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Tanshinone IIA protects mice against atherosclerotic injury by activating the TGF-β/PI3K/Akt/eNOS pathway

Explored the mechanism of action of tanshinone IIA (TIIA) against atherosclerosis. METHODS: ApoE(−/−) mice were divided into two groups of 10: model and TIIA. A control group of 10 wild-type mice was created. ApoE(−/−) mice were fed a high-fat diet for 12 weeks. The TIIA group received TIIA once dai...

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Detalles Bibliográficos
Autores principales: Wang, Junyan, He, Xinyong, Chen, Wenna, Zhang, Ni, Guo, Junfu, Liu, Jingjing, Zhang, Lin, Sun, Hongwei, Jia, Lianqun, Yang, Guanlin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7192539/
https://www.ncbi.nlm.nih.gov/pubmed/31842027
http://dx.doi.org/10.1097/MCA.0000000000000835
Descripción
Sumario:Explored the mechanism of action of tanshinone IIA (TIIA) against atherosclerosis. METHODS: ApoE(−/−) mice were divided into two groups of 10: model and TIIA. A control group of 10 wild-type mice was created. ApoE(−/−) mice were fed a high-fat diet for 12 weeks. The TIIA group received TIIA once daily. Mice were anesthetized, blood collected by cardiac puncture, and the aortic sinus/arch collected for histology and molecular studies, respectively. RESULTS: Mice intima in the model group had large areas of plaque formation, serum levels of total cholesterol (TC), triglycerides, and low-density lipoprotein-cholesterol (LDL-C) increased significantly, and high-density lipoprotein-cholesterol (HDL-C) levels decreased significantly in the model group after 12 weeks. Staining [hematoxylin and eosin (H&E), Oil-Red-O] showed that the aorta had lesions, a higher degree of plaque formation, and considerable lipid deposition in model-group mice. After TIIA treatment, expression of HDL-C was increased significantly and that of TC, triglycerides and LDL-C decreased significantly, and plaque size and lipid deposition improved obviously. Analyses of protein phosphorylation in aortic tissue suggested that the transforming growth factor (TGF)-β/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)/endothelial nitric oxide synthase (eNOS) pathway was activated in TIIA-treated mice. CONCLUSION: TIIA can lower levels of serum lipids, stabilize atherosclerotic plaques, reduce endothelial injury, and inflammatory damage by activation of the TGF-β/PI3K/Akt/eNOS pathway.