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Identification and characterisation of enteroaggregative Escherichia coli subtypes associated with human disease

Enteroaggregative E. coli (EAEC) are a major cause of diarrhoea worldwide. Due to their heterogeneity and carriage in healthy individuals, identification of diagnostic virulence markers for pathogenic strains has been difficult. In this study, we have determined phenotypic and genotypic differences...

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Autores principales: Ellis, Samuel J., Crossman, Lisa C., McGrath, Conor J., Chattaway, Marie A., Hölken, Johanna M., Brett, Bernard, Bundy, Leah, Kay, Gemma L., Wain, John, Schüller, Stephanie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7198487/
https://www.ncbi.nlm.nih.gov/pubmed/32366874
http://dx.doi.org/10.1038/s41598-020-64424-3
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author Ellis, Samuel J.
Crossman, Lisa C.
McGrath, Conor J.
Chattaway, Marie A.
Hölken, Johanna M.
Brett, Bernard
Bundy, Leah
Kay, Gemma L.
Wain, John
Schüller, Stephanie
author_facet Ellis, Samuel J.
Crossman, Lisa C.
McGrath, Conor J.
Chattaway, Marie A.
Hölken, Johanna M.
Brett, Bernard
Bundy, Leah
Kay, Gemma L.
Wain, John
Schüller, Stephanie
author_sort Ellis, Samuel J.
collection PubMed
description Enteroaggregative E. coli (EAEC) are a major cause of diarrhoea worldwide. Due to their heterogeneity and carriage in healthy individuals, identification of diagnostic virulence markers for pathogenic strains has been difficult. In this study, we have determined phenotypic and genotypic differences between EAEC strains of sequence types (STs) epidemiologically associated with asymptomatic carriage (ST31) and diarrhoeal disease (ST40). ST40 strains demonstrated significantly enhanced intestinal adherence, biofilm formation, and pro-inflammatory interleukin-8 secretion compared with ST31 isolates. This was independent of whether strains were derived from diarrhoea patients or healthy controls. Whole genome sequencing revealed differences in putative virulence genes encoding aggregative adherence fimbriae, E. coli common pilus, flagellin and EAEC heat-stable enterotoxin 1. Our results indicate that ST40 strains have a higher intrinsic potential of human pathogenesis due to a specific combination of virulence-related factors which promote host cell colonization and inflammation. These findings may contribute to the development of genotypic and/or phenotypic markers for EAEC strains of high virulence.
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spelling pubmed-71984872020-05-08 Identification and characterisation of enteroaggregative Escherichia coli subtypes associated with human disease Ellis, Samuel J. Crossman, Lisa C. McGrath, Conor J. Chattaway, Marie A. Hölken, Johanna M. Brett, Bernard Bundy, Leah Kay, Gemma L. Wain, John Schüller, Stephanie Sci Rep Article Enteroaggregative E. coli (EAEC) are a major cause of diarrhoea worldwide. Due to their heterogeneity and carriage in healthy individuals, identification of diagnostic virulence markers for pathogenic strains has been difficult. In this study, we have determined phenotypic and genotypic differences between EAEC strains of sequence types (STs) epidemiologically associated with asymptomatic carriage (ST31) and diarrhoeal disease (ST40). ST40 strains demonstrated significantly enhanced intestinal adherence, biofilm formation, and pro-inflammatory interleukin-8 secretion compared with ST31 isolates. This was independent of whether strains were derived from diarrhoea patients or healthy controls. Whole genome sequencing revealed differences in putative virulence genes encoding aggregative adherence fimbriae, E. coli common pilus, flagellin and EAEC heat-stable enterotoxin 1. Our results indicate that ST40 strains have a higher intrinsic potential of human pathogenesis due to a specific combination of virulence-related factors which promote host cell colonization and inflammation. These findings may contribute to the development of genotypic and/or phenotypic markers for EAEC strains of high virulence. Nature Publishing Group UK 2020-05-04 /pmc/articles/PMC7198487/ /pubmed/32366874 http://dx.doi.org/10.1038/s41598-020-64424-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ellis, Samuel J.
Crossman, Lisa C.
McGrath, Conor J.
Chattaway, Marie A.
Hölken, Johanna M.
Brett, Bernard
Bundy, Leah
Kay, Gemma L.
Wain, John
Schüller, Stephanie
Identification and characterisation of enteroaggregative Escherichia coli subtypes associated with human disease
title Identification and characterisation of enteroaggregative Escherichia coli subtypes associated with human disease
title_full Identification and characterisation of enteroaggregative Escherichia coli subtypes associated with human disease
title_fullStr Identification and characterisation of enteroaggregative Escherichia coli subtypes associated with human disease
title_full_unstemmed Identification and characterisation of enteroaggregative Escherichia coli subtypes associated with human disease
title_short Identification and characterisation of enteroaggregative Escherichia coli subtypes associated with human disease
title_sort identification and characterisation of enteroaggregative escherichia coli subtypes associated with human disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7198487/
https://www.ncbi.nlm.nih.gov/pubmed/32366874
http://dx.doi.org/10.1038/s41598-020-64424-3
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