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Feasibility and safety of focused ultrasound-enabled liquid biopsy in the brain of a porcine model
Although blood-based liquid biopsy is a promising noninvasive technique to acquire a comprehensive molecular tumor profile by detecting cancer-specific biomarkers (e.g. DNA, RNA, and proteins), there has been limited progress for brain tumor application partially because the low permeability of the...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7198482/ https://www.ncbi.nlm.nih.gov/pubmed/32366915 http://dx.doi.org/10.1038/s41598-020-64440-3 |
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author | Pacia, Christopher Pham Zhu, Lifei Yang, Yaoheng Yue, Yimei Nazeri, Arash Michael Gach, H. Talcott, Michael R. Leuthardt, Eric C. Chen, Hong |
author_facet | Pacia, Christopher Pham Zhu, Lifei Yang, Yaoheng Yue, Yimei Nazeri, Arash Michael Gach, H. Talcott, Michael R. Leuthardt, Eric C. Chen, Hong |
author_sort | Pacia, Christopher Pham |
collection | PubMed |
description | Although blood-based liquid biopsy is a promising noninvasive technique to acquire a comprehensive molecular tumor profile by detecting cancer-specific biomarkers (e.g. DNA, RNA, and proteins), there has been limited progress for brain tumor application partially because the low permeability of the blood-brain barrier (BBB) hinders the release of tumor biomarkers. We previously demonstrated focused ultrasound-enabled liquid biopsy (FUS-LBx) that uses FUS to increase BBB permeability in murine glioblastoma models and thus enhance the release of tumor-specific biomarkers into the bloodstream. The objective of this study was to evaluate the feasibility and safety of FUS-LBx in the normal brain tissue of a porcine model. Increased BBB permeability was confirmed by the significant increase (p = 0.0053) in K(trans) (the transfer coefficient from blood to brain extravascular extracellular space) when comparing the FUS-sonicated brain area with the contralateral non-sonicated area. Meanwhile, there was a significant increase in the blood concentrations of glial fibrillary acidic protein (GFAP, p = 0.0074) and myelin basic protein (MBP, p = 0.0039) after FUS sonication as compared with before FUS. There was no detectable tissue damage by T(2)(*)-weighted MRI and histological analysis. Findings from this study suggest that FUS-LBx is a promising technique for noninvasive and localized diagnosis of the molecular profiles of brain diseases with the potential to translate to the clinic. |
format | Online Article Text |
id | pubmed-7198482 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-71984822020-05-08 Feasibility and safety of focused ultrasound-enabled liquid biopsy in the brain of a porcine model Pacia, Christopher Pham Zhu, Lifei Yang, Yaoheng Yue, Yimei Nazeri, Arash Michael Gach, H. Talcott, Michael R. Leuthardt, Eric C. Chen, Hong Sci Rep Article Although blood-based liquid biopsy is a promising noninvasive technique to acquire a comprehensive molecular tumor profile by detecting cancer-specific biomarkers (e.g. DNA, RNA, and proteins), there has been limited progress for brain tumor application partially because the low permeability of the blood-brain barrier (BBB) hinders the release of tumor biomarkers. We previously demonstrated focused ultrasound-enabled liquid biopsy (FUS-LBx) that uses FUS to increase BBB permeability in murine glioblastoma models and thus enhance the release of tumor-specific biomarkers into the bloodstream. The objective of this study was to evaluate the feasibility and safety of FUS-LBx in the normal brain tissue of a porcine model. Increased BBB permeability was confirmed by the significant increase (p = 0.0053) in K(trans) (the transfer coefficient from blood to brain extravascular extracellular space) when comparing the FUS-sonicated brain area with the contralateral non-sonicated area. Meanwhile, there was a significant increase in the blood concentrations of glial fibrillary acidic protein (GFAP, p = 0.0074) and myelin basic protein (MBP, p = 0.0039) after FUS sonication as compared with before FUS. There was no detectable tissue damage by T(2)(*)-weighted MRI and histological analysis. Findings from this study suggest that FUS-LBx is a promising technique for noninvasive and localized diagnosis of the molecular profiles of brain diseases with the potential to translate to the clinic. Nature Publishing Group UK 2020-05-04 /pmc/articles/PMC7198482/ /pubmed/32366915 http://dx.doi.org/10.1038/s41598-020-64440-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Pacia, Christopher Pham Zhu, Lifei Yang, Yaoheng Yue, Yimei Nazeri, Arash Michael Gach, H. Talcott, Michael R. Leuthardt, Eric C. Chen, Hong Feasibility and safety of focused ultrasound-enabled liquid biopsy in the brain of a porcine model |
title | Feasibility and safety of focused ultrasound-enabled liquid biopsy in the brain of a porcine model |
title_full | Feasibility and safety of focused ultrasound-enabled liquid biopsy in the brain of a porcine model |
title_fullStr | Feasibility and safety of focused ultrasound-enabled liquid biopsy in the brain of a porcine model |
title_full_unstemmed | Feasibility and safety of focused ultrasound-enabled liquid biopsy in the brain of a porcine model |
title_short | Feasibility and safety of focused ultrasound-enabled liquid biopsy in the brain of a porcine model |
title_sort | feasibility and safety of focused ultrasound-enabled liquid biopsy in the brain of a porcine model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7198482/ https://www.ncbi.nlm.nih.gov/pubmed/32366915 http://dx.doi.org/10.1038/s41598-020-64440-3 |
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