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Multiaxial Lenticular Stress-Strain Relationship of Native Myocardium is Preserved by Infarct-Induced Natural Heart Regeneration in Neonatal Mice
Neonatal mice exhibit natural heart regeneration after myocardial infarction (MI) on postnatal day 1 (P1), but this ability is lost by postnatal day 7 (P7). Cardiac biomechanics intricately affect long-term heart function, but whether regenerated cardiac muscle is biomechanically similar to native m...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7193551/ https://www.ncbi.nlm.nih.gov/pubmed/32355240 http://dx.doi.org/10.1038/s41598-020-63324-w |
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author | Wang, Hanjay Bennett-Kennett, Ross Paulsen, Michael J. Hironaka, Camille E. Thakore, Akshara D. Farry, Justin M. Eskandari, Anahita Lucian, Haley J. Shin, Hye Sook Wu, Matthew A. Imbrie-Moore, Annabel M. Steele, Amanda N. Stapleton, Lyndsay M. Zhu, Yuanjia Dauskardt, Reinhold H. Woo, Y. Joseph |
author_facet | Wang, Hanjay Bennett-Kennett, Ross Paulsen, Michael J. Hironaka, Camille E. Thakore, Akshara D. Farry, Justin M. Eskandari, Anahita Lucian, Haley J. Shin, Hye Sook Wu, Matthew A. Imbrie-Moore, Annabel M. Steele, Amanda N. Stapleton, Lyndsay M. Zhu, Yuanjia Dauskardt, Reinhold H. Woo, Y. Joseph |
author_sort | Wang, Hanjay |
collection | PubMed |
description | Neonatal mice exhibit natural heart regeneration after myocardial infarction (MI) on postnatal day 1 (P1), but this ability is lost by postnatal day 7 (P7). Cardiac biomechanics intricately affect long-term heart function, but whether regenerated cardiac muscle is biomechanically similar to native myocardium remains unknown. We hypothesized that neonatal heart regeneration preserves native left ventricular (LV) biomechanical properties after MI. C57BL/6J mice underwent sham surgery or left anterior descending coronary artery ligation at age P1 or P7. Echocardiography performed 4 weeks post-MI showed that P1 MI and sham mice (n = 22, each) had similar LV wall thickness, diameter, and ejection fraction (59.6% vs 60.7%, p = 0.6514). Compared to P7 shams (n = 20), P7 MI mice (n = 20) had significant LV wall thinning, chamber enlargement, and depressed ejection fraction (32.6% vs 61.8%, p < 0.0001). Afterward, the LV was explanted and pressurized ex vivo, and the multiaxial lenticular stress-strain relationship was tracked. While LV tissue modulus for P1 MI and sham mice were similar (341.9 kPa vs 363.4 kPa, p = 0.6140), the modulus for P7 MI mice was significantly greater than that for P7 shams (691.6 kPa vs 429.2 kPa, p = 0.0194). We conclude that, in neonatal mice, regenerated LV muscle has similar biomechanical properties as native LV myocardium. |
format | Online Article Text |
id | pubmed-7193551 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-71935512020-05-08 Multiaxial Lenticular Stress-Strain Relationship of Native Myocardium is Preserved by Infarct-Induced Natural Heart Regeneration in Neonatal Mice Wang, Hanjay Bennett-Kennett, Ross Paulsen, Michael J. Hironaka, Camille E. Thakore, Akshara D. Farry, Justin M. Eskandari, Anahita Lucian, Haley J. Shin, Hye Sook Wu, Matthew A. Imbrie-Moore, Annabel M. Steele, Amanda N. Stapleton, Lyndsay M. Zhu, Yuanjia Dauskardt, Reinhold H. Woo, Y. Joseph Sci Rep Article Neonatal mice exhibit natural heart regeneration after myocardial infarction (MI) on postnatal day 1 (P1), but this ability is lost by postnatal day 7 (P7). Cardiac biomechanics intricately affect long-term heart function, but whether regenerated cardiac muscle is biomechanically similar to native myocardium remains unknown. We hypothesized that neonatal heart regeneration preserves native left ventricular (LV) biomechanical properties after MI. C57BL/6J mice underwent sham surgery or left anterior descending coronary artery ligation at age P1 or P7. Echocardiography performed 4 weeks post-MI showed that P1 MI and sham mice (n = 22, each) had similar LV wall thickness, diameter, and ejection fraction (59.6% vs 60.7%, p = 0.6514). Compared to P7 shams (n = 20), P7 MI mice (n = 20) had significant LV wall thinning, chamber enlargement, and depressed ejection fraction (32.6% vs 61.8%, p < 0.0001). Afterward, the LV was explanted and pressurized ex vivo, and the multiaxial lenticular stress-strain relationship was tracked. While LV tissue modulus for P1 MI and sham mice were similar (341.9 kPa vs 363.4 kPa, p = 0.6140), the modulus for P7 MI mice was significantly greater than that for P7 shams (691.6 kPa vs 429.2 kPa, p = 0.0194). We conclude that, in neonatal mice, regenerated LV muscle has similar biomechanical properties as native LV myocardium. Nature Publishing Group UK 2020-04-30 /pmc/articles/PMC7193551/ /pubmed/32355240 http://dx.doi.org/10.1038/s41598-020-63324-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Wang, Hanjay Bennett-Kennett, Ross Paulsen, Michael J. Hironaka, Camille E. Thakore, Akshara D. Farry, Justin M. Eskandari, Anahita Lucian, Haley J. Shin, Hye Sook Wu, Matthew A. Imbrie-Moore, Annabel M. Steele, Amanda N. Stapleton, Lyndsay M. Zhu, Yuanjia Dauskardt, Reinhold H. Woo, Y. Joseph Multiaxial Lenticular Stress-Strain Relationship of Native Myocardium is Preserved by Infarct-Induced Natural Heart Regeneration in Neonatal Mice |
title | Multiaxial Lenticular Stress-Strain Relationship of Native Myocardium is Preserved by Infarct-Induced Natural Heart Regeneration in Neonatal Mice |
title_full | Multiaxial Lenticular Stress-Strain Relationship of Native Myocardium is Preserved by Infarct-Induced Natural Heart Regeneration in Neonatal Mice |
title_fullStr | Multiaxial Lenticular Stress-Strain Relationship of Native Myocardium is Preserved by Infarct-Induced Natural Heart Regeneration in Neonatal Mice |
title_full_unstemmed | Multiaxial Lenticular Stress-Strain Relationship of Native Myocardium is Preserved by Infarct-Induced Natural Heart Regeneration in Neonatal Mice |
title_short | Multiaxial Lenticular Stress-Strain Relationship of Native Myocardium is Preserved by Infarct-Induced Natural Heart Regeneration in Neonatal Mice |
title_sort | multiaxial lenticular stress-strain relationship of native myocardium is preserved by infarct-induced natural heart regeneration in neonatal mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7193551/ https://www.ncbi.nlm.nih.gov/pubmed/32355240 http://dx.doi.org/10.1038/s41598-020-63324-w |
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