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Spatiotemporally precise optogenetic activation of sensory neurons in freely walking Drosophila

Previous work has characterized how walking Drosophila coordinate the movements of individual limbs (DeAngelis et al., 2019). To understand the circuit basis of this coordination, one must characterize how sensory feedback from each limb affects walking behavior. However, it has remained difficult t...

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Autores principales: DeAngelis, Brian D, Zavatone-Veth, Jacob A, Gonzalez-Suarez, Aneysis D, Clark, Damon A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7198233/
https://www.ncbi.nlm.nih.gov/pubmed/32319425
http://dx.doi.org/10.7554/eLife.54183
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author DeAngelis, Brian D
Zavatone-Veth, Jacob A
Gonzalez-Suarez, Aneysis D
Clark, Damon A
author_facet DeAngelis, Brian D
Zavatone-Veth, Jacob A
Gonzalez-Suarez, Aneysis D
Clark, Damon A
author_sort DeAngelis, Brian D
collection PubMed
description Previous work has characterized how walking Drosophila coordinate the movements of individual limbs (DeAngelis et al., 2019). To understand the circuit basis of this coordination, one must characterize how sensory feedback from each limb affects walking behavior. However, it has remained difficult to manipulate neural activity in individual limbs of freely moving animals. Here, we demonstrate a simple method for optogenetic stimulation with body side-, body segment-, and limb-specificity that does not require real-time tracking. Instead, we activate at random, precise locations in time and space and use post hoc analysis to determine behavioral responses to specific activations. Using this method, we have characterized limb coordination and walking behavior in response to transient activation of mechanosensitive bristle neurons and sweet-sensing chemoreceptor neurons. Our findings reveal that activating these neurons has opposite effects on turning, and that activations in different limbs and body regions produce distinct behaviors.
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spelling pubmed-71982332020-05-06 Spatiotemporally precise optogenetic activation of sensory neurons in freely walking Drosophila DeAngelis, Brian D Zavatone-Veth, Jacob A Gonzalez-Suarez, Aneysis D Clark, Damon A eLife Neuroscience Previous work has characterized how walking Drosophila coordinate the movements of individual limbs (DeAngelis et al., 2019). To understand the circuit basis of this coordination, one must characterize how sensory feedback from each limb affects walking behavior. However, it has remained difficult to manipulate neural activity in individual limbs of freely moving animals. Here, we demonstrate a simple method for optogenetic stimulation with body side-, body segment-, and limb-specificity that does not require real-time tracking. Instead, we activate at random, precise locations in time and space and use post hoc analysis to determine behavioral responses to specific activations. Using this method, we have characterized limb coordination and walking behavior in response to transient activation of mechanosensitive bristle neurons and sweet-sensing chemoreceptor neurons. Our findings reveal that activating these neurons has opposite effects on turning, and that activations in different limbs and body regions produce distinct behaviors. eLife Sciences Publications, Ltd 2020-04-22 /pmc/articles/PMC7198233/ /pubmed/32319425 http://dx.doi.org/10.7554/eLife.54183 Text en © 2020, DeAngelis et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
DeAngelis, Brian D
Zavatone-Veth, Jacob A
Gonzalez-Suarez, Aneysis D
Clark, Damon A
Spatiotemporally precise optogenetic activation of sensory neurons in freely walking Drosophila
title Spatiotemporally precise optogenetic activation of sensory neurons in freely walking Drosophila
title_full Spatiotemporally precise optogenetic activation of sensory neurons in freely walking Drosophila
title_fullStr Spatiotemporally precise optogenetic activation of sensory neurons in freely walking Drosophila
title_full_unstemmed Spatiotemporally precise optogenetic activation of sensory neurons in freely walking Drosophila
title_short Spatiotemporally precise optogenetic activation of sensory neurons in freely walking Drosophila
title_sort spatiotemporally precise optogenetic activation of sensory neurons in freely walking drosophila
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7198233/
https://www.ncbi.nlm.nih.gov/pubmed/32319425
http://dx.doi.org/10.7554/eLife.54183
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