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Effect of melatonin on bovine sperm characteristics and ultrastructure changes following cryopreservation
The production of reactive oxygen species (ROS) during cryopreservation of semen alters the sperm motion and mobility characteristics, resulting in poor or failure of conception rate after artificial insemination (AI). Melatonin an antioxidant is able to modulate the effect of ROS and prevents sperm...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7196679/ https://www.ncbi.nlm.nih.gov/pubmed/31797577 http://dx.doi.org/10.1002/vms3.224 |
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author | ChaithraShree, A. R. Ingole, Shailesh D. Dighe, Vikas D. Nagvekar, Anagha S. Bharucha, Simin V. Dagli, Nilesh R. Kekan, Prakash M. Kharde, Shambhudeo D. |
author_facet | ChaithraShree, A. R. Ingole, Shailesh D. Dighe, Vikas D. Nagvekar, Anagha S. Bharucha, Simin V. Dagli, Nilesh R. Kekan, Prakash M. Kharde, Shambhudeo D. |
author_sort | ChaithraShree, A. R. |
collection | PubMed |
description | The production of reactive oxygen species (ROS) during cryopreservation of semen alters the sperm motion and mobility characteristics, resulting in poor or failure of conception rate after artificial insemination (AI). Melatonin an antioxidant is able to modulate the effect of ROS and prevents spermatozoa by reducing the oxidative stress during freezing process. Eight ejaculates from eight healthy HF bulls diluted with Tris egg yolk glycerol extender were divided into five equal aliquots. The Computer Assisted Semen Analyser (CASA) results showed no significant difference between the control—post‐ thaw samples and melatonin‐treated samples; however, the velocity of spermatozoa with regard to the VAP, VSL showed highest increase in the 0.25 mM MLT‐treated semen followed by 0.1 mM MLT treated semen except for VCL where velocity increased with increase in the concentration of melatonin. The vigour of spermatozoa regard to BCF, STR and LIN recorded highest increase in the 0.25 mM MLT treated semen followed by 0.1 mM MLT‐treated semen except for the ALH where vigour increased with increase in the concentration of melatonin. The electron micrography images illustrated that the addition of 0.1 mM melatonin protected the plasma membrane and acrosome region and maintained the ultrastructure integrity of the cryopreserved spermatozoa when compared to control group, whereas the electron micrography of spermatozoa treated with 0.2 and 0.25 mM melatonin illustrated highest damage to the plasma and acrosome membrane. Thus concluding that inclusion of melatonin to sperm extender can improve the post‐thaw quality of cryopreserved bull spermatozoa. |
format | Online Article Text |
id | pubmed-7196679 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71966792020-05-05 Effect of melatonin on bovine sperm characteristics and ultrastructure changes following cryopreservation ChaithraShree, A. R. Ingole, Shailesh D. Dighe, Vikas D. Nagvekar, Anagha S. Bharucha, Simin V. Dagli, Nilesh R. Kekan, Prakash M. Kharde, Shambhudeo D. Vet Med Sci Original Articles The production of reactive oxygen species (ROS) during cryopreservation of semen alters the sperm motion and mobility characteristics, resulting in poor or failure of conception rate after artificial insemination (AI). Melatonin an antioxidant is able to modulate the effect of ROS and prevents spermatozoa by reducing the oxidative stress during freezing process. Eight ejaculates from eight healthy HF bulls diluted with Tris egg yolk glycerol extender were divided into five equal aliquots. The Computer Assisted Semen Analyser (CASA) results showed no significant difference between the control—post‐ thaw samples and melatonin‐treated samples; however, the velocity of spermatozoa with regard to the VAP, VSL showed highest increase in the 0.25 mM MLT‐treated semen followed by 0.1 mM MLT treated semen except for VCL where velocity increased with increase in the concentration of melatonin. The vigour of spermatozoa regard to BCF, STR and LIN recorded highest increase in the 0.25 mM MLT treated semen followed by 0.1 mM MLT‐treated semen except for the ALH where vigour increased with increase in the concentration of melatonin. The electron micrography images illustrated that the addition of 0.1 mM melatonin protected the plasma membrane and acrosome region and maintained the ultrastructure integrity of the cryopreserved spermatozoa when compared to control group, whereas the electron micrography of spermatozoa treated with 0.2 and 0.25 mM melatonin illustrated highest damage to the plasma and acrosome membrane. Thus concluding that inclusion of melatonin to sperm extender can improve the post‐thaw quality of cryopreserved bull spermatozoa. John Wiley and Sons Inc. 2019-12-02 /pmc/articles/PMC7196679/ /pubmed/31797577 http://dx.doi.org/10.1002/vms3.224 Text en © 2019 The Authors. Veterinary Medicine and Science Published by John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles ChaithraShree, A. R. Ingole, Shailesh D. Dighe, Vikas D. Nagvekar, Anagha S. Bharucha, Simin V. Dagli, Nilesh R. Kekan, Prakash M. Kharde, Shambhudeo D. Effect of melatonin on bovine sperm characteristics and ultrastructure changes following cryopreservation |
title | Effect of melatonin on bovine sperm characteristics and ultrastructure changes following cryopreservation |
title_full | Effect of melatonin on bovine sperm characteristics and ultrastructure changes following cryopreservation |
title_fullStr | Effect of melatonin on bovine sperm characteristics and ultrastructure changes following cryopreservation |
title_full_unstemmed | Effect of melatonin on bovine sperm characteristics and ultrastructure changes following cryopreservation |
title_short | Effect of melatonin on bovine sperm characteristics and ultrastructure changes following cryopreservation |
title_sort | effect of melatonin on bovine sperm characteristics and ultrastructure changes following cryopreservation |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7196679/ https://www.ncbi.nlm.nih.gov/pubmed/31797577 http://dx.doi.org/10.1002/vms3.224 |
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