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ACAT2 Promotes Cell Proliferation and Associates with Malignant Progression in Colorectal Cancer

BACKGROUND AND AIMS: Colorectal cancer (CRC) is a major disease that threatens human health. It has been reported that the acyl-coenzyme A (CoA): cholesterol acyltransferase 2 (ACAT2) gene can promote the progression of hepatocellular carcinoma, but its function in CRC is still unclear. In this stud...

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Autores principales: Weng, Meilin, Zhang, Hao, Hou, Wenting, Sun, Zhirong, Zhong, Jing, Miao, Changhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7187938/
https://www.ncbi.nlm.nih.gov/pubmed/32425549
http://dx.doi.org/10.2147/OTT.S238973
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author Weng, Meilin
Zhang, Hao
Hou, Wenting
Sun, Zhirong
Zhong, Jing
Miao, Changhong
author_facet Weng, Meilin
Zhang, Hao
Hou, Wenting
Sun, Zhirong
Zhong, Jing
Miao, Changhong
author_sort Weng, Meilin
collection PubMed
description BACKGROUND AND AIMS: Colorectal cancer (CRC) is a major disease that threatens human health. It has been reported that the acyl-coenzyme A (CoA): cholesterol acyltransferase 2 (ACAT2) gene can promote the progression of hepatocellular carcinoma, but its function in CRC is still unclear. In this study, we aimed to elucidate the function of ACAT2 in CRC. METHODS: Western blot and qPCR were used to detect the relative level of ACAT2 in CRC tissue and adjacent non-cancerous tissues, and then the association between ACAT2 expression and the clinicopathological features and survival of CRC patients were assessed. The expression of ACAT2 in CT26 and DLD1 cells was down-regulated by siRNA, and the effects of ACAT2 knockdown on cell proliferation were examined. The inhibitory effects of ACAT2 knockdown were further confirmed by tumor growth assays in vivo. RESULTS: Our data showed that the expression of ACAT2 in CRC tissues was markedly higher than in adjacent non-cancerous tissues. The high expression of ACAT2 was significantly associated with tumor size, lymph node metastasis and clinical stage. The increased expression of ACAT2 was also significantly associated with worse 5-year overall survival of CRC patients. siRNA-mediated ACAT2 knockdown strongly inhibited CT26 and DLD1 cells proliferation and induced G0/G1 phase cell cycle arrest and apoptosis in these cells. Knockdown of ACAT2 expression suppressed the growth of CRC and inhibited the expression of Ki67 in vivo. CONCLUSION: Our study demonstrated that ACAT2 played a positive role in regulating the proliferation of CRC and may be useful as a potential biomarker and therapeutic target for this disease.
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spelling pubmed-71879382020-05-18 ACAT2 Promotes Cell Proliferation and Associates with Malignant Progression in Colorectal Cancer Weng, Meilin Zhang, Hao Hou, Wenting Sun, Zhirong Zhong, Jing Miao, Changhong Onco Targets Ther Original Research BACKGROUND AND AIMS: Colorectal cancer (CRC) is a major disease that threatens human health. It has been reported that the acyl-coenzyme A (CoA): cholesterol acyltransferase 2 (ACAT2) gene can promote the progression of hepatocellular carcinoma, but its function in CRC is still unclear. In this study, we aimed to elucidate the function of ACAT2 in CRC. METHODS: Western blot and qPCR were used to detect the relative level of ACAT2 in CRC tissue and adjacent non-cancerous tissues, and then the association between ACAT2 expression and the clinicopathological features and survival of CRC patients were assessed. The expression of ACAT2 in CT26 and DLD1 cells was down-regulated by siRNA, and the effects of ACAT2 knockdown on cell proliferation were examined. The inhibitory effects of ACAT2 knockdown were further confirmed by tumor growth assays in vivo. RESULTS: Our data showed that the expression of ACAT2 in CRC tissues was markedly higher than in adjacent non-cancerous tissues. The high expression of ACAT2 was significantly associated with tumor size, lymph node metastasis and clinical stage. The increased expression of ACAT2 was also significantly associated with worse 5-year overall survival of CRC patients. siRNA-mediated ACAT2 knockdown strongly inhibited CT26 and DLD1 cells proliferation and induced G0/G1 phase cell cycle arrest and apoptosis in these cells. Knockdown of ACAT2 expression suppressed the growth of CRC and inhibited the expression of Ki67 in vivo. CONCLUSION: Our study demonstrated that ACAT2 played a positive role in regulating the proliferation of CRC and may be useful as a potential biomarker and therapeutic target for this disease. Dove 2020-04-24 /pmc/articles/PMC7187938/ /pubmed/32425549 http://dx.doi.org/10.2147/OTT.S238973 Text en © 2020 Weng et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Weng, Meilin
Zhang, Hao
Hou, Wenting
Sun, Zhirong
Zhong, Jing
Miao, Changhong
ACAT2 Promotes Cell Proliferation and Associates with Malignant Progression in Colorectal Cancer
title ACAT2 Promotes Cell Proliferation and Associates with Malignant Progression in Colorectal Cancer
title_full ACAT2 Promotes Cell Proliferation and Associates with Malignant Progression in Colorectal Cancer
title_fullStr ACAT2 Promotes Cell Proliferation and Associates with Malignant Progression in Colorectal Cancer
title_full_unstemmed ACAT2 Promotes Cell Proliferation and Associates with Malignant Progression in Colorectal Cancer
title_short ACAT2 Promotes Cell Proliferation and Associates with Malignant Progression in Colorectal Cancer
title_sort acat2 promotes cell proliferation and associates with malignant progression in colorectal cancer
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7187938/
https://www.ncbi.nlm.nih.gov/pubmed/32425549
http://dx.doi.org/10.2147/OTT.S238973
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