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The First Egyptian Report Showing the Co-Existence of bla(NDM-25), bla(OXA-23), bla(OXA-181), and bla(GES-1) Among Carbapenem-Resistant K. pneumoniae Clinical Isolates Genotyped by BOX-PCR
BACKGROUND AND OBJECTIVE: The emergence of carbapenem-resistant K. pneumoniae (CRKP) continues to escalate and is alarming because of the emergence of pan drug-resistant strains. The objective of this study was to investigate the existence of 12 carbapenemase genes among CRKP clinical isolates. METH...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Dove
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7196799/ https://www.ncbi.nlm.nih.gov/pubmed/32425561 http://dx.doi.org/10.2147/IDR.S244064 |
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author | El-Badawy, Mohamed F El-Far, Shaymaa W Althobaiti, Shaker S Abou- Elazm, Fatma I Shohayeb, Mohamed M |
author_facet | El-Badawy, Mohamed F El-Far, Shaymaa W Althobaiti, Shaker S Abou- Elazm, Fatma I Shohayeb, Mohamed M |
author_sort | El-Badawy, Mohamed F |
collection | PubMed |
description | BACKGROUND AND OBJECTIVE: The emergence of carbapenem-resistant K. pneumoniae (CRKP) continues to escalate and is alarming because of the emergence of pan drug-resistant strains. The objective of this study was to investigate the existence of 12 carbapenemase genes among CRKP clinical isolates. METHODS: Ninety-six Klebsiella spp. clinical isolates were collected. The isolates were identified phenotypically and genotypically. These isolates were screened for susceptibility to 24 different antibiotics. The modified Hodge test (MHT) and the Carba Nordmann/Poirel (NP) test were used to phenotypically screen carbapenem-resistant strains for carbapenemase production. Phenotypic characterization of carbapenemases was performed using the combined disk synergy test (CDST). Additionally, the presence of 12 carbapenemase genes in CRKP isolates was investigated. The DNA sequence of bla(NDM) and bla(GES) genes was determined. The BOX-PCR technique was used to determine the clonal relationship between CRKP isolates. RESULTS: All carbapenem-resistant isolates were related to K. pneumoniae. Susceptibility testing showed that 19.79% (19/96) of the collected isolates were carbapenem-resistant. Of the CRKP isolates, 68.42% (13/19) tested positive for the MHT and Carba NP test. CDST showed that 42.11% (8/19), 63.16% (12/19), 47.37% (9/19), and 73.68% (14/19) of the CRKP isolates tested positive for the inhibitory effect of clavulanic acid, sulbactam, phenylboronic acid, and tazobactam, respectively, while 84.21% (16/19) and 68.42% (13/16) tested positive for the inhibitory effect of EDTA and mercaptopropionic acid, respectively. It was found that 10.53% (2/19) of the isolates tested positive for the inhibitory effect of sodium chloride. Molecular investigation of carbapenemases showed that 26.32% (5/19), 73.68% (14/19), 21.05% (4/19), 10.53% (2/19), and 5.26% (1/19) of the isolates tested positive for bla(NDM), bla(OXA-48), bla(OXA-181), bla(OXA-51), and bla(OXA-23), respectively. None of the isolates tested positive for bla(OXA-40) and bla(OXA-58). Two allelic variants of bla(NDM) (bla(NDM-1) and bla(NDM-25)) were detected. BOX-PCR revealed high clonal relatedness between CRKP isolates. CONCLUSION: MHT was more sensitive than Carba NP test for evaluating carbapenemase production and class D carbapenemase genes were the most prevalent of the 12 carbapenemase genes that were evaluated. |
format | Online Article Text |
