Lipid metabolism adaptations are reduced in human compared to murine Schwann cells following injury

Mammals differ in their regeneration potential after traumatic injury, which might be caused by species-specific regeneration programs. Here, we compared murine and human Schwann cell (SC) response to injury and developed an ex vivo injury model employing surgery-derived human sural nerves. Transcri...

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Autores principales: Meyer zu Reckendorf, Sofia, Brand, Christine, Pedro, Maria T., Hegler, Jutta, Schilling, Corinna S., Lerner, Raissa, Bindila, Laura, Antoniadis, Gregor, Knöll, Bernd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7195462/
https://www.ncbi.nlm.nih.gov/pubmed/32358558
http://dx.doi.org/10.1038/s41467-020-15915-4
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author Meyer zu Reckendorf, Sofia
Brand, Christine
Pedro, Maria T.
Hegler, Jutta
Schilling, Corinna S.
Lerner, Raissa
Bindila, Laura
Antoniadis, Gregor
Knöll, Bernd
author_facet Meyer zu Reckendorf, Sofia
Brand, Christine
Pedro, Maria T.
Hegler, Jutta
Schilling, Corinna S.
Lerner, Raissa
Bindila, Laura
Antoniadis, Gregor
Knöll, Bernd
author_sort Meyer zu Reckendorf, Sofia
collection PubMed
description Mammals differ in their regeneration potential after traumatic injury, which might be caused by species-specific regeneration programs. Here, we compared murine and human Schwann cell (SC) response to injury and developed an ex vivo injury model employing surgery-derived human sural nerves. Transcriptomic and lipid metabolism analysis of murine SCs following injury of sural nerves revealed down-regulation of lipogenic genes and regulator of lipid metabolism, including Pparg (peroxisome proliferator-activated receptor gamma) and S1P (sphingosine-1-phosphate). Human SCs failed to induce similar adaptations following ex vivo nerve injury. Pharmacological PPARg and S1P stimulation in mice resulted in up-regulation of lipid gene expression, suggesting a role in SCs switching towards a myelinating state. Altogether, our results suggest that murine SC switching towards a repair state is accompanied by transcriptome and lipidome adaptations, which are reduced in humans.
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spelling pubmed-71954622020-05-05 Lipid metabolism adaptations are reduced in human compared to murine Schwann cells following injury Meyer zu Reckendorf, Sofia Brand, Christine Pedro, Maria T. Hegler, Jutta Schilling, Corinna S. Lerner, Raissa Bindila, Laura Antoniadis, Gregor Knöll, Bernd Nat Commun Article Mammals differ in their regeneration potential after traumatic injury, which might be caused by species-specific regeneration programs. Here, we compared murine and human Schwann cell (SC) response to injury and developed an ex vivo injury model employing surgery-derived human sural nerves. Transcriptomic and lipid metabolism analysis of murine SCs following injury of sural nerves revealed down-regulation of lipogenic genes and regulator of lipid metabolism, including Pparg (peroxisome proliferator-activated receptor gamma) and S1P (sphingosine-1-phosphate). Human SCs failed to induce similar adaptations following ex vivo nerve injury. Pharmacological PPARg and S1P stimulation in mice resulted in up-regulation of lipid gene expression, suggesting a role in SCs switching towards a myelinating state. Altogether, our results suggest that murine SC switching towards a repair state is accompanied by transcriptome and lipidome adaptations, which are reduced in humans. Nature Publishing Group UK 2020-05-01 /pmc/articles/PMC7195462/ /pubmed/32358558 http://dx.doi.org/10.1038/s41467-020-15915-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Meyer zu Reckendorf, Sofia
Brand, Christine
Pedro, Maria T.
Hegler, Jutta
Schilling, Corinna S.
Lerner, Raissa
Bindila, Laura
Antoniadis, Gregor
Knöll, Bernd
Lipid metabolism adaptations are reduced in human compared to murine Schwann cells following injury
title Lipid metabolism adaptations are reduced in human compared to murine Schwann cells following injury
title_full Lipid metabolism adaptations are reduced in human compared to murine Schwann cells following injury
title_fullStr Lipid metabolism adaptations are reduced in human compared to murine Schwann cells following injury
title_full_unstemmed Lipid metabolism adaptations are reduced in human compared to murine Schwann cells following injury
title_short Lipid metabolism adaptations are reduced in human compared to murine Schwann cells following injury
title_sort lipid metabolism adaptations are reduced in human compared to murine schwann cells following injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7195462/
https://www.ncbi.nlm.nih.gov/pubmed/32358558
http://dx.doi.org/10.1038/s41467-020-15915-4
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