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Dissection of Larval and Pupal Wings of Bicyclus anynana Butterflies

The colorful wings of butterflies are emerging as model systems for evolutionary and developmental studies. Some of these studies focus on localizing gene transcripts and proteins in wings at the larval and pupal stages using techniques such as immunostaining and in situ hybridization. Other studies...

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Autores principales: Banerjee, Tirtha Das, Monteiro, Antónia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189656/
https://www.ncbi.nlm.nih.gov/pubmed/31936719
http://dx.doi.org/10.3390/mps3010005
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author Banerjee, Tirtha Das
Monteiro, Antónia
author_facet Banerjee, Tirtha Das
Monteiro, Antónia
author_sort Banerjee, Tirtha Das
collection PubMed
description The colorful wings of butterflies are emerging as model systems for evolutionary and developmental studies. Some of these studies focus on localizing gene transcripts and proteins in wings at the larval and pupal stages using techniques such as immunostaining and in situ hybridization. Other studies quantify mRNA expression levels or identify regions of open chromatin that are bound by proteins at different stages of wing development. All these techniques require dissection of the wings from the animal but a detailed video protocol describing this procedure has not been available until now. Here, we present a written and accompanying video protocol where we describe the tools and the method we use to remove the larval and pupal wings of the African Squinting Bush Brown butterfly Bicyclus anynana. This protocol should be easy to adapt to other species.
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spelling pubmed-71896562020-05-01 Dissection of Larval and Pupal Wings of Bicyclus anynana Butterflies Banerjee, Tirtha Das Monteiro, Antónia Methods Protoc Protocol The colorful wings of butterflies are emerging as model systems for evolutionary and developmental studies. Some of these studies focus on localizing gene transcripts and proteins in wings at the larval and pupal stages using techniques such as immunostaining and in situ hybridization. Other studies quantify mRNA expression levels or identify regions of open chromatin that are bound by proteins at different stages of wing development. All these techniques require dissection of the wings from the animal but a detailed video protocol describing this procedure has not been available until now. Here, we present a written and accompanying video protocol where we describe the tools and the method we use to remove the larval and pupal wings of the African Squinting Bush Brown butterfly Bicyclus anynana. This protocol should be easy to adapt to other species. MDPI 2020-01-10 /pmc/articles/PMC7189656/ /pubmed/31936719 http://dx.doi.org/10.3390/mps3010005 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Banerjee, Tirtha Das
Monteiro, Antónia
Dissection of Larval and Pupal Wings of Bicyclus anynana Butterflies
title Dissection of Larval and Pupal Wings of Bicyclus anynana Butterflies
title_full Dissection of Larval and Pupal Wings of Bicyclus anynana Butterflies
title_fullStr Dissection of Larval and Pupal Wings of Bicyclus anynana Butterflies
title_full_unstemmed Dissection of Larval and Pupal Wings of Bicyclus anynana Butterflies
title_short Dissection of Larval and Pupal Wings of Bicyclus anynana Butterflies
title_sort dissection of larval and pupal wings of bicyclus anynana butterflies
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189656/
https://www.ncbi.nlm.nih.gov/pubmed/31936719
http://dx.doi.org/10.3390/mps3010005
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