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Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks ( Capra hircus )

The Saanen goat breed has been widely explored in breeding programmes; however, there are few reports about the breed’s genetic and molecular composition. Thus, this study aimed to characterize the proteomic profile of spermatozoa from Saanen breeding goats. Five breeding animals with proven fertili...

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Autores principales: Pinto, Tatiana Maria Farias, Moreira, Raulzito Fernandes, Matos, Maria Nagila Carneiro, Soares, Vitória Virginia Magalhães, Aguiar, Mônica Valeria de Almeida, de Aragão, Paulo de Tarso Teles Dourado, Alves, João Garcia, Moreno, Frederico Bruno Mendes Batista, Monteiro-Moreira, Ana Cristina de Oliveira, Costa, Cíntia Renata Rocha, de Lima, José Luiz, Eloy, Angela Maria Xavier, da Cunha, Rodrigo Maranguape Silva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Colégio Brasileiro de Reprodução Animal 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189512/
https://www.ncbi.nlm.nih.gov/pubmed/32368270
http://dx.doi.org/10.21451/1984-3143-AR2019-0001
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author Pinto, Tatiana Maria Farias
Moreira, Raulzito Fernandes
Matos, Maria Nagila Carneiro
Soares, Vitória Virginia Magalhães
Aguiar, Mônica Valeria de Almeida
de Aragão, Paulo de Tarso Teles Dourado
Alves, João Garcia
Moreno, Frederico Bruno Mendes Batista
Monteiro-Moreira, Ana Cristina de Oliveira
Costa, Cíntia Renata Rocha
de Lima, José Luiz
Eloy, Angela Maria Xavier
da Cunha, Rodrigo Maranguape Silva
author_facet Pinto, Tatiana Maria Farias
Moreira, Raulzito Fernandes
Matos, Maria Nagila Carneiro
Soares, Vitória Virginia Magalhães
Aguiar, Mônica Valeria de Almeida
de Aragão, Paulo de Tarso Teles Dourado
Alves, João Garcia
Moreno, Frederico Bruno Mendes Batista
Monteiro-Moreira, Ana Cristina de Oliveira
Costa, Cíntia Renata Rocha
de Lima, José Luiz
Eloy, Angela Maria Xavier
da Cunha, Rodrigo Maranguape Silva
author_sort Pinto, Tatiana Maria Farias
collection PubMed
description The Saanen goat breed has been widely explored in breeding programmes; however, there are few reports about the breed’s genetic and molecular composition. Thus, this study aimed to characterize the proteomic profile of spermatozoa from Saanen breeding goats. Five breeding animals with proven fertility were selected, the spermatozoa were collected, and the protein was extracted. Subsequently, the proteins were separated and analysed by two-dimensional electrophoresis and mass spectrometry; the proteins were then identified with the SwissProt database. A total of 31 proteins involved in reproduction were identified, including binding proteins on spermatozoa for fusion with the egg, acrosomal membrane proteins, metabolic enzymes, heat shock proteins, cytoskeletal proteins and spermatozoa motility proteins. The characterization of such proteins clarifies the molecular mechanisms of spermatogenesis and the modifications that ensure the success of fertilization.
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spelling pubmed-71895122020-05-04 Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks ( Capra hircus ) Pinto, Tatiana Maria Farias Moreira, Raulzito Fernandes Matos, Maria Nagila Carneiro Soares, Vitória Virginia Magalhães Aguiar, Mônica Valeria de Almeida de Aragão, Paulo de Tarso Teles Dourado Alves, João Garcia Moreno, Frederico Bruno Mendes Batista Monteiro-Moreira, Ana Cristina de Oliveira Costa, Cíntia Renata Rocha de Lima, José Luiz Eloy, Angela Maria Xavier da Cunha, Rodrigo Maranguape Silva Anim Reprod Original Article The Saanen goat breed has been widely explored in breeding programmes; however, there are few reports about the breed’s genetic and molecular composition. Thus, this study aimed to characterize the proteomic profile of spermatozoa from Saanen breeding goats. Five breeding animals with proven fertility were selected, the spermatozoa were collected, and the protein was extracted. Subsequently, the proteins were separated and analysed by two-dimensional electrophoresis and mass spectrometry; the proteins were then identified with the SwissProt database. A total of 31 proteins involved in reproduction were identified, including binding proteins on spermatozoa for fusion with the egg, acrosomal membrane proteins, metabolic enzymes, heat shock proteins, cytoskeletal proteins and spermatozoa motility proteins. The characterization of such proteins clarifies the molecular mechanisms of spermatogenesis and the modifications that ensure the success of fertilization. Colégio Brasileiro de Reprodução Animal 2019-11-18 /pmc/articles/PMC7189512/ /pubmed/32368270 http://dx.doi.org/10.21451/1984-3143-AR2019-0001 Text en Copyright © The Author(s). http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Pinto, Tatiana Maria Farias
Moreira, Raulzito Fernandes
Matos, Maria Nagila Carneiro
Soares, Vitória Virginia Magalhães
Aguiar, Mônica Valeria de Almeida
de Aragão, Paulo de Tarso Teles Dourado
Alves, João Garcia
Moreno, Frederico Bruno Mendes Batista
Monteiro-Moreira, Ana Cristina de Oliveira
Costa, Cíntia Renata Rocha
de Lima, José Luiz
Eloy, Angela Maria Xavier
da Cunha, Rodrigo Maranguape Silva
Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks ( Capra hircus )
title Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks ( Capra hircus )
title_full Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks ( Capra hircus )
title_fullStr Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks ( Capra hircus )
title_full_unstemmed Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks ( Capra hircus )
title_short Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks ( Capra hircus )
title_sort evaluation of the proteomic profiles of ejaculated spermatozoa from saanen bucks ( capra hircus )
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189512/
https://www.ncbi.nlm.nih.gov/pubmed/32368270
http://dx.doi.org/10.21451/1984-3143-AR2019-0001
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