih.gov/pubmed/32012357
http://dx.doi.org/10.1111/exd.14083
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author Eckhart, Leopold
Lachner, Julia
Tschachler, Erwin
Rice, Robert H.
author_facet Eckhart, Leopold
Lachner, Julia
Tschachler, Erwin
Rice, Robert H.
author_sort Eckhart, Leopold
collection PubMed
description Long non‐coding RNAs have been implicated in the regulation of a plethora of biological processes, yet it has been challenging to verify that they are truly not coding for proteins. Terminal differentiation‐induced non‐coding RNA (TINCR) is a 3.7‐kilobase mRNA that is highly abundant in epidermal keratinocytes prior to cornification. Here, we report the presence of an evolutionarily conserved open reading frame in TINCR and the identification of peptides derived from this open reading frame in the proteome of human stratum corneum. Our results demonstrate that TINCR is a protein‐coding RNA and suggest that the TINCR‐encoded protein is involved in keratinocyte cornification.
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spelling pubmed-71872312020-04-28 TINCR is not a non‐coding RNA but encodes a protein component of cornified epidermal keratinocytes Eckhart, Leopold Lachner, Julia Tschachler, Erwin Rice, Robert H. Exp Dermatol Concise Communication Long non‐coding RNAs have been implicated in the regulation of a plethora of biological processes, yet it has been challenging to verify that they are truly not coding for proteins. Terminal differentiation‐induced non‐coding RNA (TINCR) is a 3.7‐kilobase mRNA that is highly abundant in epidermal keratinocytes prior to cornification. Here, we report the presence of an evolutionarily conserved open reading frame in TINCR and the identification of peptides derived from this open reading frame in the proteome of human stratum corneum. Our results demonstrate that TINCR is a protein‐coding RNA and suggest that the TINCR‐encoded protein is involved in keratinocyte cornification. John Wiley and Sons Inc. 2020-02-18 2020-04 /pmc/articles/PMC7187231/ /pubmed/32012357 http://dx.doi.org/10.1111/exd.14083 Text en © 2020 The Authors. Experimental Dermatology published by John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Concise Communication
Eckhart, Leopold
Lachner, Julia
Tschachler, Erwin
Rice, Robert H.
TINCR is not a non‐coding RNA but encodes a protein component of cornified epidermal keratinocytes
title TINCR is not a non‐coding RNA but encodes a protein component of cornified epidermal keratinocytes
title_full TINCR is not a non‐coding RNA but encodes a protein component of cornified epidermal keratinocytes
title_fullStr TINCR is not a non‐coding RNA but encodes a protein component of cornified epidermal keratinocytes
title_full_unstemmed TINCR is not a non‐coding RNA but encodes a protein component of cornified epidermal keratinocytes
title_short TINCR is not a non‐coding RNA but encodes a protein component of cornified epidermal keratinocytes
title_sort tincr is not a non‐coding rna but encodes a protein component of cornified epidermal keratinocytes
topic Concise Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7187231/
https://www.ncbi.nlm.nih.gov/pubmed/32012357
http://dx.doi.org/10.1111/exd.14083
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