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Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida)
In the present study, we profiled bacterial and archaeal communities from 13 phylogenetically diverse deep-sea sponge species (Demospongiae and Hexactinellida) from the South Pacific by 16S rRNA-gene amplicon sequencing. Additionally, the associated bacteria and archaea were quantified by real-time...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7193145/ https://www.ncbi.nlm.nih.gov/pubmed/32390977 http://dx.doi.org/10.3389/fmicb.2020.00716 |
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author | Steinert, Georg Busch, Kathrin Bayer, Kristina Kodami, Sahar Arbizu, Pedro Martinez Kelly, Michelle Mills, Sadie Erpenbeck, Dirk Dohrmann, Martin Wörheide, Gert Hentschel, Ute Schupp, Peter J. |
author_facet | Steinert, Georg Busch, Kathrin Bayer, Kristina Kodami, Sahar Arbizu, Pedro Martinez Kelly, Michelle Mills, Sadie Erpenbeck, Dirk Dohrmann, Martin Wörheide, Gert Hentschel, Ute Schupp, Peter J. |
author_sort | Steinert, Georg |
collection | PubMed |
description | In the present study, we profiled bacterial and archaeal communities from 13 phylogenetically diverse deep-sea sponge species (Demospongiae and Hexactinellida) from the South Pacific by 16S rRNA-gene amplicon sequencing. Additionally, the associated bacteria and archaea were quantified by real-time qPCR. Our results show that bacterial communities from the deep-sea sponges are mostly host-species specific similar to what has been observed for shallow-water demosponges. The archaeal deep-sea sponge community structures are different from the bacterial community structures in that they are almost completely dominated by a single family, which are the ammonia-oxidizing genera within the Nitrosopumilaceae. Remarkably, the archaeal communities are mostly specific to individual sponges (rather than sponge-species), and this observation applies to both hexactinellids and demosponges. Finally, archaeal 16s gene numbers, as detected by quantitative real-time PCR, were up to three orders of magnitude higher than in shallow-water sponges, highlighting the importance of the archaea for deep-sea sponges in general. |
format | Online Article Text |
id | pubmed-7193145 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71931452020-05-08 Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida) Steinert, Georg Busch, Kathrin Bayer, Kristina Kodami, Sahar Arbizu, Pedro Martinez Kelly, Michelle Mills, Sadie Erpenbeck, Dirk Dohrmann, Martin Wörheide, Gert Hentschel, Ute Schupp, Peter J. Front Microbiol Microbiology In the present study, we profiled bacterial and archaeal communities from 13 phylogenetically diverse deep-sea sponge species (Demospongiae and Hexactinellida) from the South Pacific by 16S rRNA-gene amplicon sequencing. Additionally, the associated bacteria and archaea were quantified by real-time qPCR. Our results show that bacterial communities from the deep-sea sponges are mostly host-species specific similar to what has been observed for shallow-water demosponges. The archaeal deep-sea sponge community structures are different from the bacterial community structures in that they are almost completely dominated by a single family, which are the ammonia-oxidizing genera within the Nitrosopumilaceae. Remarkably, the archaeal communities are mostly specific to individual sponges (rather than sponge-species), and this observation applies to both hexactinellids and demosponges. Finally, archaeal 16s gene numbers, as detected by quantitative real-time PCR, were up to three orders of magnitude higher than in shallow-water sponges, highlighting the importance of the archaea for deep-sea sponges in general. Frontiers Media S.A. 2020-04-24 /pmc/articles/PMC7193145/ /pubmed/32390977 http://dx.doi.org/10.3389/fmicb.2020.00716 Text en Copyright © 2020 Steinert, Busch, Bayer, Kodami, Arbizu, Kelly, Mills, Erpenbeck, Dohrmann, Wörheide, Hentschel and Schupp. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Steinert, Georg Busch, Kathrin Bayer, Kristina Kodami, Sahar Arbizu, Pedro Martinez Kelly, Michelle Mills, Sadie Erpenbeck, Dirk Dohrmann, Martin Wörheide, Gert Hentschel, Ute Schupp, Peter J. Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida) |
title | Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida) |
title_full | Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida) |
title_fullStr | Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida) |
title_full_unstemmed | Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida) |
title_short | Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida) |
title_sort | compositional and quantitative insights into bacterial and archaeal communities of south pacific deep-sea sponges (demospongiae and hexactinellida) |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7193145/ https://www.ncbi.nlm.nih.gov/pubmed/32390977 http://dx.doi.org/10.3389/fmicb.2020.00716 |
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