Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo

Understanding information processing in the brain requires us to monitor neural activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope (2PFM) empowered by all-optical laser scanning, we imaged neural activity in vivo at up to 3,000 frames per second and sub...

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Detalles Bibliográficos
Autores principales: Wu, Jianglai, Liang, Yajie, Chen, Shuo, Hsu, Ching-Lung, Chavarha, Mariya, Evans, Stephen W, Shi, Dongqing, Lin, Michael Z, Tsia, Kevin K, Ji, Na
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7199528/
https://www.ncbi.nlm.nih.gov/pubmed/32123392
http://dx.doi.org/10.1038/s41592-020-0762-7
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author Wu, Jianglai
Liang, Yajie
Chen, Shuo
Hsu, Ching-Lung
Chavarha, Mariya
Evans, Stephen W
Shi, Dongqing
Lin, Michael Z
Tsia, Kevin K
Ji, Na
author_facet Wu, Jianglai
Liang, Yajie
Chen, Shuo
Hsu, Ching-Lung
Chavarha, Mariya
Evans, Stephen W
Shi, Dongqing
Lin, Michael Z
Tsia, Kevin K
Ji, Na
author_sort Wu, Jianglai
collection PubMed
description Understanding information processing in the brain requires us to monitor neural activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope (2PFM) empowered by all-optical laser scanning, we imaged neural activity in vivo at up to 3,000 frames per second and submicron spatial resolution. This ultrafast imaging method enabled monitoring of both supra- and sub-threshold electrical activity down to 345 μm below the brain surface in head-fixed awake mice.
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spelling pubmed-71995282020-09-02 Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo Wu, Jianglai Liang, Yajie Chen, Shuo Hsu, Ching-Lung Chavarha, Mariya Evans, Stephen W Shi, Dongqing Lin, Michael Z Tsia, Kevin K Ji, Na Nat Methods Article Understanding information processing in the brain requires us to monitor neural activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope (2PFM) empowered by all-optical laser scanning, we imaged neural activity in vivo at up to 3,000 frames per second and submicron spatial resolution. This ultrafast imaging method enabled monitoring of both supra- and sub-threshold electrical activity down to 345 μm below the brain surface in head-fixed awake mice. 2020-03-02 2020-03 /pmc/articles/PMC7199528/ /pubmed/32123392 http://dx.doi.org/10.1038/s41592-020-0762-7 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Wu, Jianglai
Liang, Yajie
Chen, Shuo
Hsu, Ching-Lung
Chavarha, Mariya
Evans, Stephen W
Shi, Dongqing
Lin, Michael Z
Tsia, Kevin K
Ji, Na
Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo
title Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo
title_full Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo
title_fullStr Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo
title_full_unstemmed Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo
title_short Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo
title_sort kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7199528/
https://www.ncbi.nlm.nih.gov/pubmed/32123392
http://dx.doi.org/10.1038/s41592-020-0762-7
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