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Crewmember microbiome may influence microbial composition of ISS habitable surfaces
The International Space Station (ISS) is a complex built environment physically isolated from Earth. Assessing the interplay between the microbial community of the ISS and its crew is important for preventing biomedical and structural complications for long term human spaceflight missions. In this s...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7190111/ https://www.ncbi.nlm.nih.gov/pubmed/32348348 http://dx.doi.org/10.1371/journal.pone.0231838 |
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author | Avila-Herrera, Aram Thissen, James Urbaniak, Camilla Be, Nicholas A. Smith, David J. Karouia, Fathi Mehta, Satish Venkateswaran, Kasthuri Jaing, Crystal |
author_facet | Avila-Herrera, Aram Thissen, James Urbaniak, Camilla Be, Nicholas A. Smith, David J. Karouia, Fathi Mehta, Satish Venkateswaran, Kasthuri Jaing, Crystal |
author_sort | Avila-Herrera, Aram |
collection | PubMed |
description | The International Space Station (ISS) is a complex built environment physically isolated from Earth. Assessing the interplay between the microbial community of the ISS and its crew is important for preventing biomedical and structural complications for long term human spaceflight missions. In this study, we describe one crewmember’s microbial profile from body swabs of mouth, nose, ear, skin and saliva that were collected at eight different time points pre-, during and post-flight. Additionally, environmental surface samples from eight different habitable locations in the ISS were collected from two flights. Environmental samples from one flight were collected by the crewmember and samples from the next flight were collected after the crewmember departed. The microbial composition in both environment and crewmember samples was measured using shotgun metagenomic sequencing and processed using the Livermore Metagenomics Analysis Toolkit. Ordination of sample to sample distances showed that of the eight crew body sites analyzed, skin, nostril, and ear samples are more similar in microbial composition to the ISS surfaces than mouth and saliva samples; and that the microbial composition of the crewmember’s skin samples are more closely related to the ISS surface samples collected by the crewmember on the same flight than ISS surface samples collected by other crewmembers on different flights. In these collections, species alpha diversity in saliva samples appears to decrease during flight and rebound after returning to Earth. This is the first study to compare the ISS microbiome to a crewmember’s microbiome via shotgun metagenomic sequencing. We observed that the microbiome of the surfaces inside the ISS resemble those of the crew’s skin. These data support future crew and ISS microbial surveillance efforts and the design of preventive measures to maintain crew habitat onboard spacecraft destined for long term space travel. |
format | Online Article Text |
id | pubmed-7190111 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-71901112020-05-06 Crewmember microbiome may influence microbial composition of ISS habitable surfaces Avila-Herrera, Aram Thissen, James Urbaniak, Camilla Be, Nicholas A. Smith, David J. Karouia, Fathi Mehta, Satish Venkateswaran, Kasthuri Jaing, Crystal PLoS One Research Article The International Space Station (ISS) is a complex built environment physically isolated from Earth. Assessing the interplay between the microbial community of the ISS and its crew is important for preventing biomedical and structural complications for long term human spaceflight missions. In this study, we describe one crewmember’s microbial profile from body swabs of mouth, nose, ear, skin and saliva that were collected at eight different time points pre-, during and post-flight. Additionally, environmental surface samples from eight different habitable locations in the ISS were collected from two flights. Environmental samples from one flight were collected by the crewmember and samples from the next flight were collected after the crewmember departed. The microbial composition in both environment and crewmember samples was measured using shotgun metagenomic sequencing and processed using the Livermore Metagenomics Analysis Toolkit. Ordination of sample to sample distances showed that of the eight crew body sites analyzed, skin, nostril, and ear samples are more similar in microbial composition to the ISS surfaces than mouth and saliva samples; and that the microbial composition of the crewmember’s skin samples are more closely related to the ISS surface samples collected by the crewmember on the same flight than ISS surface samples collected by other crewmembers on different flights. In these collections, species alpha diversity in saliva samples appears to decrease during flight and rebound after returning to Earth. This is the first study to compare the ISS microbiome to a crewmember’s microbiome via shotgun metagenomic sequencing. We observed that the microbiome of the surfaces inside the ISS resemble those of the crew’s skin. These data support future crew and ISS microbial surveillance efforts and the design of preventive measures to maintain crew habitat onboard spacecraft destined for long term space travel. Public Library of Science 2020-04-29 /pmc/articles/PMC7190111/ /pubmed/32348348 http://dx.doi.org/10.1371/journal.pone.0231838 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Avila-Herrera, Aram Thissen, James Urbaniak, Camilla Be, Nicholas A. Smith, David J. Karouia, Fathi Mehta, Satish Venkateswaran, Kasthuri Jaing, Crystal Crewmember microbiome may influence microbial composition of ISS habitable surfaces |
title | Crewmember microbiome may influence microbial composition of ISS habitable surfaces |
title_full | Crewmember microbiome may influence microbial composition of ISS habitable surfaces |
title_fullStr | Crewmember microbiome may influence microbial composition of ISS habitable surfaces |
title_full_unstemmed | Crewmember microbiome may influence microbial composition of ISS habitable surfaces |
title_short | Crewmember microbiome may influence microbial composition of ISS habitable surfaces |
title_sort | crewmember microbiome may influence microbial composition of iss habitable surfaces |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7190111/ https://www.ncbi.nlm.nih.gov/pubmed/32348348 http://dx.doi.org/10.1371/journal.pone.0231838 |
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