Cargando…

Roles of eIF3m in the tumorigenesis of triple negative breast cancer

BACKGROUND: Without targets, triple negative breast cancer (TNBC) has the worst prognosis in all subtypes of breast cancer (BC). Recently, eukaryotic translation initiation factor 3 m (eIF3m) has been declared to be involved in the malignant progression of various neoplasms. The aim of this study is...

Descripción completa

Detalles Bibliográficos
Autores principales: Han, Wei, Zhang, Cong, Shi, Chun-tao, Gao, Xiao-jiao, Zhou, Ming-hui, Shao, Qi-xiang, Shen, Xiao-jun, Wu, Cheng-jiang, Cao, Fang, Hu, Yong-wei, Yuan, Jian-liang, Ding, Hou-zhong, Wang, Qing-hua, Wang, Hao-nan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191806/
https://www.ncbi.nlm.nih.gov/pubmed/32368187
http://dx.doi.org/10.1186/s12935-020-01220-z
_version_ 1783527918011416576
author Han, Wei
Zhang, Cong
Shi, Chun-tao
Gao, Xiao-jiao
Zhou, Ming-hui
Shao, Qi-xiang
Shen, Xiao-jun
Wu, Cheng-jiang
Cao, Fang
Hu, Yong-wei
Yuan, Jian-liang
Ding, Hou-zhong
Wang, Qing-hua
Wang, Hao-nan
author_facet Han, Wei
Zhang, Cong
Shi, Chun-tao
Gao, Xiao-jiao
Zhou, Ming-hui
Shao, Qi-xiang
Shen, Xiao-jun
Wu, Cheng-jiang
Cao, Fang
Hu, Yong-wei
Yuan, Jian-liang
Ding, Hou-zhong
Wang, Qing-hua
Wang, Hao-nan
author_sort Han, Wei
collection PubMed
description BACKGROUND: Without targets, triple negative breast cancer (TNBC) has the worst prognosis in all subtypes of breast cancer (BC). Recently, eukaryotic translation initiation factor 3 m (eIF3m) has been declared to be involved in the malignant progression of various neoplasms. The aim of this study is to explore biological functions of eIF3m in TNBC. METHODS: Multiple databases, including Oncomine, KM-plotter and so on, were performed to analyze prognosis and function of eIF3m in TNBC. After transfection of eIF3m-shRNA lentivirus, CCK-8, colony formation assay, cell cycle analysis, wound healing assay, transwell assays, mitochondrial membrane potential assay and cell apoptosis analysis were performed to explore the roles of eIF3m in TNBC cell bio-behaviors. In addition, western blotting was conducted to analyze the potential molecular mechanisms of eIF3m. RESULTS: In multiple databases, up-regulated eIF3m had lower overall survival, relapse-free survival and post progression survival in BC. EIF3m expression in TNBC was obviously higher than in non-TNBC or normal breast tissues. Its expression in TNBC was positively related to differentiation, lymph node invasion and distant metastasis. After knockdown of eIF3m, cell proliferation, migration, invasion and levels of mitochondrial membrane potential of MDA-MB-231 and MDA-MB-436 were all significantly suppressed, while apoptosis rates of them were obviously increased. In addition, eIF3m could regulate cell-cycle, epithelial–mesenchymal transition and apoptosis-related proteins. Combined with public databases and RT-qPCR, 14 genes were identified to be modulated by eIF3m in the development of TNBC. CONCLUSIONS: eIF3m is an unfavorable indicator of TNBC, and plays a vital role in the process of TNBC tumorigenesis.
format Online
Article
Text
id pubmed-7191806
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-71918062020-05-04 Roles of eIF3m in the tumorigenesis of triple negative breast cancer Han, Wei Zhang, Cong Shi, Chun-tao Gao, Xiao-jiao Zhou, Ming-hui Shao, Qi-xiang Shen, Xiao-jun Wu, Cheng-jiang Cao, Fang Hu, Yong-wei Yuan, Jian-liang Ding, Hou-zhong Wang, Qing-hua Wang, Hao-nan Cancer Cell Int Primary Research BACKGROUND: Without targets, triple negative breast cancer (TNBC) has the worst prognosis in all subtypes of breast cancer (BC). Recently, eukaryotic translation initiation factor 3 m (eIF3m) has been declared