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Discovery of a new species of the Hypoxylon rubiginosum complex from Iran and antagonistic activities of Hypoxylon spp. against the Ash Dieback pathogen, Hymenoscyphus fraxineus, in dual culture

During a survey of xylarialean fungi in Northern Iran, several specimens that showed affinities to the Hypoxylon rubiginosum complex were collected and cultured. A comparison of their morphological characters, combined with a chemotaxonomic study based on high performance liquid chromatography, coup...

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Autores principales: Pourmoghaddam, Mohammad Javad, Lambert, Christopher, Surup, Frank, Khodaparast, Seyed Akbar, Krisai-Greilhuber, Irmgard, Voglmayr, Hermann, Stadler, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7195382/
https://www.ncbi.nlm.nih.gov/pubmed/32377154
http://dx.doi.org/10.3897/mycokeys.66.50946
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author Pourmoghaddam, Mohammad Javad
Lambert, Christopher
Surup, Frank
Khodaparast, Seyed Akbar
Krisai-Greilhuber, Irmgard
Voglmayr, Hermann
Stadler, Marc
author_facet Pourmoghaddam, Mohammad Javad
Lambert, Christopher
Surup, Frank
Khodaparast, Seyed Akbar
Krisai-Greilhuber, Irmgard
Voglmayr, Hermann
Stadler, Marc
author_sort Pourmoghaddam, Mohammad Javad
collection PubMed
description During a survey of xylarialean fungi in Northern Iran, several specimens that showed affinities to the Hypoxylon rubiginosum complex were collected and cultured. A comparison of their morphological characters, combined with a chemotaxonomic study based on high performance liquid chromatography, coupled with diode array detection and mass spectrometry (HPLC-DAD/MS) and a multi-locus phylogeny based on ITS, LSU, rbp2 and tub2 DNA sequences, revealed a new species here described as Hypoxylon guilanense. In addition, Hypoxylon rubiginosumsensu stricto was also encountered. Concurrently, an endophytic isolate of the latter species showed strong antagonistic activities against the Ash Dieback pathogen, Hymenoscyphus fraxineus, in a dual culture assay in our laboratory. Therefore, we decided to test the new Iranian fungi for antagonistic activities against the pathogen, along with several cultures of other Hypoxylon species that are related to H. rubiginosum. Our results suggest that the antagonistic effects of Hypoxylon spp. against Hym. fraxineus are widespread and that they are due to the production of antifungal phomopsidin derivatives in the presence of the pathogen.
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spelling pubmed-71953822020-05-06 Discovery of a new species of the Hypoxylon rubiginosum complex from Iran and antagonistic activities of Hypoxylon spp. against the Ash Dieback pathogen, Hymenoscyphus fraxineus, in dual culture Pourmoghaddam, Mohammad Javad Lambert, Christopher Surup, Frank Khodaparast, Seyed Akbar Krisai-Greilhuber, Irmgard Voglmayr, Hermann Stadler, Marc MycoKeys Research Article During a survey of xylarialean fungi in Northern Iran, several specimens that showed affinities to the Hypoxylon rubiginosum complex were collected and cultured. A comparison of their morphological characters, combined with a chemotaxonomic study based on high performance liquid chromatography, coupled with diode array detection and mass spectrometry (HPLC-DAD/MS) and a multi-locus phylogeny based on ITS, LSU, rbp2 and tub2 DNA sequences, revealed a new species here described as Hypoxylon guilanense. In addition, Hypoxylon rubiginosumsensu stricto was also encountered. Concurrently, an endophytic isolate of the latter species showed strong antagonistic activities against the Ash Dieback pathogen, Hymenoscyphus fraxineus, in a dual culture assay in our laboratory. Therefore, we decided to test the new Iranian fungi for antagonistic activities against the pathogen, along with several cultures of other Hypoxylon species that are related to H. rubiginosum. Our results suggest that the antagonistic effects of Hypoxylon spp. against Hym. fraxineus are widespread and that they are due to the production of antifungal phomopsidin derivatives in the presence of the pathogen. Pensoft Publishers 2020-04-24 /pmc/articles/PMC7195382/ /pubmed/32377154 http://dx.doi.org/10.3897/mycokeys.66.50946 Text en Mohammad Javad Pormoghadam, Christopher Lambert, Frank Surup, Seyed Akbar Khodaparast, Irmgard Krisai-Greilhuber, Hermann Voglmayr, Marc Stadler http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Pourmoghaddam, Mohammad Javad
Lambert, Christopher
Surup, Frank
Khodaparast, Seyed Akbar
Krisai-Greilhuber, Irmgard
Voglmayr, Hermann
Stadler, Marc
Discovery of a new species of the Hypoxylon rubiginosum complex from Iran and antagonistic activities of Hypoxylon spp. against the Ash Dieback pathogen, Hymenoscyphus fraxineus, in dual culture
title Discovery of a new species of the Hypoxylon rubiginosum complex from Iran and antagonistic activities of Hypoxylon spp. against the Ash Dieback pathogen, Hymenoscyphus fraxineus, in dual culture
title_full Discovery of a new species of the Hypoxylon rubiginosum complex from Iran and antagonistic activities of Hypoxylon spp. against the Ash Dieback pathogen, Hymenoscyphus fraxineus, in dual culture
title_fullStr Discovery of a new species of the Hypoxylon rubiginosum complex from Iran and antagonistic activities of Hypoxylon spp. against the Ash Dieback pathogen, Hymenoscyphus fraxineus, in dual culture
title_full_unstemmed Discovery of a new species of the Hypoxylon rubiginosum complex from Iran and antagonistic activities of Hypoxylon spp. against the Ash Dieback pathogen, Hymenoscyphus fraxineus, in dual culture
title_short Discovery of a new species of the Hypoxylon rubiginosum complex from Iran and antagonistic activities of Hypoxylon spp. against the Ash Dieback pathogen, Hymenoscyphus fraxineus, in dual culture
title_sort discovery of a new species of the hypoxylon rubiginosum complex from iran and antagonistic activities of hypoxylon spp. against the ash dieback pathogen, hymenoscyphus fraxineus, in dual culture
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7195382/
https://www.ncbi.nlm.nih.gov/pubmed/32377154
http://dx.doi.org/10.3897/mycokeys.66.50946
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