Taking advantage of a high-throughput flow cytometer for the implementation of an ADCC assay for regulatory compliance

Technological advances allowed the development of high-throughput instruments such as IntelliCyt iQue Screener PLUS®. Here, we took advantage of this technology to transfer a previously validated cytotoxicity assay. The evaluated parameters were cell permeability, caspase activation and phosphatidyl...

Descripción completa

Detalles Bibliográficos
Autores principales: Camacho-Sandoval, Rosa, Jiménez-Uribe, Alexis, Tenorio-Calvo, Alejandra V., López-Morales, Carlos A., Muñoz-García, Leslie, Montes-Luna, Alejandra, García-Xolalpa, Héctor Leonardo, Velasco-Velázquez, Marco, Pavón, Lenin, Pérez-Tapia, Sonia Mayra, Medina-Rivero, Emilio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7193121/
https://www.ncbi.nlm.nih.gov/pubmed/32373484
http://dx.doi.org/10.1016/j.btre.2020.e00456
Descripción
Sumario:Technological advances allowed the development of high-throughput instruments such as IntelliCyt iQue Screener PLUS®. Here, we took advantage of this technology to transfer a previously validated cytotoxicity assay. The evaluated parameters were cell permeability, caspase activation and phosphatidyl serine exposure. The assay was accurate (r(2) = 0.90), precise (%CV ≤ 18.90) and specific. These results showed that this technology is suitable to be used in control quality environments. In addition, the automation provided a faster acquisition and analysis of data with precise and accurate results. This application could be implemented to evaluate another in vitro mechanism of action of different biotherapeutics.

Ejemplares similares