Draft genome sequences of Hirudo medicinalis and salivary transcriptome of three closely related medicinal leeches

BACKGROUND: Salivary cell secretion (SCS) plays a critical role in blood feeding by medicinal leeches, making them of use for certain medical purposes even today. RESULTS: We annotated the Hirudo medicinalis genome and performed RNA-seq on salivary cells isolated from three closely related leech spe...

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Autores principales: Babenko, Vladislav V., Podgorny, Oleg V., Manuvera, Valentin A., Kasianov, Artem S., Manolov, Alexander I., Grafskaia, Ekaterina N., Shirokov, Dmitriy A., Kurdyumov, Alexey S., Vinogradov, Dmitriy V., Nikitina, Anastasia S., Kovalchuk, Sergey I., Anikanov, Nickolay A., Butenko, Ivan O., Pobeguts, Olga V., Matyushkina, Daria S., Rakitina, Daria V., Kostryukova, Elena S., Zgoda, Victor G., Baskova, Isolda P., Trukhan, Vladimir M., Gelfand, Mikhail S., Govorun, Vadim M., Schiöth, Helgi B., Lazarev, Vassili N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191736/
https://www.ncbi.nlm.nih.gov/pubmed/32349672
http://dx.doi.org/10.1186/s12864-020-6748-0
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author Babenko, Vladislav V.
Podgorny, Oleg V.
Manuvera, Valentin A.
Kasianov, Artem S.
Manolov, Alexander I.
Grafskaia, Ekaterina N.
Shirokov, Dmitriy A.
Kurdyumov, Alexey S.
Vinogradov, Dmitriy V.
Nikitina, Anastasia S.
Kovalchuk, Sergey I.
Anikanov, Nickolay A.
Butenko, Ivan O.
Pobeguts, Olga V.
Matyushkina, Daria S.
Rakitina, Daria V.
Kostryukova, Elena S.
Zgoda, Victor G.
Baskova, Isolda P.
Trukhan, Vladimir M.
Gelfand, Mikhail S.
Govorun, Vadim M.
Schiöth, Helgi B.
Lazarev, Vassili N.
author_facet Babenko, Vladislav V.
Podgorny, Oleg V.
Manuvera, Valentin A.
Kasianov, Artem S.
Manolov, Alexander I.
Grafskaia, Ekaterina N.
Shirokov, Dmitriy A.
Kurdyumov, Alexey S.
Vinogradov, Dmitriy V.
Nikitina, Anastasia S.
Kovalchuk, Sergey I.
Anikanov, Nickolay A.
Butenko, Ivan O.
Pobeguts, Olga V.
Matyushkina, Daria S.
Rakitina, Daria V.
Kostryukova, Elena S.
Zgoda, Victor G.
Baskova, Isolda P.
Trukhan, Vladimir M.
Gelfand, Mikhail S.
Govorun, Vadim M.
Schiöth, Helgi B.
Lazarev, Vassili N.
author_sort Babenko, Vladislav V.
collection PubMed
description BACKGROUND: Salivary cell secretion (SCS) plays a critical role in blood feeding by medicinal leeches, making them of use for certain medical purposes even today. RESULTS: We annotated the Hirudo medicinalis genome and performed RNA-seq on salivary cells isolated from three closely related leech species, H. medicinalis, Hirudo orientalis, and Hirudo verbana. Differential expression analysis verified by proteomics identified salivary cell-specific gene expression, many of which encode previously unknown salivary components. However, the genes encoding known anticoagulants have been found to be expressed not only in salivary cells. The function-related analysis of the unique salivary cell genes enabled an update of the concept of interactions between salivary proteins and components of haemostasis. CONCLUSIONS: Here we report a genome draft of Hirudo medicinalis and describe identification of novel salivary proteins and new homologs of genes encoding known anticoagulants in transcriptomes of three medicinal leech species. Our data provide new insights in genetics of blood-feeding lifestyle in leeches.
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spelling pubmed-71917362020-05-04 Draft genome sequences of Hirudo medicinalis and salivary transcriptome of three closely related medicinal leeches Babenko, Vladislav V. Podgorny, Oleg V. Manuvera, Valentin A. Kasianov, Artem S. Manolov, Alexander I. Grafskaia, Ekaterina N. Shirokov, Dmitriy A. Kurdyumov, Alexey S. Vinogradov, Dmitriy V. Nikitina, Anastasia S. Kovalchuk, Sergey I. Anikanov, Nickolay A. Butenko, Ivan O. Pobeguts, Olga V. Matyushkina, Daria S. Rakitina, Daria V. Kostryukova, Elena S. Zgoda, Victor G. Baskova, Isolda P. Trukhan, Vladimir M. Gelfand, Mikhail S. Govorun, Vadim M. Schiöth, Helgi B. Lazarev, Vassili N. BMC Genomics Research Article BACKGROUND: Salivary cell secretion (SCS) plays a critical role in blood feeding by medicinal leeches, making them of use for certain medical purposes even today. RESULTS: We annotated the Hirudo medicinalis genome and performed RNA-seq on salivary cells isolated from three closely related leech species, H. medicinalis, Hirudo orientalis, and Hirudo verbana. Differential expression analysis verified by proteomics identified salivary cell-specific gene expression, many of which encode previously unknown salivary components. However, the genes encoding known anticoagulants have been found to be expressed not only in salivary cells. The function-related analysis of the unique salivary cell genes enabled an update of the concept of interactions between salivary proteins and components of haemostasis. CONCLUSIONS: Here we report a genome draft of Hirudo medicinalis and describe identification of novel salivary proteins and new homologs of genes encoding known anticoagulants in transcriptomes of three medicinal leech species. Our data provide new insights in genetics of blood-feeding lifestyle in leeches. BioMed Central 2020-04-29 /pmc/articles/PMC7191736/ /pubmed/32349672 http://dx.doi.org/10.1186/s12864-020-6748-0 Text en © The Author(s). 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Babenko, Vladislav V.
Podgorny, Oleg V.
Manuvera, Valentin A.
Kasianov, Artem S.
Manolov, Alexander I.
Grafskaia, Ekaterina N.
Shirokov, Dmitriy A.
Kurdyumov, Alexey S.
Vinogradov, Dmitriy V.
Nikitina, Anastasia S.
Kovalchuk, Sergey I.
Anikanov, Nickolay A.
Butenko, Ivan O.
Pobeguts, Olga V.
Matyushkina, Daria S.
Rakitina, Daria V.
Kostryukova, Elena S.
Zgoda, Victor G.
Baskova, Isolda P.
Trukhan, Vladimir M.
Gelfand, Mikhail S.
Govorun, Vadim M.
Schiöth, Helgi B.
Lazarev, Vassili N.
Draft genome sequences of Hirudo medicinalis and salivary transcriptome of three closely related medicinal leeches
title Draft genome sequences of Hirudo medicinalis and salivary transcriptome of three closely related medicinal leeches
title_full Draft genome sequences of Hirudo medicinalis and salivary transcriptome of three closely related medicinal leeches
title_fullStr Draft genome sequences of Hirudo medicinalis and salivary transcriptome of three closely related medicinal leeches
title_full_unstemmed Draft genome sequences of Hirudo medicinalis and salivary transcriptome of three closely related medicinal leeches
title_short Draft genome sequences of Hirudo medicinalis and salivary transcriptome of three closely related medicinal leeches
title_sort draft genome sequences of hirudo medicinalis and salivary transcriptome of three closely related medicinal leeches
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191736/
https://www.ncbi.nlm.nih.gov/pubmed/32349672
http://dx.doi.org/10.1186/s12864-020-6748-0
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