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A genetic variant in microRNA-146a is associated with sporadic breast cancer in a Southern Brazilian Population

MicroRNAs (miRNAs) play an essential role in gene expression and affect the development of tumours, including breast cancer (BC). Polymorphisms in miRNA genes can affect the interaction of miRNAs with their target messenger RNA by interfering, creating or disrupting target sites. The single nucleoti...

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Autores principales: Brincas, Heloisa Magagnin, Augusto, Danillo G., Mathias, Carolina, Cavalli, Iglenir João, de Lima, Rubens Silveira, Kuroda, Flávia, Urban, Cícero de Andrade, Gradia, Daniela Fiori, de Oliveira, Jaqueline, de Almeida, Rodrigo Coutinho, Ribeiro, Enilze Maria de Souza Fonseca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Genética 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7198002/
https://www.ncbi.nlm.nih.gov/pubmed/32142098
http://dx.doi.org/10.1590/1678-4685-GMB-2019-0278
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author Brincas, Heloisa Magagnin
Augusto, Danillo G.
Mathias, Carolina
Cavalli, Iglenir João
de Lima, Rubens Silveira
Kuroda, Flávia
Urban, Cícero de Andrade
Gradia, Daniela Fiori
de Oliveira, Jaqueline
de Almeida, Rodrigo Coutinho
Ribeiro, Enilze Maria de Souza Fonseca
author_facet Brincas, Heloisa Magagnin
Augusto, Danillo G.
Mathias, Carolina
Cavalli, Iglenir João
de Lima, Rubens Silveira
Kuroda, Flávia
Urban, Cícero de Andrade
Gradia, Daniela Fiori
de Oliveira, Jaqueline
de Almeida, Rodrigo Coutinho
Ribeiro, Enilze Maria de Souza Fonseca
author_sort Brincas, Heloisa Magagnin
collection PubMed
description MicroRNAs (miRNAs) play an essential role in gene expression and affect the development of tumours, including breast cancer (BC). Polymorphisms in miRNA genes can affect the interaction of miRNAs with their target messenger RNA by interfering, creating or disrupting target sites. The single nucleotide polymorphism (SNP) rs2910164, located in the seed region of miR146a, was shown to be associated with BC among different populations. In the present study, we investigated whether rs2910164 is associated with BC in 326 patients and 411 controls from a Brazilian population of predominantly European ancestry. The presence of the allele rs2910164*C was associated with an increased risk of BC (OR=1.4, 95% CI=1.03-1.85, p = 0.03). We also analysed publicly available RNA-seq data to evaluate if miR146a is differentially expressed in different subtypes of BC. Genotyping was performed by polymerase chain reaction with sequence-specific primers (PCR-SSP). By leveraging public data from TCGA database, we analysed 461 patients and found that miR146a is significantly more expressed in BC than in non-tumor tissue (1.47 fold, p = 0.02) and is expressed to a greater degree in aggressive BC subtypes.
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spelling pubmed-71980022020-05-08 A genetic variant in microRNA-146a is associated with sporadic breast cancer in a Southern Brazilian Population Brincas, Heloisa Magagnin Augusto, Danillo G. Mathias, Carolina Cavalli, Iglenir João de Lima, Rubens Silveira Kuroda, Flávia Urban, Cícero de Andrade Gradia, Daniela Fiori de Oliveira, Jaqueline de Almeida, Rodrigo Coutinho Ribeiro, Enilze Maria de Souza Fonseca Genet Mol Biol Human and Medical Genetics MicroRNAs (miRNAs) play an essential role in gene expression and affect the development of tumours, including breast cancer (BC). Polymorphisms in miRNA genes can affect the interaction of miRNAs with their target messenger RNA by interfering, creating or disrupting target sites. The single nucleotide polymorphism (SNP) rs2910164, located in the seed region of miR146a, was shown to be associated with BC among different populations. In the present study, we investigated whether rs2910164 is associated with BC in 326 patients and 411 controls from a Brazilian population of predominantly European ancestry. The presence of the allele rs2910164*C was associated with an increased risk of BC (OR=1.4, 95% CI=1.03-1.85, p = 0.03). We also analysed publicly available RNA-seq data to evaluate if miR146a is differentially expressed in different subtypes of BC. Genotyping was performed by polymerase chain reaction with sequence-specific primers (PCR-SSP). By leveraging public data from TCGA database, we analysed 461 patients and found that miR146a is significantly more expressed in BC than in non-tumor tissue (1.47 fold, p = 0.02) and is expressed to a greater degree in aggressive BC subtypes. Sociedade Brasileira de Genética 2020-02-03 /pmc/articles/PMC7198002/ /pubmed/32142098 http://dx.doi.org/10.1590/1678-4685-GMB-2019-0278 Text en Copyright © 2019, Sociedade Brasileira de Genética. https://creativecommons.org/licenses/by/4.0/ License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License (type CC-BY), which permits unrestricted use, distribution and reproduction in any medium, provided the original article is properly cited.
spellingShingle Human and Medical Genetics
Brincas, Heloisa Magagnin
Augusto, Danillo G.
Mathias, Carolina
Cavalli, Iglenir João
de Lima, Rubens Silveira
Kuroda, Flávia
Urban, Cícero de Andrade
Gradia, Daniela Fiori
de Oliveira, Jaqueline
de Almeida, Rodrigo Coutinho
Ribeiro, Enilze Maria de Souza Fonseca
A genetic variant in microRNA-146a is associated with sporadic breast cancer in a Southern Brazilian Population
title A genetic variant in microRNA-146a is associated with sporadic breast cancer in a Southern Brazilian Population
title_full A genetic variant in microRNA-146a is associated with sporadic breast cancer in a Southern Brazilian Population
title_fullStr A genetic variant in microRNA-146a is associated with sporadic breast cancer in a Southern Brazilian Population
title_full_unstemmed A genetic variant in microRNA-146a is associated with sporadic breast cancer in a Southern Brazilian Population
title_short A genetic variant in microRNA-146a is associated with sporadic breast cancer in a Southern Brazilian Population
title_sort genetic variant in microrna-146a is associated with sporadic breast cancer in a southern brazilian population
topic Human and Medical Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7198002/
https://www.ncbi.nlm.nih.gov/pubmed/32142098
http://dx.doi.org/10.1590/1678-4685-GMB-2019-0278
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