Effect of increased inclusion of dried distillers grain supplement on adaptation, intake, digestibility, and rumen parameters in steers consuming bermudagrass round bale silage

Eight ruminally cannulated beef steers were used to evaluate forage intake and physiological response during adaptation to a diet consisting of bermudagrass round bale silage (RBS) and dried distillers grains (DDG) and in a second experiment the effect of level of DDG supplementation on RBS forage intake, digestibility, and rumen parameters. The adaptation experiment was designed to simulate a 14-d step-up process. Initially steers received ad libitum RBS, on d 4 steers were offered 1.13 kg of DDG daily and every 4 d daily DDG offered was increased by 1.13 kg. Blood and rumen fluid samples were collected before feeding (0 h) and 6, 12, 24 (before feeding), and 36 h after every diet change. The digestibility experiment was conducted as an 8 × 4 Latin square with four periods. Treatments included RBS only and DDG supplement levels of 0.33%, 0.66%, and 1.0% of body weight (RBS + 0.33, +0.66, and 1.0, respectively). Collection periods consisted of 21 d to determine dry matter intake [DMI], digestibility, and collect rumen fluid samples. During adaptation, level of DDG did not (P = 0.42) affect RBS DMI. Mean ruminal pH was greater (P < 0.05) for RBS only compared with diets with DDG. When steers were offered 3.39 or 4.52 kg/d of DDG rumen NH(3)-N concentration was greater (P ≤ 0.05) compared with other levels of DDG or RBS alone. Mean nonesterified fatty acid and plasma urea N concentrations varied among levels of DDG offered. In the digestibility experiment forage DMI, total DMI, and total tract apparent digestibility exhibited a quadratic (P ≤ 0.05) response to level of DDG offered. Mean ruminal pH responded in a quadratic manner (P = 0.03) and was greater for RBS only compared with RBS plus DDG at any level. Mean ruminal NH(3)-N concentration tended (P = 0.07) to respond in a cubic manner as DDG amount offered increased. Physiological responses during adaptation to DDG over 14 d resembled responses previously observed after long-term DDG supplementation. Offering DDG up to 1% of body weight did depress forage DMI and ruminal pH but increased total DMI, diet digestibility, and ruminal NH(3)-N concentration.