Cargando…

The impacts of viral inactivating methods on quantitative RT-PCR for COVID-19

OBJECTIVE: This paper aims to explore the effect of four virus inactivation methods on the rapid detection results of COVID-19 nucleic acid. METHODS: Collected samples of nasopharyngeal swabs from 2 patients diagnosed with COVID-19 at the First People's Hospital of Zhaoqing City, each of sample...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wang, Yueying, Song, Wei, Zhao, Zuguo, Chen, Ping, Liu, Jian, Li, Chende
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier B.V. 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7198166/ https://www.ncbi.nlm.nih.gov/pubmed/32380210 http://dx.doi.org/10.1016/j.virusres.2020.197988

Ejemplares similares

Clinical COVID-19 diagnostic methods: Comparison of reverse transcription loop-mediated isothermal amplification (RT-LAMP) and quantitative RT-PCR (qRT-PCR)
por: Kitajima, Heita, et al.
Publicado: (2021)

Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection
por: Løvoll, Marie, et al.
Publicado: (2011)

RT-PCR negative COVID-19
por: Parmar, Heta, et al.
Publicado: (2022)

Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR
por: Jarošová, Jana, et al.
Publicado: (2010)

Viral load quantitation of SARS-coronavirus RNA using a one-step real-time RT-PCR
por: Keyaerts, Els, et al.
Publicado: (2006)

Evaluation of viral concentration and extraction methods for SARS-CoV-2 recovery from wastewater using droplet digital and quantitative RT-PCR
por: Dimitrakopoulos, Lampros, et al.
Publicado: (2022)

Selection of reference genes for quantitative RT-PCR (RT-qPCR) analysis of rat tissues under physiological and toxicological conditions
por: Svingen, Terje, et al.
Publicado: (2015)

Extraction-Free Rapid Cycle Quantitative RT-PCR and Extreme RT-PCR for SARS-CoV-2 Virus Detection
por: Lownik, Joseph C., et al.
Publicado: (2021)

Selection of optimal reference genes for normalization in quantitative RT-PCR
por: Chervoneva, Inna, et al.
Publicado: (2010)

Pre-amplification as a method for improvement of quantitative RT-PCR analysis of circulating miRNAs
por: Sekovanić, Ankica, et al.
Publicado: (2020)

Impact of metal ions on PCR inhibition and RT-PCR efficiency
por: Kuffel, Agnieszka, et al.
Publicado: (2020)

A simple, safe and sensitive method for SARS‐CoV‐2 inactivation and RNA extraction for RT‐qPCR
por: Kalnina, Lelde, et al.
Publicado: (2021)

Ex vivo screening for immunodominant viral epitopes by quantitative real time polymerase chain reaction (qRT-PCR)
por: Provenzano, Maurizio, et al.
Publicado: (2003)

Quantitative Evaluation and Selection of Reference Genes for Quantitative RT-PCR in Mouse Acute Pancreatitis
por: Yan, Zhaoping, et al.
Publicado: (2016)

Viral Dynamics and Real-Time RT-PCR Ct Values Correlation with Disease Severity in COVID-19
por: Rabaan, Ali A., et al.
Publicado: (2021)

Combination of CT and RT-PCR in the screening or diagnosis of COVID-19
por: Wang, Youxin, et al.
Publicado: (2020)

Reverse transcription-quantitative PCR (RT-qPCR) without the need for prior removal of DNA
por: Đermić, Damir, et al.
Publicado: (2023)

Chest CT of COVID-19 in patients with a negative first RT-PCR test: Comparison with patients with a positive first RT-PCR test
por: Chen, Zu-Hua, et al.
Publicado: (2020)

Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers
por: Balcells, Ingrid, et al.
Publicado: (2011)

Data-driven normalization strategies for high-throughput quantitative RT-PCR
por: Mar, Jessica C, et al.
Publicado: (2009)

Identification and validation of reference genes for quantitative RT-PCR normalization in wheat
por: Paolacci, Anna R, et al.
Publicado: (2009)

Identification of Reference Genes for Quantitative RT-PCR in Ascending Aortic Aneurysms
por: Henn, Dominic, et al.
Publicado: (2013)

RT-PCR for SARS-CoV-2: quantitative versus qualitative
por: Han, Mi Seon, et al.
Publicado: (2021)

COVID-19 Real-Time RT-PCR: Does Positivity on Follow-up RT-PCR Always Imply Infectivity?
por: Suri, Tejas, et al.
Publicado: (2020)

Comparison of chest computed tomography findings of RT-PCR negative and RT-PCR positive cases in COVID-19 patients
por: Özkarafakılı, Müfide Arzu, et al.
Publicado: (2022)

Evaluation of reference genes in mouse preimplantation embryos for gene expression studies using real-time quantitative RT-PCR (RT-qPCR)
por: Jeong, Jae-Kyo, et al.
Publicado: (2014)

Comparison of Reverse Transcription (RT)-Quantitative PCR and RT-Droplet Digital PCR for Detection of Genomic and Subgenomic SARS-CoV-2 RNA
por: Morón-López, Sara, et al.
Publicado: (2023)

Systematic Comparison and Validation of Quantitative Real-Time PCR Methods for the Quantitation of Adeno-Associated Viral Products
por: Werling, Natalie Jayne, et al.
Publicado: (2015)

Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
por: Paiva, Marcelo Henrique Santos, et al.
Publicado: (2017)

Comparison of the Rapid Antigen Testing Method With RT-qPCR for the Diagnosis of COVID-19
por: Pandey, Ashok Kumar, et al.
Publicado: (2021)

Analytical Performance of COVID-19 Detection Methods (RT-PCR): Scientific and Societal Concerns †
por: Verna, Roberto, et al.
Publicado: (2021)

Impact of the Nucleic Acid Extraction Method and the RT-qPCR Assay on SARS-CoV-2 Detection in Low-Viral Samples
por: Komiazyk, Magdalena, et al.
Publicado: (2021)

Quantification of bacteria by in vivo bioluminescence imaging in comparison with standard spread plate method and reverse transcription quantitative PCR (RT-qPCR)
por: Briestenská, Katarína, et al.
Publicado: (2021)

Corrigendum to “Quantitative Evaluation and Selection of Reference Genes for Quantitative RT-PCR in Mouse Acute Pancreatitis”
por: Yan, Zhaoping, et al.
Publicado: (2017)

Heat inactivation decreases the qualitative real-time RT-PCR detection rates of clinical samples with high cycle threshold values in COVID-19
por: Zou, Jingbo, et al.
Publicado: (2020)

Quantitative RT-PCR Gene Evaluation and RNA Interference in the Brown Marmorated Stink Bug
por: Bansal, Raman, et al.
Publicado: (2016)

Diagnostic performance of the Qiaprep amp Viral RNA UM kit for the detection of COVID-19 compared to RT-PCR
por: Becerril Vargas, Eduardo, et al.
Publicado: (2022)

Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR
por: Dorlass, Erick Gustavo, et al.
Publicado: (2020)

A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors
por: Poon, Leo L.M, et al.
Publicado: (2004)

Is there evidence of intra-uterine vertical transmission potential of COVID-19 infection in samples tested by quantitative RT-PCR?
por: Cheruiyot, Isaac, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_8i7024685a61d1bk4nt1n6davf