Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo

Understanding information processing in the brain requires us to monitor neural activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope (2PFM) empowered by all-optical laser scanning, we imaged neural activity in vivo at up to 3,000 frames per second and sub...

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Detalles Bibliográficos
Autores principales: Wu, Jianglai, Liang, Yajie, Chen, Shuo, Hsu, Ching-Lung, Chavarha, Mariya, Evans, Stephen W, Shi, Dongqing, Lin, Michael Z, Tsia, Kevin K, Ji, Na
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7199528/
https://www.ncbi.nlm.nih.gov/pubmed/32123392
http://dx.doi.org/10.1038/s41592-020-0762-7
Descripción
Sumario:Understanding information processing in the brain requires us to monitor neural activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope (2PFM) empowered by all-optical laser scanning, we imaged neural activity in vivo at up to 3,000 frames per second and submicron spatial resolution. This ultrafast imaging method enabled monitoring of both supra- and sub-threshold electrical activity down to 345 μm below the brain surface in head-fixed awake mice.

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