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Alcohol Causes Lasting Differential Transcription in Drosophila Mushroom Body Neurons
Repeated alcohol experiences can produce long-lasting memories for sensory cues associated with intoxication. These memories can problematically trigger relapse in individuals recovering from alcohol use disorder (AUD). The molecular mechanisms by which ethanol changes memories to become long-lastin...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Genetics Society of America
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7198272/ https://www.ncbi.nlm.nih.gov/pubmed/32132098 http://dx.doi.org/10.1534/genetics.120.303101 |
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author | Petruccelli, Emily Brown, Tariq Waterman, Amanda Ledru, Nicolas Kaun, Karla R. |
author_facet | Petruccelli, Emily Brown, Tariq Waterman, Amanda Ledru, Nicolas Kaun, Karla R. |
author_sort | Petruccelli, Emily |
collection | PubMed |
description | Repeated alcohol experiences can produce long-lasting memories for sensory cues associated with intoxication. These memories can problematically trigger relapse in individuals recovering from alcohol use disorder (AUD). The molecular mechanisms by which ethanol changes memories to become long-lasting and inflexible remain unclear. New methods to analyze gene expression within precise neuronal cell types can provide further insight toward AUD prevention and treatment. Here, we used genetic tools in Drosophila melanogaster to investigate the lasting consequences of ethanol on transcription in memory-encoding neurons. Drosophila rely on mushroom body (MB) neurons to make associative memories, including memories of ethanol-associated sensory cues. Differential expression analyses revealed that distinct transcripts, but not genes, in the MB were associated with experiencing ethanol alone compared to forming a memory of an odor cue associated with ethanol. Adult MB-specific knockdown of spliceosome-associated proteins demonstrated the necessity of RNA-processing in ethanol memory formation. These findings highlight the dynamic, context-specific regulation of transcription in cue-encoding neurons, and the lasting effect of ethanol on transcript usage during memory formation. |
format | Online Article Text |
id | pubmed-7198272 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Genetics Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-71982722020-05-08 Alcohol Causes Lasting Differential Transcription in Drosophila Mushroom Body Neurons Petruccelli, Emily Brown, Tariq Waterman, Amanda Ledru, Nicolas Kaun, Karla R. Genetics Investigations Repeated alcohol experiences can produce long-lasting memories for sensory cues associated with intoxication. These memories can problematically trigger relapse in individuals recovering from alcohol use disorder (AUD). The molecular mechanisms by which ethanol changes memories to become long-lasting and inflexible remain unclear. New methods to analyze gene expression within precise neuronal cell types can provide further insight toward AUD prevention and treatment. Here, we used genetic tools in Drosophila melanogaster to investigate the lasting consequences of ethanol on transcription in memory-encoding neurons. Drosophila rely on mushroom body (MB) neurons to make associative memories, including memories of ethanol-associated sensory cues. Differential expression analyses revealed that distinct transcripts, but not genes, in the MB were associated with experiencing ethanol alone compared to forming a memory of an odor cue associated with ethanol. Adult MB-specific knockdown of spliceosome-associated proteins demonstrated the necessity of RNA-processing in ethanol memory formation. These findings highlight the dynamic, context-specific regulation of transcription in cue-encoding neurons, and the lasting effect of ethanol on transcript usage during memory formation. Genetics Society of America 2020-05 2020-03-04 /pmc/articles/PMC7198272/ /pubmed/32132098 http://dx.doi.org/10.1534/genetics.120.303101 Text en Copyright © 2020 by the Genetics Society of America Available freely online through the author-supported open access option. |
spellingShingle | Investigations Petruccelli, Emily Brown, Tariq Waterman, Amanda Ledru, Nicolas Kaun, Karla R. Alcohol Causes Lasting Differential Transcription in Drosophila Mushroom Body Neurons |
title | Alcohol Causes Lasting Differential Transcription in Drosophila Mushroom Body Neurons |
title_full | Alcohol Causes Lasting Differential Transcription in Drosophila Mushroom Body Neurons |
title_fullStr | Alcohol Causes Lasting Differential Transcription in Drosophila Mushroom Body Neurons |
title_full_unstemmed | Alcohol Causes Lasting Differential Transcription in Drosophila Mushroom Body Neurons |
title_short | Alcohol Causes Lasting Differential Transcription in Drosophila Mushroom Body Neurons |
title_sort | alcohol causes lasting differential transcription in drosophila mushroom body neurons |
topic | Investigations |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7198272/ https://www.ncbi.nlm.nih.gov/pubmed/32132098 http://dx.doi.org/10.1534/genetics.120.303101 |
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