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microRNA Expression in Women With and Without Polycystic Ovarian Syndrome Matched for Body Mass Index
Background: Despite several authors who have hypothesized that alterations of small noncoding RNAs (miR) are implicated in the etiopathogenesis of polycystic ovarian syndrome (PCOS), contrasting findings have been reported so far. Discrepancies in body mass index (BMI) levels may account for these d...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7199502/ https://www.ncbi.nlm.nih.gov/pubmed/32411089 http://dx.doi.org/10.3389/fendo.2020.00206 |
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author | Butler, Alexandra E. Ramachandran, Vimal Sathyapalan, Thozhukat David, Rhiannon Gooderham, Nigel J. Benurwar, Manasi Dargham, Soha R. Hayat, Shahina Hani Najafi-Shoushtari, S. Atkin, Stephen L. |
author_facet | Butler, Alexandra E. Ramachandran, Vimal Sathyapalan, Thozhukat David, Rhiannon Gooderham, Nigel J. Benurwar, Manasi Dargham, Soha R. Hayat, Shahina Hani Najafi-Shoushtari, S. Atkin, Stephen L. |
author_sort | Butler, Alexandra E. |
collection | PubMed |
description | Background: Despite several authors who have hypothesized that alterations of small noncoding RNAs (miR) are implicated in the etiopathogenesis of polycystic ovarian syndrome (PCOS), contrasting findings have been reported so far. Discrepancies in body mass index (BMI) levels may account for these differences; therefore, the aim of the present study was to determine whether miR differed in serum samples collected from age- and BMI-matched control and PCOS women. Methods: In a cross-sectional study, miR were measured using quantitative polymerase chain reaction in 29 women with anovulatory PCOS women and 29 control women who were in the follicular phase of their menstrual cycle, from the local biobank. Results: One hundred seventy-six miR were detected, of which 15 miR passed the false discovery rate (FDR; p < 0.05) that differed between PCOS and control women. There was no association of the top 9 miR (p < 0.02) (miR-486-5p, miR-24-3p, miR-19b-3p, miR-22-3p, miR-19a-3p, miR-339-5p, miR-185-5p, miR-101-3p, miR-let-7i-5p) with BMI, androgen levels, insulin resistance, or antimullerian hormone (AMH) in either PCOS or normal women. Ingenuity pathway assessment showed the pathways were interrelated for abnormalities of the reproductive system. Conclusion: When the confounding influence of weight was accounted for, miR levels differed between anovulatory PCOS women and control women in the follicular phase of the menstrual cycle. Interestingly, the differing miR were associated with the pathways of reproductive abnormalities but did not associate with AMH or metabolic parameters. |
format | Online Article Text |
id | pubmed-7199502 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71995022020-05-14 microRNA Expression in Women With and Without Polycystic Ovarian Syndrome Matched for Body Mass Index Butler, Alexandra E. Ramachandran, Vimal Sathyapalan, Thozhukat David, Rhiannon Gooderham, Nigel J. Benurwar, Manasi Dargham, Soha R. Hayat, Shahina Hani Najafi-Shoushtari, S. Atkin, Stephen L. Front Endocrinol (Lausanne) Endocrinology Background: Despite several authors who have hypothesized that alterations of small noncoding RNAs (miR) are implicated in the etiopathogenesis of polycystic ovarian syndrome (PCOS), contrasting findings have been reported so far. Discrepancies in body mass index (BMI) levels may account for these differences; therefore, the aim of the present study was to determine whether miR differed in serum samples collected from age- and BMI-matched control and PCOS women. Methods: In a cross-sectional study, miR were measured using quantitative polymerase chain reaction in 29 women with anovulatory PCOS women and 29 control women who were in the follicular phase of their menstrual cycle, from the local biobank. Results: One hundred seventy-six miR were detected, of which 15 miR passed the false discovery rate (FDR; p < 0.05) that differed between PCOS and control women. There was no association of the top 9 miR (p < 0.02) (miR-486-5p, miR-24-3p, miR-19b-3p, miR-22-3p, miR-19a-3p, miR-339-5p, miR-185-5p, miR-101-3p, miR-let-7i-5p) with BMI, androgen levels, insulin resistance, or antimullerian hormone (AMH) in either PCOS or normal women. Ingenuity pathway assessment showed the pathways were interrelated for abnormalities of the reproductive system. Conclusion: When the confounding influence of weight was accounted for, miR levels differed between anovulatory PCOS women and control women in the follicular phase of the menstrual cycle. Interestingly, the differing miR were associated with the pathways of reproductive abnormalities but did not associate with AMH or metabolic parameters. Frontiers Media S.A. 2020-04-28 /pmc/articles/PMC7199502/ /pubmed/32411089 http://dx.doi.org/10.3389/fendo.2020.00206 Text en Copyright © 2020 Butler, Ramachandran, Sathyapalan, David, Gooderham, Benurwar, Dargham, Hayat, Hani Najafi-Shoushtari and Atkin. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Endocrinology Butler, Alexandra E. Ramachandran, Vimal Sathyapalan, Thozhukat David, Rhiannon Gooderham, Nigel J. Benurwar, Manasi Dargham, Soha R. Hayat, Shahina Hani Najafi-Shoushtari, S. Atkin, Stephen L. microRNA Expression in Women With and Without Polycystic Ovarian Syndrome Matched for Body Mass Index |
title | microRNA Expression in Women With and Without Polycystic Ovarian Syndrome Matched for Body Mass Index |
title_full | microRNA Expression in Women With and Without Polycystic Ovarian Syndrome Matched for Body Mass Index |
title_fullStr | microRNA Expression in Women With and Without Polycystic Ovarian Syndrome Matched for Body Mass Index |
title_full_unstemmed | microRNA Expression in Women With and Without Polycystic Ovarian Syndrome Matched for Body Mass Index |
title_short | microRNA Expression in Women With and Without Polycystic Ovarian Syndrome Matched for Body Mass Index |
title_sort | microrna expression in women with and without polycystic ovarian syndrome matched for body mass index |
topic | Endocrinology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7199502/ https://www.ncbi.nlm.nih.gov/pubmed/32411089 http://dx.doi.org/10.3389/fendo.2020.00206 |
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