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Lack of CD8(+) T-cell co-localization with Kaposi’s sarcoma-associated herpesvirus infected cells in Kaposi’s sarcoma tumors

Despite the close association between Kaposi’s sarcoma (KS) and immune dysfunction, it remains unclear whether tumor infiltrating immune cells (TIIC), by their absence, presence, or dysfunction, are mechanistically correlated with KS pathogenesis. Therefore, their potential capacity to serve as prog...

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Autores principales: Lidenge, Salum J., Tso, For Yue, Ngalamika, Owen, Kolape, Jaydeep, Ngowi, John R., Mwaiselage, Julius, Wood, Charles, West, John T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7197452/
https://www.ncbi.nlm.nih.gov/pubmed/32391124
http://dx.doi.org/10.18632/oncotarget.27569
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author Lidenge, Salum J.
Tso, For Yue
Ngalamika, Owen
Kolape, Jaydeep
Ngowi, John R.
Mwaiselage, Julius
Wood, Charles
West, John T.
author_facet Lidenge, Salum J.
Tso, For Yue
Ngalamika, Owen
Kolape, Jaydeep
Ngowi, John R.
Mwaiselage, Julius
Wood, Charles
West, John T.
author_sort Lidenge, Salum J.
collection PubMed
description Despite the close association between Kaposi’s sarcoma (KS) and immune dysfunction, it remains unclear whether tumor infiltrating immune cells (TIIC), by their absence, presence, or dysfunction, are mechanistically correlated with KS pathogenesis. Therefore, their potential capacity to serve as prognostic biomarkers of KS disease progression or control is unclear. Because epidemic-KS (EpKS) occurs with HIV-1 co-infection, it is particularly important to compare TIIC between EpKS and HIV-negative African endemic-KS (EnKS) to dissect the roles of HIV-1 and Kaposi Sarcoma-associated herpesvirus (KSHV) in KS pathogenesis. This cross-sectional study of 13 advanced KS (4 EnKS, 9 EpKS) patients and 3 healthy controls utilized single-color immunohistochemistry and dual-color immunofluorescence assays to characterize and quantify KSHV infected cells in relation to various TIIC in KS biopsies. Analysis of variance (ANOVA) and Mann-Whitney tests were used to assess differences between groups where P-values < 0.05 were considered significant. The abundance of KSHV infected cells was heterogeneous in KS biopsies. Despite the presence of T-cell chemoattractant chemokine CxCL-9 in biopsies, CD8(+) T-cells were sparsely distributed in regions with evident KSHV infected cells but were readily detectable in regions devoid of KSHV infected cells (P < 0.0001). CD68(+) (M1) macrophages were evenly and diffusely distributed in KS biopsies, whereas, the majority of CD163(+) (M2) macrophages were localized in regions devoid of KSHV infected cells (P < 0.0001). Overall, the poor immune cell infiltration or co-localization in KS biopsies independent of HIV-1 co-infection suggests a fundamental tumor immune evasion mechanism that warrants further investigation.
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spelling pubmed-71974522020-05-08 Lack of CD8(+) T-cell co-localization with Kaposi’s sarcoma-associated herpesvirus infected cells in Kaposi’s sarcoma tumors Lidenge, Salum J. Tso, For Yue Ngalamika, Owen Kolape, Jaydeep Ngowi, John R. Mwaiselage, Julius Wood, Charles West, John T. Oncotarget Research Paper Despite the close association between Kaposi’s sarcoma (KS) and immune dysfunction, it remains unclear whether tumor infiltrating immune cells (TIIC), by their absence, presence, or dysfunction, are mechanistically correlated with KS pathogenesis. Therefore, their potential capacity to serve as prognostic biomarkers of KS disease progression or control is unclear. Because epidemic-KS (EpKS) occurs with HIV-1 co-infection, it is particularly important to compare TIIC between EpKS and HIV-negative African endemic-KS (EnKS) to dissect the roles of HIV-1 and Kaposi Sarcoma-associated herpesvirus (KSHV) in KS pathogenesis. This cross-sectional study of 13 advanced KS (4 EnKS, 9 EpKS) patients and 3 healthy controls utilized single-color immunohistochemistry and dual-color immunofluorescence assays to characterize and quantify KSHV infected cells in relation to various TIIC in KS biopsies. Analysis of variance (ANOVA) and Mann-Whitney tests were used to assess differences between groups where P-values < 0.05 were considered significant. The abundance of KSHV infected cells was heterogeneous in KS biopsies. Despite the presence of T-cell chemoattractant chemokine CxCL-9 in biopsies, CD8(+) T-cells were sparsely distributed in regions with evident KSHV infected cells but were readily detectable in regions devoid of KSHV infected cells (P < 0.0001). CD68(+) (M1) macrophages were evenly and diffusely distributed in KS biopsies, whereas, the majority of CD163(+) (M2) macrophages were localized in regions devoid of KSHV infected cells (P < 0.0001). Overall, the poor immune cell infiltration or co-localization in KS biopsies independent of HIV-1 co-infection suggests a fundamental tumor immune evasion mechanism that warrants further investigation. Impact Journals LLC 2020-04-28 /pmc/articles/PMC7197452/ /pubmed/32391124 http://dx.doi.org/10.18632/oncotarget.27569 Text en http://creativecommons.org/licenses/by/3.0/ Copyright: Lidenge et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Lidenge, Salum J.
Tso, For Yue
Ngalamika, Owen
Kolape, Jaydeep
Ngowi, John R.
Mwaiselage, Julius
Wood, Charles
West, John T.
Lack of CD8(+) T-cell co-localization with Kaposi’s sarcoma-associated herpesvirus infected cells in Kaposi’s sarcoma tumors
title Lack of CD8(+) T-cell co-localization with Kaposi’s sarcoma-associated herpesvirus infected cells in Kaposi’s sarcoma tumors
title_full Lack of CD8(+) T-cell co-localization with Kaposi’s sarcoma-associated herpesvirus infected cells in Kaposi’s sarcoma tumors
title_fullStr Lack of CD8(+) T-cell co-localization with Kaposi’s sarcoma-associated herpesvirus infected cells in Kaposi’s sarcoma tumors
title_full_unstemmed Lack of CD8(+) T-cell co-localization with Kaposi’s sarcoma-associated herpesvirus infected cells in Kaposi’s sarcoma tumors
title_short Lack of CD8(+) T-cell co-localization with Kaposi’s sarcoma-associated herpesvirus infected cells in Kaposi’s sarcoma tumors
title_sort lack of cd8(+) t-cell co-localization with kaposi’s sarcoma-associated herpesvirus infected cells in kaposi’s sarcoma tumors
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7197452/
https://www.ncbi.nlm.nih.gov/pubmed/32391124
http://dx.doi.org/10.18632/oncotarget.27569
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