Cargando…
The Pimpled Gold Nanosphere: A Superior Candidate for Plasmonic Photothermal Therapy
BACKGROUND: The development of highly efficient nanoparticles to convert light to heat for anti-cancer applications is quite a challenging field of research. METHODS: In this study, we synthesized unique pimpled gold nanospheres (PGNSs) for plasmonic photothermal therapy (PPTT). The light-to-heat co...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7188077/ https://www.ncbi.nlm.nih.gov/pubmed/32425523 http://dx.doi.org/10.2147/IJN.S248327 |
_version_ | 1783527264974012416 |
---|---|
author | Nasseri, Behzad Turk, Mustafa Kosemehmetoglu, Kemal Kaya, Murat Piskin, Erhan Rabiee, Navid Webster, Thomas J |
author_facet | Nasseri, Behzad Turk, Mustafa Kosemehmetoglu, Kemal Kaya, Murat Piskin, Erhan Rabiee, Navid Webster, Thomas J |
author_sort | Nasseri, Behzad |
collection | PubMed |
description | BACKGROUND: The development of highly efficient nanoparticles to convert light to heat for anti-cancer applications is quite a challenging field of research. METHODS: In this study, we synthesized unique pimpled gold nanospheres (PGNSs) for plasmonic photothermal therapy (PPTT). The light-to-heat conversion capability of PGNSs and PPTT damage at the cellular level were investigated using a tissue phantom model. The ability of PGNSs to induce robust cellular damage was studied during cytotoxicity tests on colorectal adenocarcinoma (DLD-1) and fibroblast cell lines. Further, a numerical model of plasmonic (COMSOL Multiphysics) properties was used with the PPTT experimental assays. RESULTS: A low cytotoxic effect of thiolated polyethylene glycol (SH-PEG(400)-SH-) was observed which improved the biocompatibility of PGNSs to maintain 89.4% cell viability during cytometry assays (in terms of fibroblast cells for 24 hrs at a concentration of 300 µg/mL). The heat generated from the nanoparticle-mediated phantom models resulted in ΔT=30°C, ΔT=23.1°C and ΔT=21°C for the PGNSs, AuNRs, and AuNPs, respectively (at a 300 µg/mL concentration and for 325 sec). For the in vitro assays of PPTT on cancer cells, the PGNS group induced a 68.78% lethality (apoptosis) on DLD-1 cells. Fluorescence microscopy results showed the destruction of cell membranes and nuclei for the PPTT group. Experiments further revealed a penetration depth of sufficient PPTT damage in a physical tumor model after hematoxylin and eosin (H&E) staining through pathological studies (at depths of 2, 3 and 4 cm). Severe structural damages were observed in the tissue model through an 808-nm laser exposed to the PGNSs. CONCLUSION: Collectively, such results show much promise for the use of the present PGNSs and photothermal therapy for numerous anti-cancer applications. |
format | Online Article Text |
id | pubmed-7188077 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-71880772020-05-18 The Pimpled Gold Nanosphere: A Superior Candidate for Plasmonic Photothermal Therapy Nasseri, Behzad Turk, Mustafa Kosemehmetoglu, Kemal Kaya, Murat Piskin, Erhan Rabiee, Navid Webster, Thomas J Int J Nanomedicine Original Research BACKGROUND: The development of highly efficient nanoparticles to convert light to heat for anti-cancer applications is quite a challenging field of research. METHODS: In this study, we synthesized unique pimpled gold nanospheres (PGNSs) for plasmonic photothermal therapy (PPTT). The light-to-heat conversion capability of PGNSs and PPTT damage at the cellular level were investigated using a tissue phantom model. The ability of PGNSs to induce robust cellular damage was studied during cytotoxicity tests on colorectal adenocarcinoma (DLD-1) and fibroblast cell