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Long Noncoding RNA H19 Facilitates Small Cell Lung Cancer Tumorigenesis Through miR-140-5p/FGF9 Axis

PURPOSE: Long noncoding RNAs (lncRNAs) are emerging as gene regulators to drive many important cancer phenotypes through interaction with microRNAs. There have been numerous data about upregulation of H19 and its strong oncogenic function in progression of cancers. However, the function and detailed...

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Autores principales: Li, Xingkai, Lv, Fang, Li, Fang, Du, Minjun, Liang, Yicheng, Ju, Shaolong, Liu, Zixu, Wang, Bing, Gao, Yushun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7196199/
https://www.ncbi.nlm.nih.gov/pubmed/32425552
http://dx.doi.org/10.2147/OTT.S245710
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author Li, Xingkai
Lv, Fang
Li, Fang
Du, Minjun
Liang, Yicheng
Ju, Shaolong
Liu, Zixu
Wang, Bing
Gao, Yushun
author_facet Li, Xingkai
Lv, Fang
Li, Fang
Du, Minjun
Liang, Yicheng
Ju, Shaolong
Liu, Zixu
Wang, Bing
Gao, Yushun
author_sort Li, Xingkai
collection PubMed
description PURPOSE: Long noncoding RNAs (lncRNAs) are emerging as gene regulators to drive many important cancer phenotypes through interaction with microRNAs. There have been numerous data about upregulation of H19 and its strong oncogenic function in progression of cancers. However, the function and detailed mechanisms of H19 on small cell lung cancer (SCLC) are still unclear. METHODS: In this study, we investigated H19 expression in SCLC and para-carcinoma tissues. We also explored the function and detailed mechanisms of H19 on SCLC cells via RT-PCR, transwell assay, Western blot, dual-luciferase report assay and RNA pull-down experiments. RESULTS: In this study, we observed that H19 was upregulated in SCLC compared with para-carcinoma tissues or NSCLC tissues. We also uncovered that H19 could promote proliferation and migration of SCLC cells. Functional investigation illustrated that H19 acted as a sponge for miR-140-5p to regulate its expression in SCLC. Interestingly, we further found that H19 upregulated FGF9 expression to promote SCLC progression via sponging miR-140-5p. H19 and FGF9 were also revealed to have similar expression patterns in clinical SCLC samples. CONCLUSION: These data demonstrated that H19 might be a promising prognostic and therapeutic target for SCLC.
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spelling pubmed-71961992020-05-18 Long Noncoding RNA H19 Facilitates Small Cell Lung Cancer Tumorigenesis Through miR-140-5p/FGF9 Axis Li, Xingkai Lv, Fang Li, Fang Du, Minjun Liang, Yicheng Ju, Shaolong Liu, Zixu Wang, Bing Gao, Yushun Onco Targets Ther Original Research PURPOSE: Long noncoding RNAs (lncRNAs) are emerging as gene regulators to drive many important cancer phenotypes through interaction with microRNAs. There have been numerous data about upregulation of H19 and its strong oncogenic function in progression of cancers. However, the function and detailed mechanisms of H19 on small cell lung cancer (SCLC) are still unclear. METHODS: In this study, we investigated H19 expression in SCLC and para-carcinoma tissues. We also explored the function and detailed mechanisms of H19 on SCLC cells via RT-PCR, transwell assay, Western blot, dual-luciferase report assay and RNA pull-down experiments. RESULTS: In this study, we observed that H19 was upregulated in SCLC compared with para-carcinoma tissues or NSCLC tissues. We also uncovered that H19 could promote proliferation and migration of SCLC cells. Functional investigation illustrated that H19 acted as a sponge for miR-140-5p to regulate its expression in SCLC. Interestingly, we further found that H19 upregulated FGF9 expression to promote SCLC progression via sponging miR-140-5p. H19 and FGF9 were also revealed to have similar expression patterns in clinical SCLC samples. CONCLUSION: These data demonstrated that H19 might be a promising prognostic and therapeutic target for SCLC. Dove 2020-04-28 /pmc/articles/PMC7196199/ /pubmed/32425552 http://dx.doi.org/10.2147/OTT.S245710 Text en © 2020 Li et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Li, Xingkai
Lv, Fang
Li, Fang
Du, Minjun
Liang, Yicheng
Ju, Shaolong
Liu, Zixu
Wang, Bing
Gao, Yushun
Long Noncoding RNA H19 Facilitates Small Cell Lung Cancer Tumorigenesis Through miR-140-5p/FGF9 Axis
title Long Noncoding RNA H19 Facilitates Small Cell Lung Cancer Tumorigenesis Through miR-140-5p/FGF9 Axis
title_full Long Noncoding RNA H19 Facilitates Small Cell Lung Cancer Tumorigenesis Through miR-140-5p/FGF9 Axis
title_fullStr Long Noncoding RNA H19 Facilitates Small Cell Lung Cancer Tumorigenesis Through miR-140-5p/FGF9 Axis
title_full_unstemmed Long Noncoding RNA H19 Facilitates Small Cell Lung Cancer Tumorigenesis Through miR-140-5p/FGF9 Axis
title_short Long Noncoding RNA H19 Facilitates Small Cell Lung Cancer Tumorigenesis Through miR-140-5p/FGF9 Axis
title_sort long noncoding rna h19 facilitates small cell lung cancer tumorigenesis through mir-140-5p/fgf9 axis
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7196199/
https://www.ncbi.nlm.nih.gov/pubmed/32425552
http://dx.doi.org/10.2147/OTT.S245710
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