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Transcriptome analyses of Ditylenchus destructor in responses to cold and desiccation stress

The objective of this study was to identify molecular responses in Ditylenchus destructor to cold and desiccation by means of transcriptomes analyses. A total of 102,517 unigenes were obtained, with an average length of 1,076 bp, in which 58,453 (57%) had a functional annotation. A total of 1154 sim...

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Autores principales: Ma, Juan, Gao, Bo, Wang, Rongyan, Li, Xiuhua, Chen, Shulong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Genética 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7198036/
https://www.ncbi.nlm.nih.gov/pubmed/32232317
http://dx.doi.org/10.1590/1678-4685-GMB-2018-0057
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author Ma, Juan
Gao, Bo
Wang, Rongyan
Li, Xiuhua
Chen, Shulong
author_facet Ma, Juan
Gao, Bo
Wang, Rongyan
Li, Xiuhua
Chen, Shulong
author_sort Ma, Juan
collection PubMed
description The objective of this study was to identify molecular responses in Ditylenchus destructor to cold and desiccation by means of transcriptomes analyses. A total of 102,517 unigenes were obtained, with an average length of 1,076 bp, in which 58,453 (57%) had a functional annotation. A total of 1154 simple sequence repeats (SSRs) distributed over 1078 unigenes were detected. Gene expression profiles in response to cold and desiccation stress and the expression of specific stress-related genes were compared. Gene ontology analysis and pathway-based analysis were used to further investigate the functions of the differentially expressed genes. The reliability of the sequencing data was verified through quantitative real-time PCR analysis of 19 stress-related genes. RNA interference used to further assess the functions of the cold-related unigenes 15628 and 15596 showed that the knockdown of each of these genes led to decreased cold tolerance of D. destructor. Hence, this study revealed molecular processes and pathways active in cold- or dessication-treated nematodes. The transcriptome profiles presented in this study provide insight into the transcriptome complexity and will contribute to further understand stress tolerance in D. destructor.
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spelling pubmed-71980362020-05-08 Transcriptome analyses of Ditylenchus destructor in responses to cold and desiccation stress Ma, Juan Gao, Bo Wang, Rongyan Li, Xiuhua Chen, Shulong Genet Mol Biol Animal Genetics The objective of this study was to identify molecular responses in Ditylenchus destructor to cold and desiccation by means of transcriptomes analyses. A total of 102,517 unigenes were obtained, with an average length of 1,076 bp, in which 58,453 (57%) had a functional annotation. A total of 1154 simple sequence repeats (SSRs) distributed over 1078 unigenes were detected. Gene expression profiles in response to cold and desiccation stress and the expression of specific stress-related genes were compared. Gene ontology analysis and pathway-based analysis were used to further investigate the functions of the differentially expressed genes. The reliability of the sequencing data was verified through quantitative real-time PCR analysis of 19 stress-related genes. RNA interference used to further assess the functions of the cold-related unigenes 15628 and 15596 showed that the knockdown of each of these genes led to decreased cold tolerance of D. destructor. Hence, this study revealed molecular processes and pathways active in cold- or dessication-treated nematodes. The transcriptome profiles presented in this study provide insight into the transcriptome complexity and will contribute to further understand stress tolerance in D. destructor. Sociedade Brasileira de Genética 2020-03-23 /pmc/articles/PMC7198036/ /pubmed/32232317 http://dx.doi.org/10.1590/1678-4685-GMB-2018-0057 Text en Copyright © 2020, Sociedade Brasileira de Genética. https://creativecommons.org/licenses/by/4.0/ License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License (type CC-BY), which permits unrestricted use, distribution and reproduction in any medium, provided the original article is properly cited.
spellingShingle Animal Genetics
Ma, Juan
Gao, Bo
Wang, Rongyan
Li, Xiuhua
Chen, Shulong
Transcriptome analyses of Ditylenchus destructor in responses to cold and desiccation stress
title Transcriptome analyses of Ditylenchus destructor in responses to cold and desiccation stress
title_full Transcriptome analyses of Ditylenchus destructor in responses to cold and desiccation stress
title_fullStr Transcriptome analyses of Ditylenchus destructor in responses to cold and desiccation stress
title_full_unstemmed Transcriptome analyses of Ditylenchus destructor in responses to cold and desiccation stress
title_short Transcriptome analyses of Ditylenchus destructor in responses to cold and desiccation stress
title_sort transcriptome analyses of ditylenchus destructor in responses to cold and desiccation stress
topic Animal Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7198036/
https://www.ncbi.nlm.nih.gov/pubmed/32232317
http://dx.doi.org/10.1590/1678-4685-GMB-2018-0057
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