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Transcriptome profiling of developing testes and spermatogenesis in the Mongolian horse
BACKGROUND: Horse testis development and spermatogenesis are complex physiological processes. METHODS: To study these processes, three immature and three mature testes were collected from the Mongolian horse, and six libraries were established using high-throughput RNA sequencing technology (RNA-Seq...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7187496/ https://www.ncbi.nlm.nih.gov/pubmed/32345215 http://dx.doi.org/10.1186/s12863-020-00843-5 |
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author | Li, Bei He, Xiaolong Zhao, Yiping Bai, Dongyi Du, Ming Song, Lianjie Liu, Zhuang Yin, Zhenchen Manglai, Dugarjaviin |
author_facet | Li, Bei He, Xiaolong Zhao, Yiping Bai, Dongyi Du, Ming Song, Lianjie Liu, Zhuang Yin, Zhenchen Manglai, Dugarjaviin |
author_sort | Li, Bei |
collection | PubMed |
description | BACKGROUND: Horse testis development and spermatogenesis are complex physiological processes. METHODS: To study these processes, three immature and three mature testes were collected from the Mongolian horse, and six libraries were established using high-throughput RNA sequencing technology (RNA-Seq) to screen for genes related to testis development and spermatogenesis. RESULTS: A total of 16,237 upregulated genes and 8,641 downregulated genes were detected in the testis of the Mongolian horse. These genes play important roles in different developmental stages of spermatogenesis and testicular development. Five genes with alternative splicing events that may influence spermatogenesis and development of the testis were detected. GO (Gene ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analyses were performed for functional annotation of the differentially expressed genes. Pathways related to “spermatogenesis,” male gamete generation,” “spermatid development” and “oocyte meiosis” were significantly involved in different stages of testis development and spermatogenesis. CONCLUSION: Genes, pathways and alternative splicing events were identified with inferred functions in the process of spermatogenesis in the Mongolian horse. The identification of these differentially expressed genetic signatures improves our understanding of horse testis development and spermatogenesis. |
format | Online Article Text |
id | pubmed-7187496 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-71874962020-04-30 Transcriptome profiling of developing testes and spermatogenesis in the Mongolian horse Li, Bei He, Xiaolong Zhao, Yiping Bai, Dongyi Du, Ming Song, Lianjie Liu, Zhuang Yin, Zhenchen Manglai, Dugarjaviin BMC Genet Research Article BACKGROUND: Horse testis development and spermatogenesis are complex physiological processes. METHODS: To study these processes, three immature and three mature testes were collected from the Mongolian horse, and six libraries were established using high-throughput RNA sequencing technology (RNA-Seq) to screen for genes related to testis development and spermatogenesis. RESULTS: A total of 16,237 upregulated genes and 8,641 downregulated genes were detected in the testis of the Mongolian horse. These genes play important roles in different developmental stages of spermatogenesis and testicular development. Five genes with alternative splicing events that may influence spermatogenesis and development of the testis were detected. GO (Gene ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analyses were performed for functional annotation of the differentially expressed genes. Pathways related to “spermatogenesis,” male gamete generation,” “spermatid development” and “oocyte meiosis” were significantly involved in different stages of testis development and spermatogenesis. CONCLUSION: Genes, pathways and alternative splicing events were identified with inferred functions in the process of spermatogenesis in the Mongolian horse. The identification of these differentially expressed genetic signatures improves our understanding of horse testis development and spermatogenesis. BioMed Central 2020-04-28 /pmc/articles/PMC7187496/ /pubmed/32345215 http://dx.doi.org/10.1186/s12863-020-00843-5 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Li, Bei He, Xiaolong Zhao, Yiping Bai, Dongyi Du, Ming Song, Lianjie Liu, Zhuang Yin, Zhenchen Manglai, Dugarjaviin Transcriptome profiling of developing testes and spermatogenesis in the Mongolian horse |
title | Transcriptome profiling of developing testes and spermatogenesis in the Mongolian horse |
title_full | Transcriptome profiling of developing testes and spermatogenesis in the Mongolian horse |
title_fullStr | Transcriptome profiling of developing testes and spermatogenesis in the Mongolian horse |
title_full_unstemmed | Transcriptome profiling of developing testes and spermatogenesis in the Mongolian horse |
title_short | Transcriptome profiling of developing testes and spermatogenesis in the Mongolian horse |
title_sort | transcriptome profiling of developing testes and spermatogenesis in the mongolian horse |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7187496/ https://www.ncbi.nlm.nih.gov/pubmed/32345215 http://dx.doi.org/10.1186/s12863-020-00843-5 |
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