Rapid mesoscale volumetric imaging of neural activity with synaptic resolution

Imaging neurons and neural circuits over large volumes at high speed and subcellular resolution is a difficult task. Incorporating a Bessel focus module into a two-photon fluorescence mesoscope, we achieved rapid volumetric imaging of neural activity over mesoscale with synaptic resolution. We appli...

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Detalles Bibliográficos
Autores principales: Lu, Rongwen, Liang, Yajie, Meng, Guanghan, Zhou, Pengcheng, Svoboda, Karel, Paninski, Liam, Ji, Na
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7192636/
https://www.ncbi.nlm.nih.gov/pubmed/32123393
http://dx.doi.org/10.1038/s41592-020-0760-9
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author Lu, Rongwen
Liang, Yajie
Meng, Guanghan
Zhou, Pengcheng
Svoboda, Karel
Paninski, Liam
Ji, Na
author_facet Lu, Rongwen
Liang, Yajie
Meng, Guanghan
Zhou, Pengcheng
Svoboda, Karel
Paninski, Liam
Ji, Na
author_sort Lu, Rongwen
collection PubMed
description Imaging neurons and neural circuits over large volumes at high speed and subcellular resolution is a difficult task. Incorporating a Bessel focus module into a two-photon fluorescence mesoscope, we achieved rapid volumetric imaging of neural activity over mesoscale with synaptic resolution. We applied it to calcium imaging of entire dendritic spans of neurons as well as neural ensembles within multiple cortical regions over two hemispheres of the awake mouse brain.
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spelling pubmed-71926362020-09-02 Rapid mesoscale volumetric imaging of neural activity with synaptic resolution Lu, Rongwen Liang, Yajie Meng, Guanghan Zhou, Pengcheng Svoboda, Karel Paninski, Liam Ji, Na Nat Methods Article Imaging neurons and neural circuits over large volumes at high speed and subcellular resolution is a difficult task. Incorporating a Bessel focus module into a two-photon fluorescence mesoscope, we achieved rapid volumetric imaging of neural activity over mesoscale with synaptic resolution. We applied it to calcium imaging of entire dendritic spans of neurons as well as neural ensembles within multiple cortical regions over two hemispheres of the awake mouse brain. 2020-03-02 2020-03 /pmc/articles/PMC7192636/ /pubmed/32123393 http://dx.doi.org/10.1038/s41592-020-0760-9 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Lu, Rongwen
Liang, Yajie
Meng, Guanghan
Zhou, Pengcheng
Svoboda, Karel
Paninski, Liam
Ji, Na
Rapid mesoscale volumetric imaging of neural activity with synaptic resolution
title Rapid mesoscale volumetric imaging of neural activity with synaptic resolution
title_full Rapid mesoscale volumetric imaging of neural activity with synaptic resolution
title_fullStr Rapid mesoscale volumetric imaging of neural activity with synaptic resolution
title_full_unstemmed Rapid mesoscale volumetric imaging of neural activity with synaptic resolution
title_short Rapid mesoscale volumetric imaging of neural activity with synaptic resolution
title_sort rapid mesoscale volumetric imaging of neural activity with synaptic resolution
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7192636/
https://www.ncbi.nlm.nih.gov/pubmed/32123393
http://dx.doi.org/10.1038/s41592-020-0760-9
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