to be involved in the malignant progression of various neoplasms. The aim of this study is to explore biological functions of eIF3m in TNBC. METHODS: Multiple databases, including Oncomine, KM-plotter and so on, were performed to analyze prognosis and function of eIF3m in TNBC. After transfection of eIF3m-shRNA lentivirus, CCK-8, colony formation assay, cell cycle analysis, wound healing assay, transwell assays, mitochondrial membrane potential assay and cell apoptosis analysis were performed to explore the roles of eIF3m in TNBC cell bio-behaviors. In addition, western blotting was conducted to analyze the potential molecular mechanisms of eIF3m. RESULTS: In multiple databases, up-regulated eIF3m had lower overall survival, relapse-free survival and post progression survival in BC. EIF3m expression in TNBC was obviously higher than in non-TNBC or normal breast tissues. Its expression in TNBC was positively related to differentiation, lymph node invasion and distant metastasis. After knockdown of eIF3m, cell proliferation, migration, invasion and levels of mitochondrial membrane potential of MDA-MB-231 and MDA-MB-436 were all significantly suppressed, while apoptosis rates of them were obviously increased. In addition, eIF3m could regulate cell-cycle, epithelial–mesenchymal transition and apoptosis-related proteins. Combined with public databases and RT-qPCR, 14 genes were identified to be modulated by eIF3m in the development of TNBC. CONCLUSIONS: eIF3m is an unfavorable indicator of TNBC, and plays a vital role in the process of TNBC tumorigenesis. BioMed Central 2020-04-29 /pmc/articles/PMC7191806/ /pubmed/32368187 http://dx.doi.org/10.1186/s12935-020-01220-z Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Primary Research
Han, Wei
Zhang, Cong
Shi, Chun-tao
Gao, Xiao-jiao
Zhou, Ming-hui
Shao, Qi-xiang
Shen, Xiao-jun
Wu, Cheng-jiang
Cao, Fang
Hu, Yong-wei
Yuan, Jian-liang
Ding, Hou-zhong
Wang, Qing-hua
Wang, Hao-nan
Roles of eIF3m in the tumorigenesis of triple negative breast cancer
title Roles of eIF3m in the tumorigenesis of triple negative breast cancer
title_full Roles of eIF3m in the tumorigenesis of triple negative breast cancer
title_fullStr Roles of eIF3m in the tumorigenesis of triple negative breast cancer
title_full_unstemmed Roles of eIF3m in the tumorigenesis of triple negative breast cancer
title_short Roles of eIF3m in the tumorigenesis of triple negative breast cancer
title_sort roles of eif3m in the tumorigenesis of triple negative breast cancer
topic Primary Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191806/
https://www.ncbi.nlm.nih.gov/pubmed/32368187
http://dx.doi.org/10.1186/s12935-020-01220-z
work_keys_str_mv AT hanwei rolesofeif3minthetumorigenesisoftriplenegativebreastcancer
AT zhangcong rolesofeif3minthetumorigenesisoftriplenegativebreastcancer
AT shichuntao rolesofeif3minthetumorigenesisoftriplenegativebreastcancer
AT gaoxiaojiao rolesofeif3minthetumorigenesisoftriplenegativebreastcancer
AT zhouminghui rolesofeif3minthetumorigenesisoftriplenegativebreastcancer
AT shaoqixiang rolesofeif3minthetumorigenesisoftriplenegativebreastcancer
AT shenxiaojun rolesofeif3minthetumorigenesisoftriplenegativebreastcancer
AT wuchengjiang rolesofeif3minthetumorigenesisoftriplenegativebreastcancer
AT caofang rolesofeif3minthetumorigenesisoftriplenegativebreastcancer
AT huyongwei rolesofeif3minthetumorigenesisoftriplenegativebreastcancer
AT yuanjianliang rolesofeif3minthetumorigenesisoftriplenegativebreastcancer
AT dinghouzhong rolesofeif3minthetumorigenesisoftriplenegativebreastcancer
AT wangqinghua rolesofeif3minthetumorigenesisoftriplenegativebreastcancer
AT wanghaonan rolesofeif3minthetumorigenesisoftriplenegativebreastcancer