Integrated Human Immunodeficiency Virus Type 1 Sequence in J-Lat 10.6

The full length of HIV/R7/E(−)/GFP integrated in the J-Lat 10.6 cell line was sequenced in this study. The single copy of the integrated virus, including the breakpoints from the human chromosome to the provirus, was amplified by two separate PCRs. A 10,200-bp genome sequence was acquired, analyzed,...

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Autores principales: Chung, Cheng-Han, Mele, Anthony R., Allen, Alexander G., Costello, Robert, Dampier, Will, Nonnemacher, Michael R., Wigdahl, Brian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2020
Materias:
Genome Sequences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7193928/
https://www.ncbi.nlm.nih.gov/pubmed/32354973
http://dx.doi.org/10.1128/MRA.00179-20
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author Chung, Cheng-Han
Mele, Anthony R.
Allen, Alexander G.
Costello, Robert
Dampier, Will
Nonnemacher, Michael R.
Wigdahl, Brian
author_facet Chung, Cheng-Han
Mele, Anthony R.
Allen, Alexander G.
Costello, Robert
Dampier, Will
Nonnemacher, Michael R.
Wigdahl, Brian
author_sort Chung, Cheng-Han
collection PubMed
description The full length of HIV/R7/E(−)/GFP integrated in the J-Lat 10.6 cell line was sequenced in this study. The single copy of the integrated virus, including the breakpoints from the human chromosome to the provirus, was amplified by two separate PCRs. A 10,200-bp genome sequence was acquired, analyzed, and deposited in GenBank.
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spelling pubmed-71939282020-05-07 Integrated Human Immunodeficiency Virus Type 1 Sequence in J-Lat 10.6 Chung, Cheng-Han Mele, Anthony R. Allen, Alexander G. Costello, Robert Dampier, Will Nonnemacher, Michael R. Wigdahl, Brian Microbiol Resour Announc Genome Sequences The full length of HIV/R7/E(−)/GFP integrated in the J-Lat 10.6 cell line was sequenced in this study. The single copy of the integrated virus, including the breakpoints from the human chromosome to the provirus, was amplified by two separate PCRs. A 10,200-bp genome sequence was acquired, analyzed, and deposited in GenBank. American Society for Microbiology 2020-04-30 /pmc/articles/PMC7193928/ /pubmed/32354973 http://dx.doi.org/10.1128/MRA.00179-20 Text en Copyright © 2020 Chung et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Genome Sequences
Chung, Cheng-Han
Mele, Anthony R.
Allen, Alexander G.
Costello, Robert
Dampier, Will
Nonnemacher, Michael R.
Wigdahl, Brian
Integrated Human Immunodeficiency Virus Type 1 Sequence in J-Lat 10.6
title Integrated Human Immunodeficiency Virus Type 1 Sequence in J-Lat 10.6
title_full Integrated Human Immunodeficiency Virus Type 1 Sequence in J-Lat 10.6
title_fullStr Integrated Human Immunodeficiency Virus Type 1 Sequence in J-Lat 10.6
title_full_unstemmed Integrated Human Immunodeficiency Virus Type 1 Sequence in J-Lat 10.6
title_short Integrated Human Immunodeficiency Virus Type 1 Sequence in J-Lat 10.6
title_sort integrated human immunodeficiency virus type 1 sequence in j-lat 10.6
topic Genome Sequences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7193928/
https://www.ncbi.nlm.nih.gov/pubmed/32354973
http://dx.doi.org/10.1128/MRA.00179-20
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