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Functional cycle of EEA1-positive early endosome: Direct evidence for pre-existing compartment of degradative pathway
Early endosomes, regarded as the main sorting station on endocytic pathway, are characterized by high frequency of homotypic fusions mediated by tethering protein EEA1. Despite intensive investigations, biogenesis of endosomes, boundaries between early and late endosomes, and process of cargo transi...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7194439/ https://www.ncbi.nlm.nih.gov/pubmed/32357161 http://dx.doi.org/10.1371/journal.pone.0232532 |
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author | Kamentseva, Rimma Kosheverova, Vera Kharchenko, Marianna Zlobina, Maria Salova, Anna Belyaeva, Tatiana Nikolsky, Nikolay Kornilova, Elena |
author_facet | Kamentseva, Rimma Kosheverova, Vera Kharchenko, Marianna Zlobina, Maria Salova, Anna Belyaeva, Tatiana Nikolsky, Nikolay Kornilova, Elena |
author_sort | Kamentseva, Rimma |
collection | PubMed |
description | Early endosomes, regarded as the main sorting station on endocytic pathway, are characterized by high frequency of homotypic fusions mediated by tethering protein EEA1. Despite intensive investigations, biogenesis of endosomes, boundaries between early and late endosomes, and process of cargo transition though them remain obscure. Here, using EGF/EGFR endocytosis as a model and confocal microscopy of fixed and live cells, we provide evidence favoring EEA1-vesicles being pre-existed vesicular compartment, that maintains its resident proteins’ level and is sensitive to biosynthetic, but not endocytic pathway disturbance. EEA1-vesicles directly fuse with incoming EGF/EGFR-vesicles into hybrid endosomes with separated EEA1- and EGFR-domains, thus providing a platform for rapid achievement of an excess of surface-derived membrane that is used to form intraluminal vesicles (ILVs). Thus, multivesicular structures colocalized with EEA1 are still early endosomes. “EEA1-cycle” ends by exclusion of EGFR-containing domains with ILVs inside that turns into MVE and restoration of initial EEA1-vesicles population. |
format | Online Article Text |
id | pubmed-7194439 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-71944392020-05-12 Functional cycle of EEA1-positive early endosome: Direct evidence for pre-existing compartment of degradative pathway Kamentseva, Rimma Kosheverova, Vera Kharchenko, Marianna Zlobina, Maria Salova, Anna Belyaeva, Tatiana Nikolsky, Nikolay Kornilova, Elena PLoS One Research Article Early endosomes, regarded as the main sorting station on endocytic pathway, are characterized by high frequency of homotypic fusions mediated by tethering protein EEA1. Despite intensive investigations, biogenesis of endosomes, boundaries between early and late endosomes, and process of cargo transition though them remain obscure. Here, using EGF/EGFR endocytosis as a model and confocal microscopy of fixed and live cells, we provide evidence favoring EEA1-vesicles being pre-existed vesicular compartment, that maintains its resident proteins’ level and is sensitive to biosynthetic, but not endocytic pathway disturbance. EEA1-vesicles directly fuse with incoming EGF/EGFR-vesicles into hybrid endosomes with separated EEA1- and EGFR-domains, thus providing a platform for rapid achievement of an excess of surface-derived membrane that is used to form intraluminal vesicles (ILVs). Thus, multivesicular structures colocalized with EEA1 are still early endosomes. “EEA1-cycle” ends by exclusion of EGFR-containing domains with ILVs inside that turns into MVE and restoration of initial EEA1-vesicles population. Public Library of Science 2020-05-01 /pmc/articles/PMC7194439/ /pubmed/32357161 http://dx.doi.org/10.1371/journal.pone.0232532 Text en © 2020 Kamentseva et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Kamentseva, Rimma Kosheverova, Vera Kharchenko, Marianna Zlobina, Maria Salova, Anna Belyaeva, Tatiana Nikolsky, Nikolay Kornilova, Elena Functional cycle of EEA1-positive early endosome: Direct evidence for pre-existing compartment of degradative pathway |
title | Functional cycle of EEA1-positive early endosome: Direct evidence for pre-existing compartment of degradative pathway |
title_full | Functional cycle of EEA1-positive early endosome: Direct evidence for pre-existing compartment of degradative pathway |
title_fullStr | Functional cycle of EEA1-positive early endosome: Direct evidence for pre-existing compartment of degradative pathway |
title_full_unstemmed | Functional cycle of EEA1-positive early endosome: Direct evidence for pre-existing compartment of degradative pathway |
title_short | Functional cycle of EEA1-positive early endosome: Direct evidence for pre-existing compartment of degradative pathway |
title_sort | functional cycle of eea1-positive early endosome: direct evidence for pre-existing compartment of degradative pathway |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7194439/ https://www.ncbi.nlm.nih.gov/pubmed/32357161 http://dx.doi.org/10.1371/journal.pone.0232532 |
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