nanoMLST: accurate multilocus sequence typing using Oxford Nanopore Technologies MinION with a dual-barcode approach to multiplex large numbers of samples

Multilocus sequence typing (MLST) is one of the most commonly used methods for studying microbial lineage worldwide. However, the traditional MLST process using Sanger sequencing is time-consuming and expensive. We have designed a workflow that simultaneously sequenced seven full-length housekeeping...

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Detalles Bibliográficos
Autores principales: Liou, Ci-Hong, Wu, Han-Chieh, Liao, Yu-Chieh, Yang Lauderdale, Tsai-Ling, Huang, I-Wen, Chen, Feng-Jui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2020
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200061/
https://www.ncbi.nlm.nih.gov/pubmed/32065578
http://dx.doi.org/10.1099/mgen.0.000336

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