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DNA-PAINT super-resolution imaging data of surface exposed active sites on particles
Surface functionalization with targeting ligands confers to nanomaterials the ability of selectively recognize a biological target. Therefore, a quantitative characterization of surface functional molecules is critical for the rational development of nanomaterials-based applications, especially in n...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200214/ https://www.ncbi.nlm.nih.gov/pubmed/32382590 http://dx.doi.org/10.1016/j.dib.2020.105468 |
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author | Delcanale, Pietro Albertazzi, Lorenzo |
author_facet | Delcanale, Pietro Albertazzi, Lorenzo |
author_sort | Delcanale, Pietro |
collection | PubMed |
description | Surface functionalization with targeting ligands confers to nanomaterials the ability of selectively recognize a biological target. Therefore, a quantitative characterization of surface functional molecules is critical for the rational development of nanomaterials-based applications, especially in nanomedicine research. Single-molecule localization microscopy can provide visualization of surface molecules at the level of individual particles, preserving the integrity of the material and overcoming the limitations of analytical methods based on ensemble averaging. Here we provide single-molecule localization data obtained on streptavidin-coated polystyrene particles, which can be exploited as a model system for surface-functionalized materials. After loading of the active sites of streptavidin molecules with a biotin-conjugated probe, they were imaged with a DNA-PAINT imaging approach, which can provide single-molecule imaging at subdiffraction resolution and molecule counting. Both raw records and analysed data, consisting in a list of space-time single-molecule coordinates, are shared. Additionally, Matlab functions are provided that analyse the single-molecule coordinates in order to quantify features of individual particles. These data might constitute a valuable reference for applications of similar quantitative imaging methodologies to other types of functionalized nanomaterials. |
format | Online Article Text |
id | pubmed-7200214 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-72002142020-05-07 DNA-PAINT super-resolution imaging data of surface exposed active sites on particles Delcanale, Pietro Albertazzi, Lorenzo Data Brief Biochemistry, Genetics and Molecular Biology Surface functionalization with targeting ligands confers to nanomaterials the ability of selectively recognize a biological target. Therefore, a quantitative characterization of surface functional molecules is critical for the rational development of nanomaterials-based applications, especially in nanomedicine research. Single-molecule localization microscopy can provide visualization of surface molecules at the level of individual particles, preserving the integrity of the material and overcoming the limitations of analytical methods based on ensemble averaging. Here we provide single-molecule localization data obtained on streptavidin-coated polystyrene particles, which can be exploited as a model system for surface-functionalized materials. After loading of the active sites of streptavidin molecules with a biotin-conjugated probe, they were imaged with a DNA-PAINT imaging approach, which can provide single-molecule imaging at subdiffraction resolution and molecule counting. Both raw records and analysed data, consisting in a list of space-time single-molecule coordinates, are shared. Additionally, Matlab functions are provided that analyse the single-molecule coordinates in order to quantify features of individual particles. These data might constitute a valuable reference for applications of similar quantitative imaging methodologies to other types of functionalized nanomaterials. Elsevier 2020-04-08 /pmc/articles/PMC7200214/ /pubmed/32382590 http://dx.doi.org/10.1016/j.dib.2020.105468 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Biochemistry, Genetics and Molecular Biology Delcanale, Pietro Albertazzi, Lorenzo DNA-PAINT super-resolution imaging data of surface exposed active sites on particles |
title | DNA-PAINT super-resolution imaging data of surface exposed active sites on particles |
title_full | DNA-PAINT super-resolution imaging data of surface exposed active sites on particles |
title_fullStr | DNA-PAINT super-resolution imaging data of surface exposed active sites on particles |
title_full_unstemmed | DNA-PAINT super-resolution imaging data of surface exposed active sites on particles |
title_short | DNA-PAINT super-resolution imaging data of surface exposed active sites on particles |
title_sort | dna-paint super-resolution imaging data of surface exposed active sites on particles |
topic | Biochemistry, Genetics and Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200214/ https://www.ncbi.nlm.nih.gov/pubmed/32382590 http://dx.doi.org/10.1016/j.dib.2020.105468 |
work_keys_str_mv | AT delcanalepietro dnapaintsuperresolutionimagingdataofsurfaceexposedactivesitesonparticles AT albertazzilorenzo dnapaintsuperresolutionimagingdataofsurfaceexposedactivesitesonparticles |