Cargando…

Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida)

In the present study, we profiled bacterial and archaeal communities from 13 phylogenetically diverse deep-sea sponge species (Demospongiae and Hexactinellida) from the South Pacific by 16S rRNA-gene amplicon sequencing. Additionally, the associated bacteria and archaea were quantified by real-time...

Descripción completa

Detalles Bibliográficos
Autores principales: Steinert, Georg, Busch, Kathrin, Bayer, Kristina, Kodami, Sahar, Arbizu, Pedro Martinez, Kelly, Michelle, Mills, Sadie, Erpenbeck, Dirk, Dohrmann, Martin, Wörheide, Gert, Hentschel, Ute, Schupp, Peter J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7193145/
https://www.ncbi.nlm.nih.gov/pubmed/32390977
http://dx.doi.org/10.3389/fmicb.2020.00716
_version_ 1783528137042165760
author Steinert, Georg
Busch, Kathrin
Bayer, Kristina
Kodami, Sahar
Arbizu, Pedro Martinez
Kelly, Michelle
Mills, Sadie
Erpenbeck, Dirk
Dohrmann, Martin
Wörheide, Gert
Hentschel, Ute
Schupp, Peter J.
author_facet Steinert, Georg
Busch, Kathrin
Bayer, Kristina
Kodami, Sahar
Arbizu, Pedro Martinez
Kelly, Michelle
Mills, Sadie
Erpenbeck, Dirk
Dohrmann, Martin
Wörheide, Gert
Hentschel, Ute
Schupp, Peter J.
author_sort Steinert, Georg
collection PubMed
description In the present study, we profiled bacterial and archaeal communities from 13 phylogenetically diverse deep-sea sponge species (Demospongiae and Hexactinellida) from the South Pacific by 16S rRNA-gene amplicon sequencing. Additionally, the associated bacteria and archaea were quantified by real-time qPCR. Our results show that bacterial communities from the deep-sea sponges are mostly host-species specific similar to what has been observed for shallow-water demosponges. The archaeal deep-sea sponge community structures are different from the bacterial community structures in that they are almost completely dominated by a single family, which are the ammonia-oxidizing genera within the Nitrosopumilaceae. Remarkably, the archaeal communities are mostly specific to individual sponges (rather than sponge-species), and this observation applies to both hexactinellids and demosponges. Finally, archaeal 16s gene numbers, as detected by quantitative real-time PCR, were up to three orders of magnitude higher than in shallow-water sponges, highlighting the importance of the archaea for deep-sea sponges in general.
format Online
Article
Text
id pubmed-7193145
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-71931452020-05-08 Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida) Steinert, Georg Busch, Kathrin Bayer, Kristina Kodami, Sahar Arbizu, Pedro Martinez Kelly, Michelle Mills, Sadie Erpenbeck, Dirk Dohrmann, Martin Wörheide, Gert Hentschel, Ute Schupp, Peter J. Front Microbiol Microbiology In the present study, we profiled bacterial and archaeal communities from 13 phylogenetically diverse deep-sea sponge species (Demospongiae and Hexactinellida) from the South Pacific by 16S rRNA-gene amplicon sequencing. Additionally, the associated bacteria and archaea were quantified by real-time qPCR. Our results show that bacterial communities from the deep-sea sponges are mostly host-species specific similar to what has been observed for shallow-water demosponges. The archaeal deep-sea sponge community structures are different from the bacterial community structures in that they are almost completely dominated by a single family, which are the ammonia-oxidizing genera within the Nitrosopumilaceae. Remarkably, the archaeal communities are mostly specific to individual sponges (rather than sponge-species), and this observation applies to both hexactinellids and demosponges. Finally, archaeal 16s gene numbers, as detected by quantitative real-time PCR, were up to three orders of magnitude higher than in shallow-water sponges, highlighting the importance of the archaea for deep-sea sponges in general. Frontiers Media S.A. 2020-04-24 /pmc/articles/PMC7193145/ /pubmed/32390977 http://dx.doi.org/10.3389/fmicb.2020.00716 Text en Copyright © 2020 Steinert, Busch, Bayer, Kodami, Arbizu, Kelly, Mills, Erpenbeck, Dohrmann, Wörheide, Hentschel and Schupp. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Steinert, Georg
Busch, Kathrin
Bayer, Kristina
Kodami, Sahar
Arbizu, Pedro Martinez
Kelly, Michelle
Mills, Sadie
Erpenbeck, Dirk
Dohrmann, Martin
Wörheide, Gert
Hentschel, Ute
Schupp, Peter J.
Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida)
title Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida)
title_full Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida)
title_fullStr Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida)
title_full_unstemmed Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida)
title_short Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida)
title_sort compositional and quantitative insights into bacterial and archaeal communities of south pacific deep-sea sponges (demospongiae and hexactinellida)
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7193145/
https://www.ncbi.nlm.nih.gov/pubmed/32390977
http://dx.doi.org/10.3389/fmicb.2020.00716
work_keys_str_mv AT steinertgeorg compositionalandquantitativeinsightsintobacterialandarchaealcommunitiesofsouthpacificdeepseaspongesdemospongiaeandhexactinellida
AT buschkathrin compositionalandquantitativeinsightsintobacterialandarchaealcommunitiesofsouthpacificdeepseaspongesdemospongiaeandhexactinellida
AT bayerkristina compositionalandquantitativeinsightsintobacterialandarchaealcommunitiesofsouthpacificdeepseaspongesdemospongiaeandhexactinellida
AT kodamisahar compositionalandquantitativeinsightsintobacterialandarchaealcommunitiesofsouthpacificdeepseaspongesdemospongiaeandhexactinellida
AT arbizupedromartinez compositionalandquantitativeinsightsintobacterialandarchaealcommunitiesofsouthpacificdeepseaspongesdemospongiaeandhexactinellida
AT kellymichelle compositionalandquantitativeinsightsintobacterialandarchaealcommunitiesofsouthpacificdeepseaspongesdemospongiaeandhexactinellida
AT millssadie compositionalandquantitativeinsightsintobacterialandarchaealcommunitiesofsouthpacificdeepseaspongesdemospongiaeandhexactinellida
AT erpenbeckdirk compositionalandquantitativeinsightsintobacterialandarchaealcommunitiesofsouthpacificdeepseaspongesdemospongiaeandhexactinellida
AT dohrmannmartin compositionalandquantitativeinsightsintobacterialandarchaealcommunitiesofsouthpacificdeepseaspongesdemospongiaeandhexactinellida
AT worheidegert compositionalandquantitativeinsightsintobacterialandarchaealcommunitiesofsouthpacificdeepseaspongesdemospongiaeandhexactinellida
AT hentschelute compositionalandquantitativeinsightsintobacterialandarchaealcommunitiesofsouthpacificdeepseaspongesdemospongiaeandhexactinellida
AT schupppeterj compositionalandquantitativeinsightsintobacterialandarchaealcommunitiesofsouthpacificdeepseaspongesdemospongiaeandhexactinellida