Expression of lamin C2 in mammalian oocytes

Lamin C2 (LMN C2) is a short product of the lamin a gene. It is a germ cell-specific lamin and has been extensively studied in male germ cells. In this study, we focussed on the expression and localization of LMN C2 in fully-grown germinal vesicle (GV) oocytes. We detected LMN C2 in the fully-grown...

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Autores principales: Koncicka, Marketa, Cervenka, Jakub, Jahn, Daniel, Sucha, Rita, Vodicka, Petr, Gad, Ahmed, Alsheimer, Manfred, Susor, Andrej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7188254/
https://www.ncbi.nlm.nih.gov/pubmed/32343699
http://dx.doi.org/10.1371/journal.pone.0229781
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author Koncicka, Marketa
Cervenka, Jakub
Jahn, Daniel
Sucha, Rita
Vodicka, Petr
Gad, Ahmed
Alsheimer, Manfred
Susor, Andrej
author_facet Koncicka, Marketa
Cervenka, Jakub
Jahn, Daniel
Sucha, Rita
Vodicka, Petr
Gad, Ahmed
Alsheimer, Manfred
Susor, Andrej
author_sort Koncicka, Marketa
collection PubMed
description Lamin C2 (LMN C2) is a short product of the lamin a gene. It is a germ cell-specific lamin and has been extensively studied in male germ cells. In this study, we focussed on the expression and localization of LMN C2 in fully-grown germinal vesicle (GV) oocytes. We detected LMN C2 in the fully-grown germinal vesicle oocytes of various mammalian species with confirmation done by immunoblotting the wild type and Lmnc2 gene deleted testes. Expression of LMN C2 tagged with GFP showed localization of LMN C2 to the nuclear membrane of the oocyte. Moreover, the LMN C2 protein notably disappeared after nuclear envelope breakdown (NEBD) and the expression of LMN C2 was significantly reduced in the oocytes from aged females and ceased altogether during meiotic maturation. These results provide new insights regarding LMN C2 expression in the oocytes of various mammalian species.
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spelling pubmed-71882542020-05-06 Expression of lamin C2 in mammalian oocytes Koncicka, Marketa Cervenka, Jakub Jahn, Daniel Sucha, Rita Vodicka, Petr Gad, Ahmed Alsheimer, Manfred Susor, Andrej PLoS One Research Article Lamin C2 (LMN C2) is a short product of the lamin a gene. It is a germ cell-specific lamin and has been extensively studied in male germ cells. In this study, we focussed on the expression and localization of LMN C2 in fully-grown germinal vesicle (GV) oocytes. We detected LMN C2 in the fully-grown germinal vesicle oocytes of various mammalian species with confirmation done by immunoblotting the wild type and Lmnc2 gene deleted testes. Expression of LMN C2 tagged with GFP showed localization of LMN C2 to the nuclear membrane of the oocyte. Moreover, the LMN C2 protein notably disappeared after nuclear envelope breakdown (NEBD) and the expression of LMN C2 was significantly reduced in the oocytes from aged females and ceased altogether during meiotic maturation. These results provide new insights regarding LMN C2 expression in the oocytes of various mammalian species. Public Library of Science 2020-04-28 /pmc/articles/PMC7188254/ /pubmed/32343699 http://dx.doi.org/10.1371/journal.pone.0229781 Text en © 2020 Koncicka et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Koncicka, Marketa
Cervenka, Jakub
Jahn, Daniel
Sucha, Rita
Vodicka, Petr
Gad, Ahmed
Alsheimer, Manfred
Susor, Andrej
Expression of lamin C2 in mammalian oocytes
title Expression of lamin C2 in mammalian oocytes
title_full Expression of lamin C2 in mammalian oocytes
title_fullStr Expression of lamin C2 in mammalian oocytes
title_full_unstemmed Expression of lamin C2 in mammalian oocytes
title_short Expression of lamin C2 in mammalian oocytes
title_sort expression of lamin c2 in mammalian oocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7188254/
https://www.ncbi.nlm.nih.gov/pubmed/32343699
http://dx.doi.org/10.1371/journal.pone.0229781
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