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Using a Model of Germ-Free Animals to Study the Impact of Gut Microbiome in Research: A Step by Step Sterility Setting and Management

The particularly unique composition of the gut microbiota has the potential to influence the health or disease status of animal and human hosts. Altering the homeostasis of the host-bacteria could lead to changes in gut flora that result in disease or activation of a specific immunological response,...

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Autores principales: Gabay, Odile, Vicenty, Jonathan, Smith, Dylan, Tiffany, Linda, Ascher, Jill, Curry, Tina, Dennis, John, Clouse, Kathleen A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189661/
https://www.ncbi.nlm.nih.gov/pubmed/32098421
http://dx.doi.org/10.3390/mps3010018
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author Gabay, Odile
Vicenty, Jonathan
Smith, Dylan
Tiffany, Linda
Ascher, Jill
Curry, Tina
Dennis, John
Clouse, Kathleen A.
author_facet Gabay, Odile
Vicenty, Jonathan
Smith, Dylan
Tiffany, Linda
Ascher, Jill
Curry, Tina
Dennis, John
Clouse, Kathleen A.
author_sort Gabay, Odile
collection PubMed
description The particularly unique composition of the gut microbiota has the potential to influence the health or disease status of animal and human hosts. Altering the homeostasis of the host-bacteria could lead to changes in gut flora that result in disease or activation of a specific immunological response, which could explain the variations observed in patient responses to current therapies. A standardized model is crucial for studying the influence of the gut microbiota on therapeutic modalities. A step by step mouse model and sterility management system that compares a control strain of C57BL/6 mice to the established C57BL/6 germ-free (GF) strain has been developed. The GF BL/6 mouse phenotype is well established, and the anatomical differences between the GF and control mice were evident in this model. This method could be applied to research studies investigating the microbiome impact, the response to various therapies, or disease transfer via fecal transplants. A standardized sterility maintenance method is crucial in this context.
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spelling pubmed-71896612020-05-01 Using a Model of Germ-Free Animals to Study the Impact of Gut Microbiome in Research: A Step by Step Sterility Setting and Management Gabay, Odile Vicenty, Jonathan Smith, Dylan Tiffany, Linda Ascher, Jill Curry, Tina Dennis, John Clouse, Kathleen A. Methods Protoc Protocol The particularly unique composition of the gut microbiota has the potential to influence the health or disease status of animal and human hosts. Altering the homeostasis of the host-bacteria could lead to changes in gut flora that result in disease or activation of a specific immunological response, which could explain the variations observed in patient responses to current therapies. A standardized model is crucial for studying the influence of the gut microbiota on therapeutic modalities. A step by step mouse model and sterility management system that compares a control strain of C57BL/6 mice to the established C57BL/6 germ-free (GF) strain has been developed. The GF BL/6 mouse phenotype is well established, and the anatomical differences between the GF and control mice were evident in this model. This method could be applied to research studies investigating the microbiome impact, the response to various therapies, or disease transfer via fecal transplants. A standardized sterility maintenance method is crucial in this context. MDPI 2020-02-22 /pmc/articles/PMC7189661/ /pubmed/32098421 http://dx.doi.org/10.3390/mps3010018 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Gabay, Odile
Vicenty, Jonathan
Smith, Dylan
Tiffany, Linda
Ascher, Jill
Curry, Tina
Dennis, John
Clouse, Kathleen A.
Using a Model of Germ-Free Animals to Study the Impact of Gut Microbiome in Research: A Step by Step Sterility Setting and Management
title Using a Model of Germ-Free Animals to Study the Impact of Gut Microbiome in Research: A Step by Step Sterility Setting and Management
title_full Using a Model of Germ-Free Animals to Study the Impact of Gut Microbiome in Research: A Step by Step Sterility Setting and Management
title_fullStr Using a Model of Germ-Free Animals to Study the Impact of Gut Microbiome in Research: A Step by Step Sterility Setting and Management
title_full_unstemmed Using a Model of Germ-Free Animals to Study the Impact of Gut Microbiome in Research: A Step by Step Sterility Setting and Management
title_short Using a Model of Germ-Free Animals to Study the Impact of Gut Microbiome in Research: A Step by Step Sterility Setting and Management
title_sort using a model of germ-free animals to study the impact of gut microbiome in research: a step by step sterility setting and management
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189661/
https://www.ncbi.nlm.nih.gov/pubmed/32098421
http://dx.doi.org/10.3390/mps3010018
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