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Extracellular electron transfer-dependent anaerobic oxidation of ammonium by anammox bacteria

Anaerobic ammonium oxidation (anammox) bacteria contribute significantly to the global nitrogen cycle and play a major role in sustainable wastewater treatment. Anammox bacteria convert ammonium (NH(4)(+)) to dinitrogen gas (N(2)) using intracellular electron acceptors such as nitrite (NO(2)(−)) or...

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Autores principales: Shaw, Dario R., Ali, Muhammad, Katuri, Krishna P., Gralnick, Jeffrey A., Reimann, Joachim, Mesman, Rob, van Niftrik, Laura, Jetten, Mike S. M., Saikaly, Pascal E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7188810/
https://www.ncbi.nlm.nih.gov/pubmed/32345973
http://dx.doi.org/10.1038/s41467-020-16016-y
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author Shaw, Dario R.
Ali, Muhammad
Katuri, Krishna P.
Gralnick, Jeffrey A.
Reimann, Joachim
Mesman, Rob
van Niftrik, Laura
Jetten, Mike S. M.
Saikaly, Pascal E.
author_facet Shaw, Dario R.
Ali, Muhammad
Katuri, Krishna P.
Gralnick, Jeffrey A.
Reimann, Joachim
Mesman, Rob
van Niftrik, Laura
Jetten, Mike S. M.
Saikaly, Pascal E.
author_sort Shaw, Dario R.
collection PubMed
description Anaerobic ammonium oxidation (anammox) bacteria contribute significantly to the global nitrogen cycle and play a major role in sustainable wastewater treatment. Anammox bacteria convert ammonium (NH(4)(+)) to dinitrogen gas (N(2)) using intracellular electron acceptors such as nitrite (NO(2)(−)) or nitric oxide (NO). However, it is still unknown whether anammox bacteria have extracellular electron transfer (EET) capability with transfer of electrons to insoluble extracellular electron acceptors. Here we show that freshwater and marine anammox bacteria couple the oxidation of NH(4)(+) with transfer of electrons to insoluble extracellular electron acceptors such as graphene oxide or electrodes in microbial electrolysis cells. (15)N-labeling experiments revealed that NH(4)(+) was oxidized to N(2) via hydroxylamine (NH(2)OH) as intermediate, and comparative transcriptomics analysis revealed an alternative pathway for NH(4)(+) oxidation with electrode as electron acceptor. Complete NH(4)(+) oxidation to N(2) without accumulation of NO(2)(−) and NO(3)(−) was achieved in EET-dependent anammox. These findings are promising in the context of implementing EET-dependent anammox process for energy-efficient treatment of nitrogen.
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spelling pubmed-71888102020-05-01 Extracellular electron transfer-dependent anaerobic oxidation of ammonium by anammox bacteria Shaw, Dario R. Ali, Muhammad Katuri, Krishna P. Gralnick, Jeffrey A. Reimann, Joachim Mesman, Rob van Niftrik, Laura Jetten, Mike S. M. Saikaly, Pascal E. Nat Commun Article Anaerobic ammonium oxidation (anammox) bacteria contribute significantly to the global nitrogen cycle and play a major role in sustainable wastewater treatment. Anammox bacteria convert ammonium (NH(4)(+)) to dinitrogen gas (N(2)) using intracellular electron acceptors such as nitrite (NO(2)(−)) or nitric oxide (NO). However, it is still unknown whether anammox bacteria have extracellular electron transfer (EET) capability with transfer of electrons to insoluble extracellular electron acceptors. Here we show that freshwater and marine anammox bacteria couple the oxidation of NH(4)(+) with transfer of electrons to insoluble extracellular electron acceptors such as graphene oxide or electrodes in microbial electrolysis cells. (15)N-labeling experiments revealed that NH(4)(+) was oxidized to N(2) via hydroxylamine (NH(2)OH) as intermediate, and comparative transcriptomics analysis revealed an alternative pathway for NH(4)(+) oxidation with electrode as electron acceptor. Complete NH(4)(+) oxidation to N(2) without accumulation of NO(2)(−) and NO(3)(−) was achieved in EET-dependent anammox. These findings are promising in the context of implementing EET-dependent anammox process for energy-efficient treatment of nitrogen. Nature Publishing Group UK 2020-04-28 /pmc/articles/PMC7188810/ /pubmed/32345973 http://dx.doi.org/10.1038/s41467-020-16016-y Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Shaw, Dario R.
Ali, Muhammad
Katuri, Krishna P.
Gralnick, Jeffrey A.
Reimann, Joachim
Mesman, Rob
van Niftrik, Laura
Jetten, Mike S. M.
Saikaly, Pascal E.
Extracellular electron transfer-dependent anaerobic oxidation of ammonium by anammox bacteria
title Extracellular electron transfer-dependent anaerobic oxidation of ammonium by anammox bacteria
title_full Extracellular electron transfer-dependent anaerobic oxidation of ammonium by anammox bacteria
title_fullStr Extracellular electron transfer-dependent anaerobic oxidation of ammonium by anammox bacteria
title_full_unstemmed Extracellular electron transfer-dependent anaerobic oxidation of ammonium by anammox bacteria
title_short Extracellular electron transfer-dependent anaerobic oxidation of ammonium by anammox bacteria
title_sort extracellular electron transfer-dependent anaerobic oxidation of ammonium by anammox bacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7188810/
https://www.ncbi.nlm.nih.gov/pubmed/32345973
http://dx.doi.org/10.1038/s41467-020-16016-y
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