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Spatiotemporally precise optogenetic activation of sensory neurons in freely walking Drosophila
Previous work has characterized how walking Drosophila coordinate the movements of individual limbs (DeAngelis et al., 2019). To understand the circuit basis of this coordination, one must characterize how sensory feedback from each limb affects walking behavior. However, it has remained difficult t...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7198233/ https://www.ncbi.nlm.nih.gov/pubmed/32319425 http://dx.doi.org/10.7554/eLife.54183 |
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author | DeAngelis, Brian D Zavatone-Veth, Jacob A Gonzalez-Suarez, Aneysis D Clark, Damon A |
author_facet | DeAngelis, Brian D Zavatone-Veth, Jacob A Gonzalez-Suarez, Aneysis D Clark, Damon A |
author_sort | DeAngelis, Brian D |
collection | PubMed |
description | Previous work has characterized how walking Drosophila coordinate the movements of individual limbs (DeAngelis et al., 2019). To understand the circuit basis of this coordination, one must characterize how sensory feedback from each limb affects walking behavior. However, it has remained difficult to manipulate neural activity in individual limbs of freely moving animals. Here, we demonstrate a simple method for optogenetic stimulation with body side-, body segment-, and limb-specificity that does not require real-time tracking. Instead, we activate at random, precise locations in time and space and use post hoc analysis to determine behavioral responses to specific activations. Using this method, we have characterized limb coordination and walking behavior in response to transient activation of mechanosensitive bristle neurons and sweet-sensing chemoreceptor neurons. Our findings reveal that activating these neurons has opposite effects on turning, and that activations in different limbs and body regions produce distinct behaviors. |
format | Online Article Text |
id | pubmed-7198233 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-71982332020-05-06 Spatiotemporally precise optogenetic activation of sensory neurons in freely walking Drosophila DeAngelis, Brian D Zavatone-Veth, Jacob A Gonzalez-Suarez, Aneysis D Clark, Damon A eLife Neuroscience Previous work has characterized how walking Drosophila coordinate the movements of individual limbs (DeAngelis et al., 2019). To understand the circuit basis of this coordination, one must characterize how sensory feedback from each limb affects walking behavior. However, it has remained difficult to manipulate neural activity in individual limbs of freely moving animals. Here, we demonstrate a simple method for optogenetic stimulation with body side-, body segment-, and limb-specificity that does not require real-time tracking. Instead, we activate at random, precise locations in time and space and use post hoc analysis to determine behavioral responses to specific activations. Using this method, we have characterized limb coordination and walking behavior in response to transient activation of mechanosensitive bristle neurons and sweet-sensing chemoreceptor neurons. Our findings reveal that activating these neurons has opposite effects on turning, and that activations in different limbs and body regions produce distinct behaviors. eLife Sciences Publications, Ltd 2020-04-22 /pmc/articles/PMC7198233/ /pubmed/32319425 http://dx.doi.org/10.7554/eLife.54183 Text en © 2020, DeAngelis et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience DeAngelis, Brian D Zavatone-Veth, Jacob A Gonzalez-Suarez, Aneysis D Clark, Damon A Spatiotemporally precise optogenetic activation of sensory neurons in freely walking Drosophila |
title | Spatiotemporally precise optogenetic activation of sensory neurons in freely walking Drosophila |
title_full | Spatiotemporally precise optogenetic activation of sensory neurons in freely walking Drosophila |
title_fullStr | Spatiotemporally precise optogenetic activation of sensory neurons in freely walking Drosophila |
title_full_unstemmed | Spatiotemporally precise optogenetic activation of sensory neurons in freely walking Drosophila |
title_short | Spatiotemporally precise optogenetic activation of sensory neurons in freely walking Drosophila |
title_sort | spatiotemporally precise optogenetic activation of sensory neurons in freely walking drosophila |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7198233/ https://www.ncbi.nlm.nih.gov/pubmed/32319425 http://dx.doi.org/10.7554/eLife.54183 |
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