A Rapid and Accurate Bioluminescence-Based Migration Assay Permitting Analysis of Tumor Cell/Stromal Cell Interactions

Bioluminescent tumor cell lines are used extensively in vivo to monitor tumor growth and metastasis but rarely used in vitro to follow tumor cell behavior. Tumor cell migration is frequently studied in vitro using transwell assays, however, current methods do not permit the co-incubation of tumor ce...

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Detalles Bibliográficos
Autores principales: Yoon, Jinsoo, Parish, Christopher R., Coupland, Lucy A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189659/
https://www.ncbi.nlm.nih.gov/pubmed/31979362
http://dx.doi.org/10.3390/mps3010010
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author Yoon, Jinsoo
Parish, Christopher R.
Coupland, Lucy A.
author_facet Yoon, Jinsoo
Parish, Christopher R.
Coupland, Lucy A.
author_sort Yoon, Jinsoo
collection PubMed
description Bioluminescent tumor cell lines are used extensively in vivo to monitor tumor growth and metastasis but rarely used in vitro to follow tumor cell behavior. Tumor cell migration is frequently studied in vitro using transwell assays, however, current methods do not permit the co-incubation of tumor cells with different stromal cell types for analysis of the effects of intercellular cross-talk on tumor cell migration. We describe a novel migration assay using bioluminescent tumor cell lines that is rapid, accurate, and permits the study of the effects of tumor cell-stromal cell interactions on tumor cell migratory behavior.
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spelling pubmed-71896592020-05-01 A Rapid and Accurate Bioluminescence-Based Migration Assay Permitting Analysis of Tumor Cell/Stromal Cell Interactions Yoon, Jinsoo Parish, Christopher R. Coupland, Lucy A. Methods Protoc Protocol Bioluminescent tumor cell lines are used extensively in vivo to monitor tumor growth and metastasis but rarely used in vitro to follow tumor cell behavior. Tumor cell migration is frequently studied in vitro using transwell assays, however, current methods do not permit the co-incubation of tumor cells with different stromal cell types for analysis of the effects of intercellular cross-talk on tumor cell migration. We describe a novel migration assay using bioluminescent tumor cell lines that is rapid, accurate, and permits the study of the effects of tumor cell-stromal cell interactions on tumor cell migratory behavior. MDPI 2020-01-23 /pmc/articles/PMC7189659/ /pubmed/31979362 http://dx.doi.org/10.3390/mps3010010 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Yoon, Jinsoo
Parish, Christopher R.
Coupland, Lucy A.
A Rapid and Accurate Bioluminescence-Based Migration Assay Permitting Analysis of Tumor Cell/Stromal Cell Interactions
title A Rapid and Accurate Bioluminescence-Based Migration Assay Permitting Analysis of Tumor Cell/Stromal Cell Interactions
title_full A Rapid and Accurate Bioluminescence-Based Migration Assay Permitting Analysis of Tumor Cell/Stromal Cell Interactions
title_fullStr A Rapid and Accurate Bioluminescence-Based Migration Assay Permitting Analysis of Tumor Cell/Stromal Cell Interactions
title_full_unstemmed A Rapid and Accurate Bioluminescence-Based Migration Assay Permitting Analysis of Tumor Cell/Stromal Cell Interactions
title_short A Rapid and Accurate Bioluminescence-Based Migration Assay Permitting Analysis of Tumor Cell/Stromal Cell Interactions
title_sort rapid and accurate bioluminescence-based migration assay permitting analysis of tumor cell/stromal cell interactions
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189659/
https://www.ncbi.nlm.nih.gov/pubmed/31979362
http://dx.doi.org/10.3390/mps3010010
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