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An APN-activated NIR photosensitizer for cancer photodynamic therapy and fluorescence imaging

Photodynamic therapy has been developed as a prospective cancer treatment in recent years. Nevertheless, conventional photosensitizers suffer from lacking recognition and specificity to tumors, which causing severe side effects to normal tissues, while the enzyme-activated photosensitizers are capab...

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Autores principales: Zhou, Xiao, Li, Haidong, Shi, Chao, Xu, Feng, Zhang, Zhen, Yao, Qichao, Ma, He, Sun, Wen, Shao, Kun, Du, Jianjun, Long, Saran, Fan, Jiangli, Wang, Jingyun, Peng, Xiaojun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7196320/
https://www.ncbi.nlm.nih.gov/pubmed/32447103
http://dx.doi.org/10.1016/j.biomaterials.2020.120089
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author Zhou, Xiao
Li, Haidong
Shi, Chao
Xu, Feng
Zhang, Zhen
Yao, Qichao
Ma, He
Sun, Wen
Shao, Kun
Du, Jianjun
Long, Saran
Fan, Jiangli
Wang, Jingyun
Peng, Xiaojun
author_facet Zhou, Xiao
Li, Haidong
Shi, Chao
Xu, Feng
Zhang, Zhen
Yao, Qichao
Ma, He
Sun, Wen
Shao, Kun
Du, Jianjun
Long, Saran
Fan, Jiangli
Wang, Jingyun
Peng, Xiaojun
author_sort Zhou, Xiao
collection PubMed
description Photodynamic therapy has been developed as a prospective cancer treatment in recent years. Nevertheless, conventional photosensitizers suffer from lacking recognition and specificity to tumors, which causing severe side effects to normal tissues, while the enzyme-activated photosensitizers are capable of solving these conundrums due to high selectivity towards tumors. APN (Aminopeptidase N, APN/CD13), a tumor marker, has become a crucial targeting substance owing to its highly expressed on the cell membrane surface in various tumors, which has become a key point in the research of anti-tumor drug and fluorescence probe. Based on it, herein an APN-activated near-infrared (NIR) photosensitizer (APN-CyI) for tumor imaging and photodynamic therapy has been firstly developed and successfully applied in vitro and in vivo. Studies showed that APN-CyI could be activated by APN in tumor cells, hydrolyzed to fluorescent CyI-OH, which specifically located in mitochondria in cancer cells and exhibited a high singlet oxygen yield under NIR irradiation, and efficiently induced cancer cell apoptosis. Dramatically, the in vivo assays on Balb/c mice showed that APN-CyI could achieve NIR fluorescence imaging (λ(em) = 717 nm) for endogenous APN in tumors and possessed an efficient tumor suppression effect under NIR irradiation.
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spelling pubmed-71963202020-05-04 An APN-activated NIR photosensitizer for cancer photodynamic therapy and fluorescence imaging Zhou, Xiao Li, Haidong Shi, Chao Xu, Feng Zhang, Zhen Yao, Qichao Ma, He Sun, Wen Shao, Kun Du, Jianjun Long, Saran Fan, Jiangli Wang, Jingyun Peng, Xiaojun Biomaterials Article Photodynamic therapy has been developed as a prospective cancer treatment in recent years. Nevertheless, conventional photosensitizers suffer from lacking recognition and specificity to tumors, which causing severe side effects to normal tissues, while the enzyme-activated photosensitizers are capable of solving these conundrums due to high selectivity towards tumors. APN (Aminopeptidase N, APN/CD13), a tumor marker, has become a crucial targeting substance owing to its highly expressed on the cell membrane surface in various tumors, which has become a key point in the research of anti-tumor drug and fluorescence probe. Based on it, herein an APN-activated near-infrared (NIR) photosensitizer (APN-CyI) for tumor imaging and photodynamic therapy has been firstly developed and successfully applied in vitro and in vivo. Studies showed that APN-CyI could be activated by APN in tumor cells, hydrolyzed to fluorescent CyI-OH, which specifically located in mitochondria in cancer cells and exhibited a high singlet oxygen yield under NIR irradiation, and efficiently induced cancer cell apoptosis. Dramatically, the in vivo assays on Balb/c mice showed that APN-CyI could achieve NIR fluorescence imaging (λ(em) = 717 nm) for endogenous APN in tumors and possessed an efficient tumor suppression effect under NIR irradiation. Elsevier Ltd. 2020-09 2020-05-03 /pmc/articles/PMC7196320/ /pubmed/32447103 http://dx.doi.org/10.1016/j.biomaterials.2020.120089 Text en © 2020 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Zhou, Xiao
Li, Haidong
Shi, Chao
Xu, Feng
Zhang, Zhen
Yao, Qichao
Ma, He
Sun, Wen
Shao, Kun
Du, Jianjun
Long, Saran
Fan, Jiangli
Wang, Jingyun
Peng, Xiaojun
An APN-activated NIR photosensitizer for cancer photodynamic therapy and fluorescence imaging
title An APN-activated NIR photosensitizer for cancer photodynamic therapy and fluorescence imaging
title_full An APN-activated NIR photosensitizer for cancer photodynamic therapy and fluorescence imaging
title_fullStr An APN-activated NIR photosensitizer for cancer photodynamic therapy and fluorescence imaging
title_full_unstemmed An APN-activated NIR photosensitizer for cancer photodynamic therapy and fluorescence imaging
title_short An APN-activated NIR photosensitizer for cancer photodynamic therapy and fluorescence imaging
title_sort apn-activated nir photosensitizer for cancer photodynamic therapy and fluorescence imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7196320/
https://www.ncbi.nlm.nih.gov/pubmed/32447103
http://dx.doi.org/10.1016/j.biomaterials.2020.120089
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