Cargando…
Quantitative Analysis of Six Phenolic Acids in Artemisia capillaris (Yinchen) by HPLC-DAD and Their Transformation Pathways in Decoction Preparation Process
We aimed to establish a quantitative analysis method of six constituents (5-caffeoylquinic acid, 3-caffeoylquinic acid, 4-caffeoylquinic acid, 1,3-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid) in Artemisia capillaris (Yinchen) and its decoction by using HPLC couple...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7195674/ https://www.ncbi.nlm.nih.gov/pubmed/32377441 http://dx.doi.org/10.1155/2020/8950324 |
_version_ | 1783528585611444224 |
---|---|
author | Tian, Fang Ruan, Qun-Jia Zhang, Ying Cao, Hui Ma, Zhi-Guo Zhou, Gai-Lian Wu, Meng-Hua |
author_facet | Tian, Fang Ruan, Qun-Jia Zhang, Ying Cao, Hui Ma, Zhi-Guo Zhou, Gai-Lian Wu, Meng-Hua |
author_sort | Tian, Fang |
collection | PubMed |
description | We aimed to establish a quantitative analysis method of six constituents (5-caffeoylquinic acid, 3-caffeoylquinic acid, 4-caffeoylquinic acid, 1,3-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid) in Artemisia capillaris (Yinchen) and its decoction by using HPLC coupled with DAD. Besides, the transformation paths of the six constituents were analyzed in decoction preparation processing. The analytical method was fully validated in terms of linearity, sensitivity, precision, repeatability, and recovery and applied to assess the transformation trend and quantitative analysis of the six constituents in Yinchen decoction. The contents of six constituents varied greatly in Yinchen herb and Yinchen decoction, and there were inextricable internal relationships between them. Presumably 3-caffeoylquinic acid was isomerized to generate 5-caffeoylquinic acid and 4-caffeoylquinic acid. Similarly, 1,3-dicaffeoylquinic acid and 3,4-dicaffeoylquinic acid were produced by isomerization of 4,5-dicaffeoylquinic acid. In conclusion, this study provides a chemical basis for quality control of Yinchen decoction, and the changes of selected markers in decoction could give us some novel perspectives to study the relationship between substances and drug efficacy. |
format | Online Article Text |
id | pubmed-7195674 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-71956742020-05-06 Quantitative Analysis of Six Phenolic Acids in Artemisia capillaris (Yinchen) by HPLC-DAD and Their Transformation Pathways in Decoction Preparation Process Tian, Fang Ruan, Qun-Jia Zhang, Ying Cao, Hui Ma, Zhi-Guo Zhou, Gai-Lian Wu, Meng-Hua J Anal Methods Chem Research Article We aimed to establish a quantitative analysis method of six constituents (5-caffeoylquinic acid, 3-caffeoylquinic acid, 4-caffeoylquinic acid, 1,3-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid) in Artemisia capillaris (Yinchen) and its decoction by using HPLC coupled with DAD. Besides, the transformation paths of the six constituents were analyzed in decoction preparation processing. The analytical method was fully validated in terms of linearity, sensitivity, precision, repeatability, and recovery and applied to assess the transformation trend and quantitative analysis of the six constituents in Yinchen decoction. The contents of six constituents varied greatly in Yinchen herb and Yinchen decoction, and there were inextricable internal relationships between them. Presumably 3-caffeoylquinic acid was isomerized to generate 5-caffeoylquinic acid and 4-caffeoylquinic acid. Similarly, 1,3-dicaffeoylquinic acid and 3,4-dicaffeoylquinic acid were produced by isomerization of 4,5-dicaffeoylquinic acid. In conclusion, this study provides a chemical basis for quality control of Yinchen decoction, and the changes of selected markers in decoction could give us some novel perspectives to study the relationship between substances and drug efficacy. Hindawi 2020-04-23 /pmc/articles/PMC7195674/ /pubmed/32377441 http://dx.doi.org/10.1155/2020/8950324 Text en Copyright © 2020 Fang Tian et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Tian, Fang Ruan, Qun-Jia Zhang, Ying Cao, Hui Ma, Zhi-Guo Zhou, Gai-Lian Wu, Meng-Hua Quantitative Analysis of Six Phenolic Acids in Artemisia capillaris (Yinchen) by HPLC-DAD and Their Transformation Pathways in Decoction Preparation Process |
title | Quantitative Analysis of Six Phenolic Acids in Artemisia capillaris (Yinchen) by HPLC-DAD and Their Transformation Pathways in Decoction Preparation Process |
title_full | Quantitative Analysis of Six Phenolic Acids in Artemisia capillaris (Yinchen) by HPLC-DAD and Their Transformation Pathways in Decoction Preparation Process |
title_fullStr | Quantitative Analysis of Six Phenolic Acids in Artemisia capillaris (Yinchen) by HPLC-DAD and Their Transformation Pathways in Decoction Preparation Process |
title_full_unstemmed | Quantitative Analysis of Six Phenolic Acids in Artemisia capillaris (Yinchen) by HPLC-DAD and Their Transformation Pathways in Decoction Preparation Process |
title_short | Quantitative Analysis of Six Phenolic Acids in Artemisia capillaris (Yinchen) by HPLC-DAD and Their Transformation Pathways in Decoction Preparation Process |
title_sort | quantitative analysis of six phenolic acids in artemisia capillaris (yinchen) by hplc-dad and their transformation pathways in decoction preparation process |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7195674/ https://www.ncbi.nlm.nih.gov/pubmed/32377441 http://dx.doi.org/10.1155/2020/8950324 |
work_keys_str_mv | AT tianfang quantitativeanalysisofsixphenolicacidsinartemisiacapillarisyinchenbyhplcdadandtheirtransformationpathwaysindecoctionpreparationprocess AT ruanqunjia quantitativeanalysisofsixphenolicacidsinartemisiacapillarisyinchenbyhplcdadandtheirtransformationpathwaysindecoctionpreparationprocess AT zhangying quantitativeanalysisofsixphenolicacidsinartemisiacapillarisyinchenbyhplcdadandtheirtransformationpathwaysindecoctionpreparationprocess AT caohui quantitativeanalysisofsixphenolicacidsinartemisiacapillarisyinchenbyhplcdadandtheirtransformationpathwaysindecoctionpreparationprocess AT mazhiguo quantitativeanalysisofsixphenolicacidsinartemisiacapillarisyinchenbyhplcdadandtheirtransformationpathwaysindecoctionpreparationprocess AT zhougailian quantitativeanalysisofsixphenolicacidsinartemisiacapillarisyinchenbyhplcdadandtheirtransformationpathwaysindecoctionpreparationprocess AT wumenghua quantitativeanalysisofsixphenolicacidsinartemisiacapillarisyinchenbyhplcdadandtheirtransformationpathwaysindecoctionpreparationprocess |