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Zebrafish embryonic explants undergo genetically encoded self-assembly
Embryonic stem cell cultures are thought to self-organize into embryoid bodies, able to undergo symmetry-breaking, germ layer specification and even morphogenesis. Yet, it is unclear how to reconcile this remarkable self-organization capacity with classical experiments demonstrating key roles for ex...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7190352/ https://www.ncbi.nlm.nih.gov/pubmed/32250246 http://dx.doi.org/10.7554/eLife.55190 |
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author | Schauer, Alexandra Pinheiro, Diana Hauschild, Robert Heisenberg, Carl-Philipp |
author_facet | Schauer, Alexandra Pinheiro, Diana Hauschild, Robert Heisenberg, Carl-Philipp |
author_sort | Schauer, Alexandra |
collection | PubMed |
description | Embryonic stem cell cultures are thought to self-organize into embryoid bodies, able to undergo symmetry-breaking, germ layer specification and even morphogenesis. Yet, it is unclear how to reconcile this remarkable self-organization capacity with classical experiments demonstrating key roles for extrinsic biases by maternal factors and/or extraembryonic tissues in embryogenesis. Here, we show that zebrafish embryonic tissue explants, prepared prior to germ layer induction and lacking extraembryonic tissues, can specify all germ layers and form a seemingly complete mesendoderm anlage. Importantly, explant organization requires polarized inheritance of maternal factors from dorsal-marginal regions of the blastoderm. Moreover, induction of endoderm and head-mesoderm, which require peak Nodal-signaling levels, is highly variable in explants, reminiscent of embryos with reduced Nodal signals from the extraembryonic tissues. Together, these data suggest that zebrafish explants do not undergo bona fide self-organization, but rather display features of genetically encoded self-assembly, where intrinsic genetic programs control the emergence of order. |
format | Online Article Text |
id | pubmed-7190352 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-71903522020-05-01 Zebrafish embryonic explants undergo genetically encoded self-assembly Schauer, Alexandra Pinheiro, Diana Hauschild, Robert Heisenberg, Carl-Philipp eLife Developmental Biology Embryonic stem cell cultures are thought to self-organize into embryoid bodies, able to undergo symmetry-breaking, germ layer specification and even morphogenesis. Yet, it is unclear how to reconcile this remarkable self-organization capacity with classical experiments demonstrating key roles for extrinsic biases by maternal factors and/or extraembryonic tissues in embryogenesis. Here, we show that zebrafish embryonic tissue explants, prepared prior to germ layer induction and lacking extraembryonic tissues, can specify all germ layers and form a seemingly complete mesendoderm anlage. Importantly, explant organization requires polarized inheritance of maternal factors from dorsal-marginal regions of the blastoderm. Moreover, induction of endoderm and head-mesoderm, which require peak Nodal-signaling levels, is highly variable in explants, reminiscent of embryos with reduced Nodal signals from the extraembryonic tissues. Together, these data suggest that zebrafish explants do not undergo bona fide self-organization, but rather display features of genetically encoded self-assembly, where intrinsic genetic programs control the emergence of order. eLife Sciences Publications, Ltd 2020-04-06 /pmc/articles/PMC7190352/ /pubmed/32250246 http://dx.doi.org/10.7554/eLife.55190 Text en © 2020, Schauer et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology Schauer, Alexandra Pinheiro, Diana Hauschild, Robert Heisenberg, Carl-Philipp Zebrafish embryonic explants undergo genetically encoded self-assembly |
title | Zebrafish embryonic explants undergo genetically encoded self-assembly |
title_full | Zebrafish embryonic explants undergo genetically encoded self-assembly |
title_fullStr | Zebrafish embryonic explants undergo genetically encoded self-assembly |
title_full_unstemmed | Zebrafish embryonic explants undergo genetically encoded self-assembly |
title_short | Zebrafish embryonic explants undergo genetically encoded self-assembly |
title_sort | zebrafish embryonic explants undergo genetically encoded self-assembly |
topic | Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7190352/ https://www.ncbi.nlm.nih.gov/pubmed/32250246 http://dx.doi.org/10.7554/eLife.55190 |
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