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Effect of Mesenchymal Stem Cell-derived Microvesicles on Megakaryocytic Differentiation of CD34(+) Hematopoietic Stem Cells

Purpose: Mesenchymal stem cells (MSCs) release hematopoietic cytokines, growth factors, and Microvesicles (MVs) supporting the hematopoietic stem cells (HSCs). MVs released from various cells, playing a crucial role in biological functions of their parental cells. MSC-derived MVs contain microRNAs a...

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Autores principales: Aqmasheh, Sara, Shamsasenjan, Karim, Khalaf Adeli, Elham, Movassaghpourakbari, Aliakbar, Akbarzadehlaleh, Parvin, Pashoutan Sarvar, Davod, Timari, Hamzeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tabriz University of Medical Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191234/
https://www.ncbi.nlm.nih.gov/pubmed/32373502
http://dx.doi.org/10.34172/apb.2020.038
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author Aqmasheh, Sara
Shamsasenjan, Karim
Khalaf Adeli, Elham
Movassaghpourakbari, Aliakbar
Akbarzadehlaleh, Parvin
Pashoutan Sarvar, Davod
Timari, Hamzeh
author_facet Aqmasheh, Sara
Shamsasenjan, Karim
Khalaf Adeli, Elham
Movassaghpourakbari, Aliakbar
Akbarzadehlaleh, Parvin
Pashoutan Sarvar, Davod
Timari, Hamzeh
author_sort Aqmasheh, Sara
collection PubMed
description Purpose: Mesenchymal stem cells (MSCs) release hematopoietic cytokines, growth factors, and Microvesicles (MVs) supporting the hematopoietic stem cells (HSCs). MVs released from various cells, playing a crucial role in biological functions of their parental cells. MSC-derived MVs contain microRNAs and proteins with key roles in the regulation of hematopoiesis. Umbilical cord blood (UCB) is a source for transplantation but the long-term recovery of platelets is a main problem. Therefore, we intend to show that MSC-MVs are able to improve the differentiation of UCB-derived CD34(+) cells to megakaryocyte lineage. Methods: In this descriptive study, MSCs were cultured in DMEM to collect the culture supernatant, which was ultracentrifuged for the isolation of MVs. HSCs were isolated from UCB using MACS method and cultured in IMDM supplemented with cytokines and MVs in three different conditions. Megakaryocyte differentiation was evaluated through the expression of specific markers and genes after 72 hours, and the data was analyzed by t test (P<0.05). Results: The expression of specific megakaryocyte markers (CD41 and CD61) in the presence of different concentrations of MSC-MVs did not show any significant difference. Also, the expression of specific genes of megakaryocyte lineage was compared with control group. The expression of GATA2 and c-Mpl was significantly increased, GATA1 was not significantly decreased, and FLI1 was significantly decreased. Conclusion: MSC-MVs could improve the expression of specific megakaryocyte genes; however, there was no significant expression of CD markers. Further studies, including the evaluation of late stages of megakaryocyte differentiation, are required to evaluate platelet production and shedding
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spelling pubmed-71912342020-05-05 Effect of Mesenchymal Stem Cell-derived Microvesicles on Megakaryocytic Differentiation of CD34(+) Hematopoietic Stem Cells Aqmasheh, Sara Shamsasenjan, Karim Khalaf Adeli, Elham Movassaghpourakbari, Aliakbar Akbarzadehlaleh, Parvin Pashoutan Sarvar, Davod Timari, Hamzeh Adv Pharm Bull Research Article Purpose: Mesenchymal stem cells (MSCs) release hematopoietic cytokines, growth factors, and Microvesicles (MVs) supporting the hematopoietic stem cells (HSCs). MVs released from various cells, playing a crucial role in biological functions of their parental cells. MSC-derived MVs contain microRNAs and proteins with key roles in the regulation of hematopoiesis. Umbilical cord blood (UCB) is a source for transplantation but the long-term recovery of platelets is a main problem. Therefore, we intend to show that MSC-MVs are able to improve the differentiation of UCB-derived CD34(+) cells to megakaryocyte lineage. Methods: In this descriptive study, MSCs were cultured in DMEM to collect the culture supernatant, which was ultracentrifuged for the isolation of MVs. HSCs were isolated from UCB using MACS method and cultured in IMDM supplemented with cytokines and MVs in three different conditions. Megakaryocyte differentiation was evaluated through the expression of specific markers and genes after 72 hours, and the data was analyzed by t test (P<0.05). Results: The expression of specific megakaryocyte markers (CD41 and CD61) in the presence of different concentrations of MSC-MVs did not show any significant difference. Also, the expression of specific genes of megakaryocyte lineage was compared with control group. The expression of GATA2 and c-Mpl was significantly increased, GATA1 was not significantly decreased, and FLI1 was significantly decreased. Conclusion: MSC-MVs could improve the expression of specific megakaryocyte genes; however, there was no significant expression of CD markers. Further studies, including the evaluation of late stages of megakaryocyte differentiation, are required to evaluate platelet production and shedding Tabriz University of Medical Sciences 2020-06 2020-02-20 /pmc/articles/PMC7191234/ /pubmed/32373502 http://dx.doi.org/10.34172/apb.2020.038 Text en © 2020 The Authors. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, as long as the original authors and source are cited. No permission is required from the authors or the publishers.
spellingShingle Research Article
Aqmasheh, Sara
Shamsasenjan, Karim
Khalaf Adeli, Elham
Movassaghpourakbari, Aliakbar
Akbarzadehlaleh, Parvin
Pashoutan Sarvar, Davod
Timari, Hamzeh
Effect of Mesenchymal Stem Cell-derived Microvesicles on Megakaryocytic Differentiation of CD34(+) Hematopoietic Stem Cells
title Effect of Mesenchymal Stem Cell-derived Microvesicles on Megakaryocytic Differentiation of CD34(+) Hematopoietic Stem Cells
title_full Effect of Mesenchymal Stem Cell-derived Microvesicles on Megakaryocytic Differentiation of CD34(+) Hematopoietic Stem Cells
title_fullStr Effect of Mesenchymal Stem Cell-derived Microvesicles on Megakaryocytic Differentiation of CD34(+) Hematopoietic Stem Cells
title_full_unstemmed Effect of Mesenchymal Stem Cell-derived Microvesicles on Megakaryocytic Differentiation of CD34(+) Hematopoietic Stem Cells
title_short Effect of Mesenchymal Stem Cell-derived Microvesicles on Megakaryocytic Differentiation of CD34(+) Hematopoietic Stem Cells
title_sort effect of mesenchymal stem cell-derived microvesicles on megakaryocytic differentiation of cd34(+) hematopoietic stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191234/
https://www.ncbi.nlm.nih.gov/pubmed/32373502
http://dx.doi.org/10.34172/apb.2020.038
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