A pull-down assay using DNA/RNA-conjugated beads with a customized competition strategy: An effective approach to identify DNA/RNA binding proteins

: • The customized method makes the traditional pull-down assays more effective and efficient to identify DNA/RNA binding protein(s). • With the competitor of your choice, the method provides specific information about the competitive binding between DNA/RNA and binding proteins.

Detalles Bibliográficos
Autores principales: Sui, Hongyan, Chen, Qian, Imamichi, Tomozumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Immunology and Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7195543/
https://www.ncbi.nlm.nih.gov/pubmed/32373481
http://dx.doi.org/10.1016/j.mex.2020.100890
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author Sui, Hongyan
Chen, Qian
Imamichi, Tomozumi
author_facet Sui, Hongyan
Chen, Qian
Imamichi, Tomozumi
author_sort Sui, Hongyan
collection PubMed
description : • The customized method makes the traditional pull-down assays more effective and efficient to identify DNA/RNA binding protein(s). • With the competitor of your choice, the method provides specific information about the competitive binding between DNA/RNA and binding proteins.
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spelling pubmed-71955432020-05-05 A pull-down assay using DNA/RNA-conjugated beads with a customized competition strategy: An effective approach to identify DNA/RNA binding proteins Sui, Hongyan Chen, Qian Imamichi, Tomozumi MethodsX Immunology and Microbiology : • The customized method makes the traditional pull-down assays more effective and efficient to identify DNA/RNA binding protein(s). • With the competitor of your choice, the method provides specific information about the competitive binding between DNA/RNA and binding proteins. Elsevier 2020-04-13 /pmc/articles/PMC7195543/ /pubmed/32373481 http://dx.doi.org/10.1016/j.mex.2020.100890 Text en © 2020 The Author(s). Published by Elsevier B.V. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Immunology and Microbiology
Sui, Hongyan
Chen, Qian
Imamichi, Tomozumi
A pull-down assay using DNA/RNA-conjugated beads with a customized competition strategy: An effective approach to identify DNA/RNA binding proteins
title A pull-down assay using DNA/RNA-conjugated beads with a customized competition strategy: An effective approach to identify DNA/RNA binding proteins
title_full A pull-down assay using DNA/RNA-conjugated beads with a customized competition strategy: An effective approach to identify DNA/RNA binding proteins
title_fullStr A pull-down assay using DNA/RNA-conjugated beads with a customized competition strategy: An effective approach to identify DNA/RNA binding proteins
title_full_unstemmed A pull-down assay using DNA/RNA-conjugated beads with a customized competition strategy: An effective approach to identify DNA/RNA binding proteins
title_short A pull-down assay using DNA/RNA-conjugated beads with a customized competition strategy: An effective approach to identify DNA/RNA binding proteins
title_sort pull-down assay using dna/rna-conjugated beads with a customized competition strategy: an effective approach to identify dna/rna binding proteins
topic Immunology and Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7195543/
https://www.ncbi.nlm.nih.gov/pubmed/32373481
http://dx.doi.org/10.1016/j.mex.2020.100890
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