A GoldenBraid cloning system for synthetic biology in social amoebae

GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria–host interactions, and allorecognition. We present Gol...

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Detalles Bibliográficos
Autores principales: Kundert, Peter, Sarrion-Perdigones, Alejandro, Gonzalez, Yezabel, Katoh-Kurasawa, Mariko, Hirose, Shigenori, Lehmann, Peter, Venken, Koen J T, Shaulsky, Gad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Data Resources and Analyses
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7192589/
https://www.ncbi.nlm.nih.gov/pubmed/32232356
http://dx.doi.org/10.1093/nar/gkaa185
Descripción
Sumario:GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria–host interactions, and allorecognition. We present GoldenBraid as a synthetic biological framework for Dictyostelium, including a library of 250 DNA parts and assemblies and a proof-of-concept strain that illustrates cAMP-chemotaxis with four fluorescent reporters coded by one plasmid.

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