A GoldenBraid cloning system for synthetic biology in social amoebae

GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria–host interactions, and allorecognition. We present Gol...

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Autores principales: Kundert, Peter, Sarrion-Perdigones, Alejandro, Gonzalez, Yezabel, Katoh-Kurasawa, Mariko, Hirose, Shigenori, Lehmann, Peter, Venken, Koen J T, Shaulsky, Gad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Data Resources and Analyses
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7192589/
https://www.ncbi.nlm.nih.gov/pubmed/32232356
http://dx.doi.org/10.1093/nar/gkaa185
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author Kundert, Peter
Sarrion-Perdigones, Alejandro
Gonzalez, Yezabel
Katoh-Kurasawa, Mariko
Hirose, Shigenori
Lehmann, Peter
Venken, Koen J T
Shaulsky, Gad
author_facet Kundert, Peter
Sarrion-Perdigones, Alejandro
Gonzalez, Yezabel
Katoh-Kurasawa, Mariko
Hirose, Shigenori
Lehmann, Peter
Venken, Koen J T
Shaulsky, Gad
author_sort Kundert, Peter
collection PubMed
description GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria–host interactions, and allorecognition. We present GoldenBraid as a synthetic biological framework for Dictyostelium, including a library of 250 DNA parts and assemblies and a proof-of-concept strain that illustrates cAMP-chemotaxis with four fluorescent reporters coded by one plasmid.
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spelling pubmed-71925892020-05-06 A GoldenBraid cloning system for synthetic biology in social amoebae Kundert, Peter Sarrion-Perdigones, Alejandro Gonzalez, Yezabel Katoh-Kurasawa, Mariko Hirose, Shigenori Lehmann, Peter Venken, Koen J T Shaulsky, Gad Nucleic Acids Res Data Resources and Analyses GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria–host interactions, and allorecognition. We present GoldenBraid as a synthetic biological framework for Dictyostelium, including a library of 250 DNA parts and assemblies and a proof-of-concept strain that illustrates cAMP-chemotaxis with four fluorescent reporters coded by one plasmid. Oxford University Press 2020-05-07 2020-03-30 /pmc/articles/PMC7192589/ /pubmed/32232356 http://dx.doi.org/10.1093/nar/gkaa185 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Data Resources and Analyses
Kundert, Peter
Sarrion-Perdigones, Alejandro
Gonzalez, Yezabel
Katoh-Kurasawa, Mariko
Hirose, Shigenori
Lehmann, Peter
Venken, Koen J T
Shaulsky, Gad
A GoldenBraid cloning system for synthetic biology in social amoebae
title A GoldenBraid cloning system for synthetic biology in social amoebae
title_full A GoldenBraid cloning system for synthetic biology in social amoebae
title_fullStr A GoldenBraid cloning system for synthetic biology in social amoebae
title_full_unstemmed A GoldenBraid cloning system for synthetic biology in social amoebae
title_short A GoldenBraid cloning system for synthetic biology in social amoebae
title_sort goldenbraid cloning system for synthetic biology in social amoebae
topic Data Resources and Analyses
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7192589/
https://www.ncbi.nlm.nih.gov/pubmed/32232356
http://dx.doi.org/10.1093/nar/gkaa185
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