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Proteome Multimarker Panel With Multiple Reaction Monitoring–Mass Spectrometry for Early Detection of Hepatocellular Carcinoma

There is an urgent need for new biomarkers that address the shortcomings of current screening methods which fail to detect a large proportion of cases with hepatocellular carcinoma (HCC) at early stage. To develop a robust, multiple‐biomarker panel based on multiple reaction monitoring–mass spectrom...

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Autores principales: Yeo, Injoon, Kim, Gi‐Ae, Kim, Hyunsoo, Lee, Ji Hyeon, Sohn, Areum, Gwak, Geum‐Youn, Lee, Jeong‐Hoon, Lim, Young‐Suk, Kim, Youngsoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7193127/
https://www.ncbi.nlm.nih.gov/pubmed/32363324
http://dx.doi.org/10.1002/hep4.1500
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author Yeo, Injoon
Kim, Gi‐Ae
Kim, Hyunsoo
Lee, Ji Hyeon
Sohn, Areum
Gwak, Geum‐Youn
Lee, Jeong‐Hoon
Lim, Young‐Suk
Kim, Youngsoo
author_facet Yeo, Injoon
Kim, Gi‐Ae
Kim, Hyunsoo
Lee, Ji Hyeon
Sohn, Areum
Gwak, Geum‐Youn
Lee, Jeong‐Hoon
Lim, Young‐Suk
Kim, Youngsoo
author_sort Yeo, Injoon
collection PubMed
description There is an urgent need for new biomarkers that address the shortcomings of current screening methods which fail to detect a large proportion of cases with hepatocellular carcinoma (HCC) at early stage. To develop a robust, multiple‐biomarker panel based on multiple reaction monitoring–mass spectrometry with high performance in detecting early‐stage HCC within at‐risk populations. In the discovery set, 150 samples were analyzed to identify candidate biomarkers. The resulting list of candidates was tested in the training set (713 samples) to establish a multimarker panel, which was evaluated in the validation set (305 samples). We identified 385 serum HCC biomarker candidates in the discovery set and developed a multimarker panel consisting of 28 peptides that best differentiated HCC from controls. The area under the receiver operating characteristic curve of multimarker panel was significantly higher than alpha‐fetoprotein (AFP) in the training (0.976 vs. 0.804; P < 0.001) and validation (0.898 vs. 0.778; P < 0.001) sets. In the validation set, this multimarker panel, compared with AFP, showed significantly greater sensitivity (81.1% vs. 26.8%; P < 0.001) and lower specificity (84.8% vs. 98.8%; P < 0.001) in detecting HCC cases. Combining AFP with the multimarker panel did not significantly improve the area under the receiver operating characteristic curve compared with the panel alone in the training (0.981 vs. 0.976; P = 0.37) and validation set (0.906 vs. 0.898; P = 0.75). Conclusion: The multiple reaction monitoring–mass spectrometry multimarker panel consisting of 28 peptides discriminates HCC cases from at‐risk controls with high performance and may have potential for clinical application in HCC surveillance.
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spelling pubmed-71931272020-05-01 Proteome Multimarker Panel With Multiple Reaction Monitoring–Mass Spectrometry for Early Detection of Hepatocellular Carcinoma Yeo, Injoon Kim, Gi‐Ae Kim, Hyunsoo Lee, Ji Hyeon Sohn, Areum Gwak, Geum‐Youn Lee, Jeong‐Hoon Lim, Young‐Suk Kim, Youngsoo Hepatol Commun Original Articles There is an urgent need for new biomarkers that address the shortcomings of current screening methods which fail to detect a large proportion of cases with hepatocellular carcinoma (HCC) at early stage. To develop a robust, multiple‐biomarker panel based on multiple reaction monitoring–mass spectrometry with high performance in detecting early‐stage HCC within at‐risk populations. In the discovery set, 150 samples were analyzed to identify candidate biomarkers. The resulting list of candidates was tested in the training set (713 samples) to establish a multimarker panel, which was evaluated in the validation set (305 samples). We identified 385 serum HCC biomarker candidates in the discovery set and developed a multimarker panel consisting of 28 peptides that best differentiated HCC from controls. The area under the receiver operating characteristic curve of multimarker panel was significantly higher than alpha‐fetoprotein (AFP) in the training (0.976 vs. 0.804; P < 0.001) and validation (0.898 vs. 0.778; P < 0.001) sets. In the validation set, this multimarker panel, compared with AFP, showed significantly greater sensitivity (81.1% vs. 26.8%; P < 0.001) and lower specificity (84.8% vs. 98.8%; P < 0.001) in detecting HCC cases. Combining AFP with the multimarker panel did not significantly improve the area under the receiver operating characteristic curve compared with the panel alone in the training (0.981 vs. 0.976; P = 0.37) and validation set (0.906 vs. 0.898; P = 0.75). Conclusion: The multiple reaction monitoring–mass spectrometry multimarker panel consisting of 28 peptides discriminates HCC cases from at‐risk controls with high performance and may have potential for clinical application in HCC surveillance. John Wiley and Sons Inc. 2020-03-13 /pmc/articles/PMC7193127/ /pubmed/32363324 http://dx.doi.org/10.1002/hep4.1500 Text en © 2020 The Authors. Hepatology Communications published by Wiley Periodicals, Inc., on behalf of the American Association for the Study of Liver Diseases. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Yeo, Injoon
Kim, Gi‐Ae
Kim, Hyunsoo
Lee, Ji Hyeon
Sohn, Areum
Gwak, Geum‐Youn
Lee, Jeong‐Hoon
Lim, Young‐Suk
Kim, Youngsoo
Proteome Multimarker Panel With Multiple Reaction Monitoring–Mass Spectrometry for Early Detection of Hepatocellular Carcinoma
title Proteome Multimarker Panel With Multiple Reaction Monitoring–Mass Spectrometry for Early Detection of Hepatocellular Carcinoma
title_full Proteome Multimarker Panel With Multiple Reaction Monitoring–Mass Spectrometry for Early Detection of Hepatocellular Carcinoma
title_fullStr Proteome Multimarker Panel With Multiple Reaction Monitoring–Mass Spectrometry for Early Detection of Hepatocellular Carcinoma
title_full_unstemmed Proteome Multimarker Panel With Multiple Reaction Monitoring–Mass Spectrometry for Early Detection of Hepatocellular Carcinoma
title_short Proteome Multimarker Panel With Multiple Reaction Monitoring–Mass Spectrometry for Early Detection of Hepatocellular Carcinoma
title_sort proteome multimarker panel with multiple reaction monitoring–mass spectrometry for early detection of hepatocellular carcinoma
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7193127/
https://www.ncbi.nlm.nih.gov/pubmed/32363324
http://dx.doi.org/10.1002/hep4.1500
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