id | pubmed-7196799 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-71967992020-05-18 The First Egyptian Report Showing the Co-Existence of bla(NDM-25), bla(OXA-23), bla(OXA-181), and bla(GES-1) Among Carbapenem-Resistant K. pneumoniae Clinical Isolates Genotyped by BOX-PCR El-Badawy, Mohamed F El-Far, Shaymaa W Althobaiti, Shaker S Abou- Elazm, Fatma I Shohayeb, Mohamed M Infect Drug Resist Original Research BACKGROUND AND OBJECTIVE: The emergence of carbapenem-resistant K. pneumoniae (CRKP) continues to escalate and is alarming because of the emergence of pan drug-resistant strains. The objective of this study was to investigate the existence of 12 carbapenemase genes among CRKP clinical isolates. METHODS: Ninety-six Klebsiella spp. clinical isolates were collected. The isolates were identified phenotypically and genotypically. These isolates were screened for susceptibility to 24 different antibiotics. The modified Hodge test (MHT) and the Carba Nordmann/Poirel (NP) test were used to phenotypically screen carbapenem-resistant strains for carbapenemase production. Phenotypic characterization of carbapenemases was performed using the combined disk synergy test (CDST). Additionally, the presence of 12 carbapenemase genes in CRKP isolates was investigated. The DNA sequence of bla(NDM) and bla(GES) genes was determined. The BOX-PCR technique was used to determine the clonal relationship between CRKP isolates. RESULTS: All carbapenem-resistant isolates were related to K. pneumoniae. Susceptibility testing showed that 19.79% (19/96) of the collected isolates were carbapenem-resistant. Of the CRKP isolates, 68.42% (13/19) tested positive for the MHT and Carba NP test. CDST showed that 42.11% (8/19), 63.16% (12/19), 47.37% (9/19), and 73.68% (14/19) of the CRKP isolates tested positive for the inhibitory effect of clavulanic acid, sulbactam, phenylboronic acid, and tazobactam, respectively, while 84.21% (16/19) and 68.42% (13/16) tested positive for the inhibitory effect of EDTA and mercaptopropionic acid, respectively. It was found that 10.53% (2/19) of the isolates tested positive for the inhibitory effect of sodium chloride. Molecular investigation of carbapenemases showed that 26.32% (5/19), 73.68% (14/19), 21.05% (4/19), 10.53% (2/19), and 5.26% (1/19) of the isolates tested positive for bla(NDM), bla(OXA-48), bla(OXA-181), bla(OXA-51), and bla(OXA-23), respectively. None of the isolates tested positive for bla(OXA-40) and bla(OXA-58). Two allelic variants of bla(NDM) (bla(NDM-1) and bla(NDM-25)) were detected. BOX-PCR revealed high clonal relatedness between CRKP isolates. CONCLUSION: MHT was more sensitive than Carba NP test for evaluating carbapenemase production and class D carbapenemase genes were the most prevalent of the 12 carbapenemase genes that were evaluated. Dove 2020-04-29 /pmc/articles/PMC7196799/ /pubmed/32425561 http://dx.doi.org/10.2147/IDR.S244064 Text en © 2020 El-Badawy et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research El-Badawy, Mohamed F El-Far, Shaymaa W Althobaiti, Shaker S Abou- Elazm, Fatma I Shohayeb, Mohamed M The First Egyptian Report Showing the Co-Existence of bla(NDM-25), bla(OXA-23), bla(OXA-181), and bla(GES-1) Among Carbapenem-Resistant K. pneumoniae Clinical Isolates Genotyped by BOX-PCR |
title | The First Egyptian Report Showing the Co-Existence of bla(NDM-25), bla(OXA-23), bla(OXA-181), and bla(GES-1) Among Carbapenem-Resistant K. pneumoniae Clinical Isolates Genotyped by BOX-PCR |
title_full | The First Egyptian Report Showing the Co-Existence of bla(NDM-25), bla(OXA-23), bla(OXA-181), and bla(GES-1) Among Carbapenem-Resistant K. pneumoniae Clinical Isolates Genotyped by BOX-PCR |
title_fullStr | The First Egyptian Report Showing the Co-Existence of bla(NDM-25), bla(OXA-23), bla(OXA-181), and bla(GES-1) Among Carbapenem-Resistant K. pneumoniae Clinical Isolates Genotyped by BOX-PCR |
title_full_unstemmed | The First Egyptian Report Showing the Co-Existence of bla(NDM-25), bla(OXA-23), bla(OXA-181), and bla(GES-1) Among Carbapenem-Resistant K. pneumoniae Clinical Isolates Genotyped by BOX-PCR |
title_short | The First Egyptian Report Showing the Co-Existence of bla(NDM-25), bla(OXA-23), bla(OXA-181), and bla(GES-1) Among Carbapenem-Resistant K. pneumoniae Clinical Isolates Genotyped by BOX-PCR |
title_sort | first egyptian report showing the co-existence of bla(ndm-25), bla(oxa-23), bla(oxa-181), and bla(ges-1) among carbapenem-resistant k. pneumoniae clinical isolates genotyped by box-pcr |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7196799/ https://www.ncbi.nlm.nih.gov/pubmed/32425561 http://dx.doi.org/10.2147/IDR.S244064 |
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