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Assessment of the microbial interplay during anaerobic co-digestion of wastewater sludge using common components analysis

Anaerobic digestion (AD) is used to minimize solid waste while producing biogas by the action of microorganisms. To give an insight into the underlying microbial dynamics in anaerobic digesters, we investigated two different AD systems (wastewater sludge mixed with either fish or grass waste). The m...

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Autores principales: Puig-Castellví, Francesc, Cardona, Laëtitia, Jouan-Rimbaud Bouveresse, Delphine, Cordella, Christophe B. Y., Mazéas, Laurent, Rutledge, Douglas N., Chapleur, Olivier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7194399/
https://www.ncbi.nlm.nih.gov/pubmed/32357180
http://dx.doi.org/10.1371/journal.pone.0232324
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author Puig-Castellví, Francesc
Cardona, Laëtitia
Jouan-Rimbaud Bouveresse, Delphine
Cordella, Christophe B. Y.
Mazéas, Laurent
Rutledge, Douglas N.
Chapleur, Olivier
author_facet Puig-Castellví, Francesc
Cardona, Laëtitia
Jouan-Rimbaud Bouveresse, Delphine
Cordella, Christophe B. Y.
Mazéas, Laurent
Rutledge, Douglas N.
Chapleur, Olivier
author_sort Puig-Castellví, Francesc
collection PubMed
description Anaerobic digestion (AD) is used to minimize solid waste while producing biogas by the action of microorganisms. To give an insight into the underlying microbial dynamics in anaerobic digesters, we investigated two different AD systems (wastewater sludge mixed with either fish or grass waste). The microbial activity was characterized by 16S RNA sequencing. 16S data is sparse and dispersed, and existent data analysis methods do not take into account this complexity nor the potential microbial interactions. In this line, we proposed a data pre-processing pipeline addressing these issues while not restricting only to the most abundant microorganisms. The data were analyzed by Common Components Analysis (CCA) to decipher the effect of substrate composition on the microorganisms. CCA results hinted the relationships between the microorganisms responding similarly to the AD physicochemical parameters. Thus, in overall, CCA allowed a better understanding of the inter-species interactions within microbial communities.
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spelling pubmed-71943992020-05-12 Assessment of the microbial interplay during anaerobic co-digestion of wastewater sludge using common components analysis Puig-Castellví, Francesc Cardona, Laëtitia Jouan-Rimbaud Bouveresse, Delphine Cordella, Christophe B. Y. Mazéas, Laurent Rutledge, Douglas N. Chapleur, Olivier PLoS One Research Article Anaerobic digestion (AD) is used to minimize solid waste while producing biogas by the action of microorganisms. To give an insight into the underlying microbial dynamics in anaerobic digesters, we investigated two different AD systems (wastewater sludge mixed with either fish or grass waste). The microbial activity was characterized by 16S RNA sequencing. 16S data is sparse and dispersed, and existent data analysis methods do not take into account this complexity nor the potential microbial interactions. In this line, we proposed a data pre-processing pipeline addressing these issues while not restricting only to the most abundant microorganisms. The data were analyzed by Common Components Analysis (CCA) to decipher the effect of substrate composition on the microorganisms. CCA results hinted the relationships between the microorganisms responding similarly to the AD physicochemical parameters. Thus, in overall, CCA allowed a better understanding of the inter-species interactions within microbial communities. Public Library of Science 2020-05-01 /pmc/articles/PMC7194399/ /pubmed/32357180 http://dx.doi.org/10.1371/journal.pone.0232324 Text en © 2020 Puig-Castellví et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Puig-Castellví, Francesc
Cardona, Laëtitia
Jouan-Rimbaud Bouveresse, Delphine
Cordella, Christophe B. Y.
Mazéas, Laurent
Rutledge, Douglas N.
Chapleur, Olivier
Assessment of the microbial interplay during anaerobic co-digestion of wastewater sludge using common components analysis
title Assessment of the microbial interplay during anaerobic co-digestion of wastewater sludge using common components analysis
title_full Assessment of the microbial interplay during anaerobic co-digestion of wastewater sludge using common components analysis
title_fullStr Assessment of the microbial interplay during anaerobic co-digestion of wastewater sludge using common components analysis
title_full_unstemmed Assessment of the microbial interplay during anaerobic co-digestion of wastewater sludge using common components analysis
title_short Assessment of the microbial interplay during anaerobic co-digestion of wastewater sludge using common components analysis
title_sort assessment of the microbial interplay during anaerobic co-digestion of wastewater sludge using common components analysis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7194399/
https://www.ncbi.nlm.nih.gov/pubmed/32357180
http://dx.doi.org/10.1371/journal.pone.0232324
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