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Dissection of Larval and Pupal Wings of Bicyclus anynana Butterflies
The colorful wings of butterflies are emerging as model systems for evolutionary and developmental studies. Some of these studies focus on localizing gene transcripts and proteins in wings at the larval and pupal stages using techniques such as immunostaining and in situ hybridization. Other studies...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189656/ https://www.ncbi.nlm.nih.gov/pubmed/31936719 http://dx.doi.org/10.3390/mps3010005 |
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author | Banerjee, Tirtha Das Monteiro, Antónia |
author_facet | Banerjee, Tirtha Das Monteiro, Antónia |
author_sort | Banerjee, Tirtha Das |
collection | PubMed |
description | The colorful wings of butterflies are emerging as model systems for evolutionary and developmental studies. Some of these studies focus on localizing gene transcripts and proteins in wings at the larval and pupal stages using techniques such as immunostaining and in situ hybridization. Other studies quantify mRNA expression levels or identify regions of open chromatin that are bound by proteins at different stages of wing development. All these techniques require dissection of the wings from the animal but a detailed video protocol describing this procedure has not been available until now. Here, we present a written and accompanying video protocol where we describe the tools and the method we use to remove the larval and pupal wings of the African Squinting Bush Brown butterfly Bicyclus anynana. This protocol should be easy to adapt to other species. |
format | Online Article Text |
id | pubmed-7189656 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71896562020-05-01 Dissection of Larval and Pupal Wings of Bicyclus anynana Butterflies Banerjee, Tirtha Das Monteiro, Antónia Methods Protoc Protocol The colorful wings of butterflies are emerging as model systems for evolutionary and developmental studies. Some of these studies focus on localizing gene transcripts and proteins in wings at the larval and pupal stages using techniques such as immunostaining and in situ hybridization. Other studies quantify mRNA expression levels or identify regions of open chromatin that are bound by proteins at different stages of wing development. All these techniques require dissection of the wings from the animal but a detailed video protocol describing this procedure has not been available until now. Here, we present a written and accompanying video protocol where we describe the tools and the method we use to remove the larval and pupal wings of the African Squinting Bush Brown butterfly Bicyclus anynana. This protocol should be easy to adapt to other species. MDPI 2020-01-10 /pmc/articles/PMC7189656/ /pubmed/31936719 http://dx.doi.org/10.3390/mps3010005 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Protocol Banerjee, Tirtha Das Monteiro, Antónia Dissection of Larval and Pupal Wings of Bicyclus anynana Butterflies |
title | Dissection of Larval and Pupal Wings of Bicyclus anynana Butterflies |
title_full | Dissection of Larval and Pupal Wings of Bicyclus anynana Butterflies |
title_fullStr | Dissection of Larval and Pupal Wings of Bicyclus anynana Butterflies |
title_full_unstemmed | Dissection of Larval and Pupal Wings of Bicyclus anynana Butterflies |
title_short | Dissection of Larval and Pupal Wings of Bicyclus anynana Butterflies |
title_sort | dissection of larval and pupal wings of bicyclus anynana butterflies |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189656/ https://www.ncbi.nlm.nih.gov/pubmed/31936719 http://dx.doi.org/10.3390/mps3010005 |
work_keys_str_mv | AT banerjeetirthadas dissectionoflarvalandpupalwingsofbicyclusanynanabutterflies AT monteiroantonia dissectionoflarvalandpupalwingsofbicyclusanynanabutterflies |