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DNA-PAINT super-resolution imaging data of surface exposed active sites on particles

Surface functionalization with targeting ligands confers to nanomaterials the ability of selectively recognize a biological target. Therefore, a quantitative characterization of surface functional molecules is critical for the rational development of nanomaterials-based applications, especially in n...

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Detalles Bibliográficos
Autores principales: Delcanale, Pietro, Albertazzi, Lorenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200214/
https://www.ncbi.nlm.nih.gov/pubmed/32382590
http://dx.doi.org/10.1016/j.dib.2020.105468
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author Delcanale, Pietro
Albertazzi, Lorenzo
author_facet Delcanale, Pietro
Albertazzi, Lorenzo
author_sort Delcanale, Pietro
collection PubMed
description Surface functionalization with targeting ligands confers to nanomaterials the ability of selectively recognize a biological target. Therefore, a quantitative characterization of surface functional molecules is critical for the rational development of nanomaterials-based applications, especially in nanomedicine research. Single-molecule localization microscopy can provide visualization of surface molecules at the level of individual particles, preserving the integrity of the material and overcoming the limitations of analytical methods based on ensemble averaging. Here we provide single-molecule localization data obtained on streptavidin-coated polystyrene particles, which can be exploited as a model system for surface-functionalized materials. After loading of the active sites of streptavidin molecules with a biotin-conjugated probe, they were imaged with a DNA-PAINT imaging approach, which can provide single-molecule imaging at subdiffraction resolution and molecule counting. Both raw records and analysed data, consisting in a list of space-time single-molecule coordinates, are shared. Additionally, Matlab functions are provided that analyse the single-molecule coordinates in order to quantify features of individual particles. These data might constitute a valuable reference for applications of similar quantitative imaging methodologies to other types of functionalized nanomaterials.
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spelling pubmed-72002142020-05-07 DNA-PAINT super-resolution imaging data of surface exposed active sites on particles Delcanale, Pietro Albertazzi, Lorenzo Data Brief Biochemistry, Genetics and Molecular Biology Surface functionalization with targeting ligands confers to nanomaterials the ability of selectively recognize a biological target. Therefore, a quantitative characterization of surface functional molecules is critical for the rational development of nanomaterials-based applications, especially in nanomedicine research. Single-molecule localization microscopy can provide visualization of surface molecules at the level of individual particles, preserving the integrity of the material and overcoming the limitations of analytical methods based on ensemble averaging. Here we provide single-molecule localization data obtained on streptavidin-coated polystyrene particles, which can be exploited as a model system for surface-functionalized materials. After loading of the active sites of streptavidin molecules with a biotin-conjugated probe, they were imaged with a DNA-PAINT imaging approach, which can provide single-molecule imaging at subdiffraction resolution and molecule counting. Both raw records and analysed data, consisting in a list of space-time single-molecule coordinates, are shared. Additionally, Matlab functions are provided that analyse the single-molecule coordinates in order to quantify features of individual particles. These data might constitute a valuable reference for applications of similar quantitative imaging methodologies to other types of functionalized nanomaterials. Elsevier 2020-04-08 /pmc/articles/PMC7200214/ /pubmed/32382590 http://dx.doi.org/10.1016/j.dib.2020.105468 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Biochemistry, Genetics and Molecular Biology
Delcanale, Pietro
Albertazzi, Lorenzo
DNA-PAINT super-resolution imaging data of surface exposed active sites on particles
title DNA-PAINT super-resolution imaging data of surface exposed active sites on particles
title_full DNA-PAINT super-resolution imaging data of surface exposed active sites on particles
title_fullStr DNA-PAINT super-resolution imaging data of surface exposed active sites on particles
title_full_unstemmed DNA-PAINT super-resolution imaging data of surface exposed active sites on particles
title_short DNA-PAINT super-resolution imaging data of surface exposed active sites on particles
title_sort dna-paint super-resolution imaging data of surface exposed active sites on particles
topic Biochemistry, Genetics and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200214/
https://www.ncbi.nlm.nih.gov/pubmed/32382590
http://dx.doi.org/10.1016/j.dib.2020.105468
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