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Purification, characterization and antioxidant activities in vitro of polysaccharides from Amaranthus hybridus L.

BACKGROUND: Amaranthus hybridus L. is an annual, erect or less commonly ascending herb that is a member of the Amaranthaceae family. Polysaccharides extracted from traditional Chinese medicines may be effective substances with antioxidant activity. METHODS: In this study, we isolated crude polysacch...

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Autores principales: Tang, Zizhong, Zhou, Caixia, Cai, Yi, Tang, Yujia, Sun, Wenjun, Yao, Huipeng, Zheng, Tianrun, Chen, Hui, Xiao, Yirong, Shan, Zhi, Bu, Tongliang, Wang, Xiaoli, Huang, Lin, Gou, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7195838/
https://www.ncbi.nlm.nih.gov/pubmed/32391207
http://dx.doi.org/10.7717/peerj.9077
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author Tang, Zizhong
Zhou, Caixia
Cai, Yi
Tang, Yujia
Sun, Wenjun
Yao, Huipeng
Zheng, Tianrun
Chen, Hui
Xiao, Yirong
Shan, Zhi
Bu, Tongliang
Wang, Xiaoli
Huang, Lin
Gou, Lin
author_facet Tang, Zizhong
Zhou, Caixia
Cai, Yi
Tang, Yujia
Sun, Wenjun
Yao, Huipeng
Zheng, Tianrun
Chen, Hui
Xiao, Yirong
Shan, Zhi
Bu, Tongliang
Wang, Xiaoli
Huang, Lin
Gou, Lin
author_sort Tang, Zizhong
collection PubMed
description BACKGROUND: Amaranthus hybridus L. is an annual, erect or less commonly ascending herb that is a member of the Amaranthaceae family. Polysaccharides extracted from traditional Chinese medicines may be effective substances with antioxidant activity. METHODS: In this study, we isolated crude polysaccharides from A. hybridus (AHP-M) using microwave-assisted extraction. Then, the AHP-M was purified by chromatography with DEAE-32 cellulose, and two fractions, AHP-M-1 and AHP-M-2, were obtained. The structural characteristics of AHP-M-1 and AHP-M-2 were investigated, and their antioxidant activities were analyzed in vitro. RESULTS: We found that the monosaccharide composition of AHP-M-1 was different from that of AHP-M-2. The molecular weights of AHP-M-1 and AHP-M-2 were 77.625 kDa and 93.325 kDa, respectively. The results showed that the antioxidant activity of AHP-M-2 was better than that of AHP-M-1. For AHP-M-2, the DPPH radical scavenging rate at a concentration of 2 mg/mL was 78.87%, the hydroxyl radical scavenging rate was 39.34%, the superoxide anion radical scavenging rate was 80.2%, and the reduction ability of Fe(3+) was approximately 0.90. The total antioxidant capacity per milligram of AHP-M-2 was 6.42, which was higher than that of Vitamin C (Vc). CONCLUSION: The in vitro test indicated that AHP-M-1 and AHP-M-2 have good antioxidant activity, demonstrating that A. hybridus L. polysaccharide has immense potential as a natural antioxidants.
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spelling pubmed-71958382020-05-08 Purification, characterization and antioxidant activities in vitro of polysaccharides from Amaranthus hybridus L. Tang, Zizhong Zhou, Caixia Cai, Yi Tang, Yujia Sun, Wenjun Yao, Huipeng Zheng, Tianrun Chen, Hui Xiao, Yirong Shan, Zhi Bu, Tongliang Wang, Xiaoli Huang, Lin Gou, Lin PeerJ Biochemistry BACKGROUND: Amaranthus hybridus L. is an annual, erect or less commonly ascending herb that is a member of the Amaranthaceae family. Polysaccharides extracted from traditional Chinese medicines may be effective substances with antioxidant activity. METHODS: In this study, we isolated crude polysaccharides from A. hybridus (AHP-M) using microwave-assisted extraction. Then, the AHP-M was purified by chromatography with DEAE-32 cellulose, and two fractions, AHP-M-1 and AHP-M-2, were obtained. The structural characteristics of AHP-M-1 and AHP-M-2 were investigated, and their antioxidant activities were analyzed in vitro. RESULTS: We found that the monosaccharide composition of AHP-M-1 was different from that of AHP-M-2. The molecular weights of AHP-M-1 and AHP-M-2 were 77.625 kDa and 93.325 kDa, respectively. The results showed that the antioxidant activity of AHP-M-2 was better than that of AHP-M-1. For AHP-M-2, the DPPH radical scavenging rate at a concentration of 2 mg/mL was 78.87%, the hydroxyl radical scavenging rate was 39.34%, the superoxide anion radical scavenging rate was 80.2%, and the reduction ability of Fe(3+) was approximately 0.90. The total antioxidant capacity per milligram of AHP-M-2 was 6.42, which was higher than that of Vitamin C (Vc). CONCLUSION: The in vitro test indicated that AHP-M-1 and AHP-M-2 have good antioxidant activity, demonstrating that A. hybridus L. polysaccharide has immense potential as a natural antioxidants. PeerJ Inc. 2020-04-29 /pmc/articles/PMC7195838/ /pubmed/32391207 http://dx.doi.org/10.7717/peerj.9077 Text en © 2020 Tang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Biochemistry
Tang, Zizhong
Zhou, Caixia
Cai, Yi
Tang, Yujia
Sun, Wenjun
Yao, Huipeng
Zheng, Tianrun
Chen, Hui
Xiao, Yirong
Shan, Zhi
Bu, Tongliang
Wang, Xiaoli
Huang, Lin
Gou, Lin
Purification, characterization and antioxidant activities in vitro of polysaccharides from Amaranthus hybridus L.
title Purification, characterization and antioxidant activities in vitro of polysaccharides from Amaranthus hybridus L.
title_full Purification, characterization and antioxidant activities in vitro of polysaccharides from Amaranthus hybridus L.
title_fullStr Purification, characterization and antioxidant activities in vitro of polysaccharides from Amaranthus hybridus L.
title_full_unstemmed Purification, characterization and antioxidant activities in vitro of polysaccharides from Amaranthus hybridus L.
title_short Purification, characterization and antioxidant activities in vitro of polysaccharides from Amaranthus hybridus L.
title_sort purification, characterization and antioxidant activities in vitro of polysaccharides from amaranthus hybridus l.
topic Biochemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7195838/
https://www.ncbi.nlm.nih.gov/pubmed/32391207
http://dx.doi.org/10.7717/peerj.9077
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