Cargando…

Sensitive and Rapid Phenotyping of Microbes With Soluble Methane Monooxygenase Using a Droplet-Based Assay

Methanotrophs with soluble methane monooxygenase (sMMO) show high potential for various ecological and biotechnological applications. Here, we developed a high throughput method to identify sMMO-producing microbes by integrating droplet microfluidics and a genetic circuit-based biosensor system. sMM...

Descripción completa

Detalles Bibliográficos
Autores principales:	Lee, Hyewon, Baek, Ji In, Kim, Su Jin, Kwon, Kil Koang, Rha, Eugene, Yeom, Soo-Jin, Kim, Haseong, Lee, Dae-Hee, Kim, Dong-Myung, Lee, Seung-Goo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2020
Materias:	Bioengineering and Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7193049/ https://www.ncbi.nlm.nih.gov/pubmed/32391352 http://dx.doi.org/10.3389/fbioe.2020.00358

Ejemplares similares

Discovery and Biochemical Characterization of a Methanol Dehydrogenase From Lysinibacillus xylanilyticus
por: Lee, Jin-Young, et al.
Publicado: (2020)

CRISPRi-based programmable logic inverter cascade for antibiotic-free selection and maintenance of multiple plasmids
por: Kim, Seong Keun, et al.
Publicado: (2022)

Antagonistic Control of Genetic Circuit Performance for Rapid Analysis of Targeted Enzyme Activity in Living Cells
por: Kwon, Kil Koang, et al.
Publicado: (2021)

Acclimation of bacterial cell state for high-throughput enzyme engineering using a DmpR-dependent transcriptional activation system
por: Kwon, Kil Koang, et al.
Publicado: (2020)

A synthetic microbial biosensor for high-throughput screening of lactam biocatalysts
por: Yeom, Soo-Jin, et al.
Publicado: (2018)

Novel High-Throughput DNA Part Characterization Technique for Synthetic Biology
por: Bak, Seong-Kun, et al.
Publicado: (2022)

Molecular and biochemical characterization of a novel isoprene synthase from Metrosideros polymorpha
por: Yeom, Soo-Jin, et al.
Publicado: (2018)

Regioselective One-Pot Synthesis of Hydroxy-(S)-Equols Using Isoflavonoid Reductases and Monooxygenases and Evaluation of the Hydroxyequol Derivatives as Selective Estrogen Receptor Modulators and Antioxidants
por: Song, Hanbit, et al.
Publicado: (2022)

C1 Compound Biosensors: Design, Functional Study, and Applications
por: Lee, Jin-Young, et al.
Publicado: (2019)

Electron Transfer to Hydroxylase through Component Interactions in Soluble Methane Monooxygenase
por: Lee, Chaemin, et al.
Publicado: (2022)

Controlled Aggregation and Increased Stability of β-Glucuronidase by Cellulose Binding Domain Fusion
por: Yeom, Soo-Jin, et al.
Publicado: (2017)

MMOD-induced structural changes of hydroxylase in soluble methane monooxygenase
por: Kim, Hanseong, et al.
Publicado: (2019)

Methanol Dehydrogenases as a Key Biocatalysts for Synthetic Methylotrophy
por: Le, Thien-Kim, et al.
Publicado: (2021)

Translational Detection of Indole by Complementary Cell-free Protein Synthesis Assay
por: Lee, You Jin, et al.
Publicado: (2022)

Long-Term Stable and Tightly Controlled Expression of Recombinant Proteins in Antibiotics-Free Conditions
por: Yeom, Soo-Jin, et al.
Publicado: (2016)

Production of D-Allose From D-Allulose Using Commercial Immobilized Glucose Isomerase
por: Choi, Mi Na, et al.
Publicado: (2021)

Electron Transfer Control in Soluble Methane Monooxygenase
por: Wang, Weixue, et al.
Publicado: (2014)

CRISPR interference‐mediated gene regulation in Pseudomonas putida KT2440
por: Kim, Seong Keun, et al.
Publicado: (2019)

Deep learning with microfluidics for on-chip droplet generation, control, and analysis
por: Sun, Hao, et al.
Publicado: (2023)

A Portable Droplet Magnetofluidic Device for Point-of-Care Detection of Multidrug-Resistant Candida auris
por: Lee, Pei-Wei, et al.
Publicado: (2022)

A Genetically Encoded Biosensor for the Detection of Levulinic Acid
por: Kim, Tae Hyun, et al.
Publicado: (2023)

Maximizing Biocatalytic Cyclohexane Hydroxylation by Modulating Cytochrome P450 Monooxygenase Expression in P. taiwanensis VLB120
por: Schäfer, Lisa, et al.
Publicado: (2020)

Photosynthetic Constraints on Fuel from Microbes
por: Cotton, Charles A. R., et al.
Publicado: (2015)

Biofuels: From Microbes to Molecules (Book Review)
por: Akhtar, M. Kalim, et al.
Publicado: (2015)

Engineering Microbes to Bio-Upcycle Polyethylene Terephthalate
por: Dissanayake, Lakshika, et al.
Publicado: (2021)

Bioprospecting microbes and enzymes for the production of pterocarpans and coumestans
por: Perez Rojo, Fernando, et al.
Publicado: (2023)

Editorial: Environmental bioremediation: application of enzymes and microbes
por: Cheng, Xiyu, et al.
Publicado: (2023)

Production of Cinnamic and p-Hydroxycinnamic Acids in Engineered Microbes
por: Vargas-Tah, Alejandra, et al.
Publicado: (2015)

Photocatalytic Material–Microbe Hybrids: Applications in Environmental Remediations
por: Yu, Yadong, et al.
Publicado: (2022)

Evolution of enzymes with new specificity by high-throughput screening using DmpR-based genetic circuits and multiple flow cytometry rounds
por: Kwon, Kil Koang, et al.
Publicado: (2018)

Production of Recombinant Horseradish Peroxidase in an Engineered Cell-free Protein Synthesis System
por: Park, Yu-Jin, et al.
Publicado: (2021)

Complete Genome Sequence of the Soil Bacterium Pseudomonas kribbensis Strain 46-2(T)
por: Jeong, Haeyoung, et al.
Publicado: (2018)

Production of Fatty Acid-Derived Valuable Chemicals in Synthetic Microbes
por: Yu, Ai-Qun, et al.
Publicado: (2014)

Editorial: Pathway, Genetic and Process Engineering of Microbes for Biopolymer Synthesis
por: Poblete-Castro, Ignacio, et al.
Publicado: (2020)

Editorial: Synthetic biology for the sustainable production of biochemicals in engineered microbes
por: Shi, Shuobo, et al.
Publicado: (2022)

Harnessing synthetic biology for sustainable biomining with Fe/S-oxidizing microbes
por: Chen, Jinjin, et al.
Publicado: (2022)

Ammonium Impacts Methane Oxidation and Methanotrophic Community in Freshwater Sediment
por: Yang, Yuyin, et al.
Publicado: (2020)

An Integrative Multiomics Approach to Characterize Prebiotic Inulin Effects on Faecalibacterium prausnitzii
por: Park, Ji-Hyeon, et al.
Publicado: (2022)

Recent Progress and Advances in Stimuli-Responsive Polymers for Cancer Therapy
por: Rao, N. Vijayakameswara, et al.
Publicado: (2018)

Granular Carbon-Based Electrodes as Cathodes in Methane-Producing Bioelectrochemical Systems
por: Liu, Dandan, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_oo7ggi9c40j3gbavehhgeh4f65