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Extracellular electron transfer-dependent anaerobic oxidation of ammonium by anammox bacteria
Anaerobic ammonium oxidation (anammox) bacteria contribute significantly to the global nitrogen cycle and play a major role in sustainable wastewater treatment. Anammox bacteria convert ammonium (NH(4)(+)) to dinitrogen gas (N(2)) using intracellular electron acceptors such as nitrite (NO(2)(−)) or...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7188810/ https://www.ncbi.nlm.nih.gov/pubmed/32345973 http://dx.doi.org/10.1038/s41467-020-16016-y |
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author | Shaw, Dario R. Ali, Muhammad Katuri, Krishna P. Gralnick, Jeffrey A. Reimann, Joachim Mesman, Rob van Niftrik, Laura Jetten, Mike S. M. Saikaly, Pascal E. |
author_facet | Shaw, Dario R. Ali, Muhammad Katuri, Krishna P. Gralnick, Jeffrey A. Reimann, Joachim Mesman, Rob van Niftrik, Laura Jetten, Mike S. M. Saikaly, Pascal E. |
author_sort | Shaw, Dario R. |
collection | PubMed |
description | Anaerobic ammonium oxidation (anammox) bacteria contribute significantly to the global nitrogen cycle and play a major role in sustainable wastewater treatment. Anammox bacteria convert ammonium (NH(4)(+)) to dinitrogen gas (N(2)) using intracellular electron acceptors such as nitrite (NO(2)(−)) or nitric oxide (NO). However, it is still unknown whether anammox bacteria have extracellular electron transfer (EET) capability with transfer of electrons to insoluble extracellular electron acceptors. Here we show that freshwater and marine anammox bacteria couple the oxidation of NH(4)(+) with transfer of electrons to insoluble extracellular electron acceptors such as graphene oxide or electrodes in microbial electrolysis cells. (15)N-labeling experiments revealed that NH(4)(+) was oxidized to N(2) via hydroxylamine (NH(2)OH) as intermediate, and comparative transcriptomics analysis revealed an alternative pathway for NH(4)(+) oxidation with electrode as electron acceptor. Complete NH(4)(+) oxidation to N(2) without accumulation of NO(2)(−) and NO(3)(−) was achieved in EET-dependent anammox. These findings are promising in the context of implementing EET-dependent anammox process for energy-efficient treatment of nitrogen. |
format | Online Article Text |
id | pubmed-7188810 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-71888102020-05-01 Extracellular electron transfer-dependent anaerobic oxidation of ammonium by anammox bacteria Shaw, Dario R. Ali, Muhammad Katuri, Krishna P. Gralnick, Jeffrey A. Reimann, Joachim Mesman, Rob van Niftrik, Laura Jetten, Mike S. M. Saikaly, Pascal E. Nat Commun Article Anaerobic ammonium oxidation (anammox) bacteria contribute significantly to the global nitrogen cycle and play a major role in sustainable wastewater treatment. Anammox bacteria convert ammonium (NH(4)(+)) to dinitrogen gas (N(2)) using intracellular electron acceptors such as nitrite (NO(2)(−)) or nitric oxide (NO). However, it is still unknown whether anammox bacteria have extracellular electron transfer (EET) capability with transfer of electrons to insoluble extracellular electron acceptors. Here we show that freshwater and marine anammox bacteria couple the oxidation of NH(4)(+) with transfer of electrons to insoluble extracellular electron acceptors such as graphene oxide or electrodes in microbial electrolysis cells. (15)N-labeling experiments revealed that NH(4)(+) was oxidized to N(2) via hydroxylamine (NH(2)OH) as intermediate, and comparative transcriptomics analysis revealed an alternative pathway for NH(4)(+) oxidation with electrode as electron acceptor. Complete NH(4)(+) oxidation to N(2) without accumulation of NO(2)(−) and NO(3)(−) was achieved in EET-dependent anammox. These findings are promising in the context of implementing EET-dependent anammox process for energy-efficient treatment of nitrogen. Nature Publishing Group UK 2020-04-28 /pmc/articles/PMC7188810/ /pubmed/32345973 http://dx.doi.org/10.1038/s41467-020-16016-y Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Shaw, Dario R. Ali, Muhammad Katuri, Krishna P. Gralnick, Jeffrey A. Reimann, Joachim Mesman, Rob van Niftrik, Laura Jetten, Mike S. M. Saikaly, Pascal E. Extracellular electron transfer-dependent anaerobic oxidation of ammonium by anammox bacteria |
title | Extracellular electron transfer-dependent anaerobic oxidation of ammonium by anammox bacteria |
title_full | Extracellular electron transfer-dependent anaerobic oxidation of ammonium by anammox bacteria |
title_fullStr | Extracellular electron transfer-dependent anaerobic oxidation of ammonium by anammox bacteria |
title_full_unstemmed | Extracellular electron transfer-dependent anaerobic oxidation of ammonium by anammox bacteria |
title_short | Extracellular electron transfer-dependent anaerobic oxidation of ammonium by anammox bacteria |
title_sort | extracellular electron transfer-dependent anaerobic oxidation of ammonium by anammox bacteria |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7188810/ https://www.ncbi.nlm.nih.gov/pubmed/32345973 http://dx.doi.org/10.1038/s41467-020-16016-y |
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