lines. Further, a numerical model of plasmonic (COMSOL Multiphysics) properties was used with the PPTT experimental assays. RESULTS: A low cytotoxic effect of thiolated polyethylene glycol (SH-PEG(400)-SH-) was observed which improved the biocompatibility of PGNSs to maintain 89.4% cell viability during cytometry assays (in terms of fibroblast cells for 24 hrs at a concentration of 300 µg/mL). The heat generated from the nanoparticle-mediated phantom models resulted in ΔT=30°C, ΔT=23.1°C and ΔT=21°C for the PGNSs, AuNRs, and AuNPs, respectively (at a 300 µg/mL concentration and for 325 sec). For the in vitro assays of PPTT on cancer cells, the PGNS group induced a 68.78% lethality (apoptosis) on DLD-1 cells. Fluorescence microscopy results showed the destruction of cell membranes and nuclei for the PPTT group. Experiments further revealed a penetration depth of sufficient PPTT damage in a physical tumor model after hematoxylin and eosin (H&E) staining through pathological studies (at depths of 2, 3 and 4 cm). Severe structural damages were observed in the tissue model through an 808-nm laser exposed to the PGNSs. CONCLUSION: Collectively, such results show much promise for the use of the present PGNSs and photothermal therapy for numerous anti-cancer applications. Dove 2020-04-24 /pmc/articles/PMC7188077/ /pubmed/32425523 http://dx.doi.org/10.2147/IJN.S248327 Text en © 2020 Nasseri et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Nasseri, Behzad Turk, Mustafa Kosemehmetoglu, Kemal Kaya, Murat Piskin, Erhan Rabiee, Navid Webster, Thomas J The Pimpled Gold Nanosphere: A Superior Candidate for Plasmonic Photothermal Therapy |
title | The Pimpled Gold Nanosphere: A Superior Candidate for Plasmonic Photothermal Therapy |
title_full | The Pimpled Gold Nanosphere: A Superior Candidate for Plasmonic Photothermal Therapy |
title_fullStr | The Pimpled Gold Nanosphere: A Superior Candidate for Plasmonic Photothermal Therapy |
title_full_unstemmed | The Pimpled Gold Nanosphere: A Superior Candidate for Plasmonic Photothermal Therapy |
title_short | The Pimpled Gold Nanosphere: A Superior Candidate for Plasmonic Photothermal Therapy |
title_sort | pimpled gold nanosphere: a superior candidate for plasmonic photothermal therapy |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7188077/ https://www.ncbi.nlm.nih.gov/pubmed/32425523 http://dx.doi.org/10.2147/IJN.S248327 |
work_keys_str_mv | AT nasseribehzad thepimpledgoldnanosphereasuperiorcandidateforplasmonicphotothermaltherapy AT turkmustafa thepimpledgoldnanosphereasuperiorcandidateforplasmonicphotothermaltherapy AT kosemehmetoglukemal thepimpledgoldnanosphereasuperiorcandidateforplasmonicphotothermaltherapy AT kayamurat thepimpledgoldnanosphereasuperiorcandidateforplasmonicphotothermaltherapy AT piskinerhan thepimpledgoldnanosphereasuperiorcandidateforplasmonicphotothermaltherapy AT rabieenavid thepimpledgoldnanosphereasuperiorcandidateforplasmonicphotothermaltherapy AT websterthomasj thepimpledgoldnanosphereasuperiorcandidateforplasmonicphotothermaltherapy AT nasseribehzad pimpledgoldnanosphereasuperiorcandidateforplasmonicphotothermaltherapy AT turkmustafa pimpledgoldnanosphereasuperiorcandidateforplasmonicphotothermaltherapy AT kosemehmetoglukemal pimpledgoldnanosphereasuperiorcandidateforplasmonicphotothermaltherapy AT kayamurat pimpledgoldnanosphereasuperiorcandidateforplasmonicphotothermaltherapy AT piskinerhan pimpledgoldnanosphereasuperiorcandidateforplasmonicphotothermaltherapy AT rabieenavid pimpledgoldnanosphereasuperiorcandidateforplasmonicphotothermaltherapy AT websterthomasj pimpledgoldnanosphereasuperiorcandidateforplasmonicphotothermaltherapy |